T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity via αvβ3 and α5β1 integrins

Ju Ock Nam, Mi Yeon Jung, Narendra Thapa, Byung Heon Lee, Rang Woon Park, In-San Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We made fusion protein of fastatin and FIII 9-10, termed tetra-cell adhesion molecule (T-CAM) that can interact simultaneously with αvβ3 and α5β1 integrins, both playing important roles in tumor angiogenesis. T-CAM can serve as a cell adhesion substrate mediating adhesion and migration of endothelial cells in αvβ3 and α5β1 integrin-dependent manner. T-CAM showed pronounced anti-angiogenic activities such as inhibition of endothelial cell tube formation, endothelial cell proliferation, and induction of endothelial cell apoptosis. T-CAM also inhibited angiogenesis and tumor growth in mouse xenograft model. The anti-angiogenic and anti-tumoral activity of molecule like fastatin could be improved by fusing it with integrin-recognizing cell adhesion domain from other distinct proteins. The strategy of combining two distinct anti-angiogenic molecules or cell adhesion domains could facilitate designing improved anticancer agent of therapeutic value.

Original languageEnglish
Pages (from-to)196-207
Number of pages12
JournalExperimental and Molecular Medicine
Volume40
Issue number2
DOIs
Publication statusPublished - 2008 Apr 30
Externally publishedYes

Fingerprint

Endothelial cells
Cell Adhesion Molecules
Integrins
Tumors
Cell adhesion
Fusion reactions
Endothelial Cells
Neoplasms
Proteins
Cell Adhesion
Molecules
Cell proliferation
Heterografts
Antineoplastic Agents
Adhesion
Apoptosis
Cell Proliferation
Substrates
Growth

Keywords

  • Angiogenesis inhibitors
  • Angiostatic proteins
  • Antineoplastic agents
  • Cell adhesion molecules
  • Integrin α5β1
  • Integrin αvβ3

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity via αvβ3 and α5β1 integrins. / Nam, Ju Ock; Jung, Mi Yeon; Thapa, Narendra; Lee, Byung Heon; Park, Rang Woon; Kim, In-San.

In: Experimental and Molecular Medicine, Vol. 40, No. 2, 30.04.2008, p. 196-207.

Research output: Contribution to journalArticle

Nam, Ju Ock ; Jung, Mi Yeon ; Thapa, Narendra ; Lee, Byung Heon ; Park, Rang Woon ; Kim, In-San. / T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity via αvβ3 and α5β1 integrins. In: Experimental and Molecular Medicine. 2008 ; Vol. 40, No. 2. pp. 196-207.
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