Systematic analysis of tumour cell-extracellular matrix adhesion identifies independent prognostic factors in breast cancer

Jason R. Todd, Karen A. Ryall, Simon Vyse, Jocelyn P. Wong, Rachael C. Natrajan, Yinyin Yuan, Aik-Choon Tan, Paul H. Huang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tumour cell-extracellular matrix (ECM) interactions are fundamental for discrete steps in breast cancer progression. In particular, cancer cell adhesion to ECM proteins present in the microenvironment is critical for accelerating tumour growth and facilitating metastatic spread. To assess the utility of tumour cell-ECM adhesion as a means for discovering prognostic factors in breast cancer survival, here we perform a systematic phenotypic screen and characterise the adhesion properties of a panel of human HER2 amplified breast cancer cell lines across six ECM proteins commonly deregulated in breast cancer. We determine a gene expression signature that defines a subset of cell lines displaying impaired adhesion to laminin. Cells with impaired laminin adhesion showed an enrichment in genes associated with cell motility and molecular pathways linked to cytokine signalling and inflammation. Evaluation of this gene set in the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) cohort of 1,964 patients identifies the F12 and STC2 genes as independent prognostic factors for overall survival in breast cancer. Our study demonstrates the potential of in vitro cell adhesion screens as a novel approach for identifying prognostic factors for disease outcome.

Original languageEnglish
Pages (from-to)62939-62953
Number of pages15
JournalOncotarget
Volume7
Issue number39
DOIs
Publication statusPublished - 2016
Externally publishedYes

Fingerprint

Cell-Matrix Junctions
Extracellular Matrix
Breast Neoplasms
Neoplasms
Extracellular Matrix Proteins
Laminin
Cell Adhesion
Genes
Cell Line
Survival
Transcriptome
Cell Movement
Cytokines
Inflammation
Growth

Keywords

  • Breast cancer
  • Cell adhesion
  • Extracellular matrix
  • HER2
  • Laminin

ASJC Scopus subject areas

  • Oncology

Cite this

Todd, J. R., Ryall, K. A., Vyse, S., Wong, J. P., Natrajan, R. C., Yuan, Y., ... Huang, P. H. (2016). Systematic analysis of tumour cell-extracellular matrix adhesion identifies independent prognostic factors in breast cancer. Oncotarget, 7(39), 62939-62953. https://doi.org/10.18632/oncotarget.11307

Systematic analysis of tumour cell-extracellular matrix adhesion identifies independent prognostic factors in breast cancer. / Todd, Jason R.; Ryall, Karen A.; Vyse, Simon; Wong, Jocelyn P.; Natrajan, Rachael C.; Yuan, Yinyin; Tan, Aik-Choon; Huang, Paul H.

In: Oncotarget, Vol. 7, No. 39, 2016, p. 62939-62953.

Research output: Contribution to journalArticle

Todd, JR, Ryall, KA, Vyse, S, Wong, JP, Natrajan, RC, Yuan, Y, Tan, A-C & Huang, PH 2016, 'Systematic analysis of tumour cell-extracellular matrix adhesion identifies independent prognostic factors in breast cancer', Oncotarget, vol. 7, no. 39, pp. 62939-62953. https://doi.org/10.18632/oncotarget.11307
Todd, Jason R. ; Ryall, Karen A. ; Vyse, Simon ; Wong, Jocelyn P. ; Natrajan, Rachael C. ; Yuan, Yinyin ; Tan, Aik-Choon ; Huang, Paul H. / Systematic analysis of tumour cell-extracellular matrix adhesion identifies independent prognostic factors in breast cancer. In: Oncotarget. 2016 ; Vol. 7, No. 39. pp. 62939-62953.
@article{dff205572b3b48ceaa467b51d7cac20c,
title = "Systematic analysis of tumour cell-extracellular matrix adhesion identifies independent prognostic factors in breast cancer",
abstract = "Tumour cell-extracellular matrix (ECM) interactions are fundamental for discrete steps in breast cancer progression. In particular, cancer cell adhesion to ECM proteins present in the microenvironment is critical for accelerating tumour growth and facilitating metastatic spread. To assess the utility of tumour cell-ECM adhesion as a means for discovering prognostic factors in breast cancer survival, here we perform a systematic phenotypic screen and characterise the adhesion properties of a panel of human HER2 amplified breast cancer cell lines across six ECM proteins commonly deregulated in breast cancer. We determine a gene expression signature that defines a subset of cell lines displaying impaired adhesion to laminin. Cells with impaired laminin adhesion showed an enrichment in genes associated with cell motility and molecular pathways linked to cytokine signalling and inflammation. Evaluation of this gene set in the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) cohort of 1,964 patients identifies the F12 and STC2 genes as independent prognostic factors for overall survival in breast cancer. Our study demonstrates the potential of in vitro cell adhesion screens as a novel approach for identifying prognostic factors for disease outcome.",
keywords = "Breast cancer, Cell adhesion, Extracellular matrix, HER2, Laminin",
author = "Todd, {Jason R.} and Ryall, {Karen A.} and Simon Vyse and Wong, {Jocelyn P.} and Natrajan, {Rachael C.} and Yinyin Yuan and Aik-Choon Tan and Huang, {Paul H.}",
year = "2016",
doi = "10.18632/oncotarget.11307",
language = "English",
volume = "7",
pages = "62939--62953",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals",
number = "39",

