Abstract
Calnexin (CNX) and calreticulin (CRT) are homologous lectin chaperones in the endoplasmic reticulum (ER) that facilitate glycoprotein folding and retain folding intermediates to prevent their transit via the secretary pathway. The Arabidopsis genome has two CNX (CNX1 and CNX2) and three CRT (CRT1, CRT2 and CRT3) homologs. Despite growing evidence of the biological roles of CNXs and CRTs, little is understood about their function in Arabidopsis growth and development under normal conditions. Here, we report that the deletion of CNX1, but not of CNX2, in the crt1 crt2 crt3 triple mutation background had an adverse effect on pollen viability and pollen tube growth, leading to a significant reduction in fertility. The cnx1 crt1 crt2 crt3 quadruple mutation also conferred severe defects in growth and development, including a shortened primary root, increased root hair length and density, and reduced plant height. Disruption of all five members of the CNX/CRT family was revealed to be lethal. Finally, the abnormal phenotype of the cnx1 crt1 crt2 crt3 quadruple mutants was completely rescued by either the CNX1 or CNX2 cDNA under the control of the CNX1 promoter, suggesting functional redundancy between CNX1 and CNX2. Taken together, these results provide genetic evidence that CNX and CRT play essential and overlapping roles during vegetative growth and male gametophyte development in Arabidopsis.
| Original language | English |
|---|---|
| Pages (from-to) | 972-983 |
| Number of pages | 12 |
| Journal | Plant Journal |
| Volume | 89 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2017 Mar 1 |
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Keywords
- Arabidopsis
- CNX/CRT cycle
- endoplasmic reticulum
- protein folding
- quality control
- secretory pathway
ASJC Scopus subject areas
- Genetics
- Plant Science
- Cell Biology
Cite this
Systematic deletion of the ER lectin chaperone genes reveals their roles in vegetative growth and male gametophyte development in Arabidopsis. / Vu, Kien Van; Nguyen, Ngoc Trinh; Jeong, Chan Young; Lee, Yong Hwa; Lee, Hojoung; Hong, Suk Whan.
In: Plant Journal, Vol. 89, No. 5, 01.03.2017, p. 972-983.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Systematic deletion of the ER lectin chaperone genes reveals their roles in vegetative growth and male gametophyte development in Arabidopsis
AU - Vu, Kien Van
AU - Nguyen, Ngoc Trinh
AU - Jeong, Chan Young
AU - Lee, Yong Hwa
AU - Lee, Hojoung
AU - Hong, Suk Whan
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Calnexin (CNX) and calreticulin (CRT) are homologous lectin chaperones in the endoplasmic reticulum (ER) that facilitate glycoprotein folding and retain folding intermediates to prevent their transit via the secretary pathway. The Arabidopsis genome has two CNX (CNX1 and CNX2) and three CRT (CRT1, CRT2 and CRT3) homologs. Despite growing evidence of the biological roles of CNXs and CRTs, little is understood about their function in Arabidopsis growth and development under normal conditions. Here, we report that the deletion of CNX1, but not of CNX2, in the crt1 crt2 crt3 triple mutation background had an adverse effect on pollen viability and pollen tube growth, leading to a significant reduction in fertility. The cnx1 crt1 crt2 crt3 quadruple mutation also conferred severe defects in growth and development, including a shortened primary root, increased root hair length and density, and reduced plant height. Disruption of all five members of the CNX/CRT family was revealed to be lethal. Finally, the abnormal phenotype of the cnx1 crt1 crt2 crt3 quadruple mutants was completely rescued by either the CNX1 or CNX2 cDNA under the control of the CNX1 promoter, suggesting functional redundancy between CNX1 and CNX2. Taken together, these results provide genetic evidence that CNX and CRT play essential and overlapping roles during vegetative growth and male gametophyte development in Arabidopsis.
AB - Calnexin (CNX) and calreticulin (CRT) are homologous lectin chaperones in the endoplasmic reticulum (ER) that facilitate glycoprotein folding and retain folding intermediates to prevent their transit via the secretary pathway. The Arabidopsis genome has two CNX (CNX1 and CNX2) and three CRT (CRT1, CRT2 and CRT3) homologs. Despite growing evidence of the biological roles of CNXs and CRTs, little is understood about their function in Arabidopsis growth and development under normal conditions. Here, we report that the deletion of CNX1, but not of CNX2, in the crt1 crt2 crt3 triple mutation background had an adverse effect on pollen viability and pollen tube growth, leading to a significant reduction in fertility. The cnx1 crt1 crt2 crt3 quadruple mutation also conferred severe defects in growth and development, including a shortened primary root, increased root hair length and density, and reduced plant height. Disruption of all five members of the CNX/CRT family was revealed to be lethal. Finally, the abnormal phenotype of the cnx1 crt1 crt2 crt3 quadruple mutants was completely rescued by either the CNX1 or CNX2 cDNA under the control of the CNX1 promoter, suggesting functional redundancy between CNX1 and CNX2. Taken together, these results provide genetic evidence that CNX and CRT play essential and overlapping roles during vegetative growth and male gametophyte development in Arabidopsis.
KW - Arabidopsis
KW - CNX/CRT cycle
KW - endoplasmic reticulum
KW - protein folding
KW - quality control
KW - secretory pathway
UR - http://www.scopus.com/inward/record.url?scp=85013176288&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85013176288&partnerID=8YFLogxK
U2 - 10.1111/tpj.13435
DO - 10.1111/tpj.13435
M3 - Article
C2 - 27888524
AN - SCOPUS:85013176288
VL - 89
SP - 972
EP - 983
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 5
ER -