T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity via αvβ3 and α5β1 integrins

Ju Ock Nam, Mi Yeon Jung, Narendra Thapa, Byung Heon Lee, Rang Woon Park, In-San Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We made fusion protein of fastatin and FIII 9-10, termed tetra-cell adhesion molecule (T-CAM) that can interact simultaneously with αvβ3 and α5β1 integrins, both playing important roles in tumor angiogenesis. T-CAM can serve as a cell adhesion substrate mediating adhesion and migration of endothelial cells in αvβ3 and α5β1 integrin-dependent manner. T-CAM showed pronounced anti-angiogenic activities such as inhibition of endothelial cell tube formation, endothelial cell proliferation, and induction of endothelial cell apoptosis. T-CAM also inhibited angiogenesis and tumor growth in mouse xenograft model. The anti-angiogenic and anti-tumoral activity of molecule like fastatin could be improved by fusing it with integrin-recognizing cell adhesion domain from other distinct proteins. The strategy of combining two distinct anti-angiogenic molecules or cell adhesion domains could facilitate designing improved anticancer agent of therapeutic value.

Original languageEnglish
Pages (from-to)196-207
Number of pages12
JournalExperimental and Molecular Medicine
Volume40
Issue number2
DOIs
Publication statusPublished - 2008 Apr 30
Externally publishedYes

    Fingerprint

Keywords

  • Angiogenesis inhibitors
  • Angiostatic proteins
  • Antineoplastic agents
  • Cell adhesion molecules
  • Integrin α5β1
  • Integrin αvβ3

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this