}

TY - JOUR

T1 - Systematic analysis of tumour cell-extracellular matrix adhesion identifies independent prognostic factors in breast cancer

AU - Todd, Jason R.

AU - Ryall, Karen A.

AU - Vyse, Simon

AU - Wong, Jocelyn P.

AU - Natrajan, Rachael C.

AU - Yuan, Yinyin

AU - Tan, Aik-Choon

AU - Huang, Paul H.

PY - 2016

Y1 - 2016

N2 - Tumour cell-extracellular matrix (ECM) interactions are fundamental for discrete steps in breast cancer progression. In particular, cancer cell adhesion to ECM proteins present in the microenvironment is critical for accelerating tumour growth and facilitating metastatic spread. To assess the utility of tumour cell-ECM adhesion as a means for discovering prognostic factors in breast cancer survival, here we perform a systematic phenotypic screen and characterise the adhesion properties of a panel of human HER2 amplified breast cancer cell lines across six ECM proteins commonly deregulated in breast cancer. We determine a gene expression signature that defines a subset of cell lines displaying impaired adhesion to laminin. Cells with impaired laminin adhesion showed an enrichment in genes associated with cell motility and molecular pathways linked to cytokine signalling and inflammation. Evaluation of this gene set in the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) cohort of 1,964 patients identifies the F12 and STC2 genes as independent prognostic factors for overall survival in breast cancer. Our study demonstrates the potential of in vitro cell adhesion screens as a novel approach for identifying prognostic factors for disease outcome.

AB - Tumour cell-extracellular matrix (ECM) interactions are fundamental for discrete steps in breast cancer progression. In particular, cancer cell adhesion to ECM proteins present in the microenvironment is critical for accelerating tumour growth and facilitating metastatic spread. To assess the utility of tumour cell-ECM adhesion as a means for discovering prognostic factors in breast cancer survival, here we perform a systematic phenotypic screen and characterise the adhesion properties of a panel of human HER2 amplified breast cancer cell lines across six ECM proteins commonly deregulated in breast cancer. We determine a gene expression signature that defines a subset of cell lines displaying impaired adhesion to laminin. Cells with impaired laminin adhesion showed an enrichment in genes associated with cell motility and molecular pathways linked to cytokine signalling and inflammation. Evaluation of this gene set in the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) cohort of 1,964 patients identifies the F12 and STC2 genes as independent prognostic factors for overall survival in breast cancer. Our study demonstrates the potential of in vitro cell adhesion screens as a novel approach for identifying prognostic factors for disease outcome.

KW - Breast cancer

KW - Cell adhesion

KW - Extracellular matrix

KW - HER2

KW - Laminin

UR - http://www.scopus.com/inward/record.url?scp=84993990837&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84993990837&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.11307

DO - 10.18632/oncotarget.11307

M3 - Article

C2 - 27556857

AN - SCOPUS:84993990837

VL - 7

SP - 62939

EP - 62953

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 39

ER -