Systematic deletion of the ER lectin chaperone genes reveals their roles in vegetative growth and male gametophyte development in Arabidopsis

Kien Van Vu, Ngoc Trinh Nguyen, Chan Young Jeong, Yong Hwa Lee, Hojoung Lee, Suk Whan Hong

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Calnexin (CNX) and calreticulin (CRT) are homologous lectin chaperones in the endoplasmic reticulum (ER) that facilitate glycoprotein folding and retain folding intermediates to prevent their transit via the secretary pathway. The Arabidopsis genome has two CNX (CNX1 and CNX2) and three CRT (CRT1, CRT2 and CRT3) homologs. Despite growing evidence of the biological roles of CNXs and CRTs, little is understood about their function in Arabidopsis growth and development under normal conditions. Here, we report that the deletion of CNX1, but not of CNX2, in the crt1 crt2 crt3 triple mutation background had an adverse effect on pollen viability and pollen tube growth, leading to a significant reduction in fertility. The cnx1 crt1 crt2 crt3 quadruple mutation also conferred severe defects in growth and development, including a shortened primary root, increased root hair length and density, and reduced plant height. Disruption of all five members of the CNX/CRT family was revealed to be lethal. Finally, the abnormal phenotype of the cnx1 crt1 crt2 crt3 quadruple mutants was completely rescued by either the CNX1 or CNX2 cDNA under the control of the CNX1 promoter, suggesting functional redundancy between CNX1 and CNX2. Taken together, these results provide genetic evidence that CNX and CRT play essential and overlapping roles during vegetative growth and male gametophyte development in Arabidopsis.

Original languageEnglish
Pages (from-to)972-983
Number of pages12
JournalPlant Journal
Volume89
Issue number5
DOIs
Publication statusPublished - 2017 Mar 1

Fingerprint

calnexin
Calnexin
calreticulin
Calreticulin
Arabidopsis
Lectins
Endoplasmic Reticulum
lectins
endoplasmic reticulum
vegetative growth
Growth
Growth and Development
Genes
growth and development
genes
Pollen Tube
mutation
Mutation
root hairs
Pollen

Keywords

  • Arabidopsis
  • CNX/CRT cycle
  • endoplasmic reticulum
  • protein folding
  • quality control
  • secretory pathway

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Systematic deletion of the ER lectin chaperone genes reveals their roles in vegetative growth and male gametophyte development in Arabidopsis. / Vu, Kien Van; Nguyen, Ngoc Trinh; Jeong, Chan Young; Lee, Yong Hwa; Lee, Hojoung; Hong, Suk Whan.

In: Plant Journal, Vol. 89, No. 5, 01.03.2017, p. 972-983.

Research output: Contribution to journalArticle

Vu, Kien Van ; Nguyen, Ngoc Trinh ; Jeong, Chan Young ; Lee, Yong Hwa ; Lee, Hojoung ; Hong, Suk Whan. / Systematic deletion of the ER lectin chaperone genes reveals their roles in vegetative growth and male gametophyte development in Arabidopsis. In: Plant Journal. 2017 ; Vol. 89, No. 5. pp. 972-983.
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