Abstract
Cerebral ischemia resulting from transient or permanent cerebral artery occlusion leads to neuronal cell death, and eventually causes neurological impairments. Tadalafil (Cialis®) is a long-acting phosphodiesterase type-5 (PDE-5) inhibitor used to treat erectile dysfunction. The therapeutic effects of PDE-5 inhibitors on chronic obstructive pulmonary disease, prostate hyperplasia, hypertension, and coronary heart disease have been reported. The present study investigated the effects of tadalafil on short-term memory, cyclic guanosine monophosphate (cGMP) level, apoptotic neuronal cell death, and cell proliferation in the hippocampus following transient global ischemia in gerbils. For this study, a step-down avoidance task, cGMP assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, and immunohistochemistry for caspase-3 and 5-bromo-2′-deoxyuridine were performed. The results revealed that ischemic injury increased apoptotic neuronal cell death in the hippocampal CA1 region, impaired short-term memory, and decreased cGMP level. Ischemic injury enhanced cell proliferation in the hippocampal dentate gyrus. Tadalafil treatment improved short-term memory by suppressing ischemia-induced apoptotic neuronal cell death in the hippocampal CA1 region, and decreased cGMP level. Also, tadalafil suppressed the ischemia-induced increase in cell proliferation in the hippocampal dentate gyrus. We showed that tadalafil can overcome ischemia-induced apoptotic neuronal cell death, thus facilitates recovery following ischemic cerebral injury.
| Original language | English |
|---|---|
| Pages (from-to) | 629-635 |
| Number of pages | 7 |
| Journal | Pharmacology Biochemistry and Behavior |
| Volume | 91 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2009 Feb 1 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Cell proliferation
- Cerebral ischemia
- cGMP level
- Phosphodiesterase-5 inhibitor
- Short-term memory
- Tadalafil
ASJC Scopus subject areas
- Biochemistry
- Toxicology
- Pharmacology
- Clinical Biochemistry
- Biological Psychiatry
- Behavioral Neuroscience
Cite this
Tadalafil improves short-term memory by suppressing ischemia-induced apoptosis of hippocampal neuronal cells in gerbils. / Ko, Il Gyu; Shin, Mal Soon; Kim, Bo Kyun; Kim, Sung Eun; Sung, Yun Hee; Kim, Tae Soo; Shin, Min Chul; Cho, Han Jin; Kim, Sin Chul; Kim, Sang Hoon; Kim, Khae Hawn; Shin, Dong Hoon; Kim, Chang Ju.
In: Pharmacology Biochemistry and Behavior, Vol. 91, No. 4, 01.02.2009, p. 629-635.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tadalafil improves short-term memory by suppressing ischemia-induced apoptosis of hippocampal neuronal cells in gerbils
AU - Ko, Il Gyu
AU - Shin, Mal Soon
AU - Kim, Bo Kyun
AU - Kim, Sung Eun
AU - Sung, Yun Hee
AU - Kim, Tae Soo
AU - Shin, Min Chul
AU - Cho, Han Jin
AU - Kim, Sin Chul
AU - Kim, Sang Hoon
AU - Kim, Khae Hawn
AU - Shin, Dong Hoon
AU - Kim, Chang Ju
PY - 2009/2/1
Y1 - 2009/2/1
N2 - Cerebral ischemia resulting from transient or permanent cerebral artery occlusion leads to neuronal cell death, and eventually causes neurological impairments. Tadalafil (Cialis®) is a long-acting phosphodiesterase type-5 (PDE-5) inhibitor used to treat erectile dysfunction. The therapeutic effects of PDE-5 inhibitors on chronic obstructive pulmonary disease, prostate hyperplasia, hypertension, and coronary heart disease have been reported. The present study investigated the effects of tadalafil on short-term memory, cyclic guanosine monophosphate (cGMP) level, apoptotic neuronal cell death, and cell proliferation in the hippocampus following transient global ischemia in gerbils. For this study, a step-down avoidance task, cGMP assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, and immunohistochemistry for caspase-3 and 5-bromo-2′-deoxyuridine were performed. The results revealed that ischemic injury increased apoptotic neuronal cell death in the hippocampal CA1 region, impaired short-term memory, and decreased cGMP level. Ischemic injury enhanced cell proliferation in the hippocampal dentate gyrus. Tadalafil treatment improved short-term memory by suppressing ischemia-induced apoptotic neuronal cell death in the hippocampal CA1 region, and decreased cGMP level. Also, tadalafil suppressed the ischemia-induced increase in cell proliferation in the hippocampal dentate gyrus. We showed that tadalafil can overcome ischemia-induced apoptotic neuronal cell death, thus facilitates recovery following ischemic cerebral injury.
AB - Cerebral ischemia resulting from transient or permanent cerebral artery occlusion leads to neuronal cell death, and eventually causes neurological impairments. Tadalafil (Cialis®) is a long-acting phosphodiesterase type-5 (PDE-5) inhibitor used to treat erectile dysfunction. The therapeutic effects of PDE-5 inhibitors on chronic obstructive pulmonary disease, prostate hyperplasia, hypertension, and coronary heart disease have been reported. The present study investigated the effects of tadalafil on short-term memory, cyclic guanosine monophosphate (cGMP) level, apoptotic neuronal cell death, and cell proliferation in the hippocampus following transient global ischemia in gerbils. For this study, a step-down avoidance task, cGMP assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, and immunohistochemistry for caspase-3 and 5-bromo-2′-deoxyuridine were performed. The results revealed that ischemic injury increased apoptotic neuronal cell death in the hippocampal CA1 region, impaired short-term memory, and decreased cGMP level. Ischemic injury enhanced cell proliferation in the hippocampal dentate gyrus. Tadalafil treatment improved short-term memory by suppressing ischemia-induced apoptotic neuronal cell death in the hippocampal CA1 region, and decreased cGMP level. Also, tadalafil suppressed the ischemia-induced increase in cell proliferation in the hippocampal dentate gyrus. We showed that tadalafil can overcome ischemia-induced apoptotic neuronal cell death, thus facilitates recovery following ischemic cerebral injury.
KW - Apoptosis
KW - Cell proliferation
KW - Cerebral ischemia
KW - cGMP level
KW - Phosphodiesterase-5 inhibitor
KW - Short-term memory
KW - Tadalafil
UR - http://www.scopus.com/inward/record.url?scp=58749103648&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58749103648&partnerID=8YFLogxK
U2 - 10.1016/j.pbb.2008.10.009
DO - 10.1016/j.pbb.2008.10.009
M3 - Article
C2 - 19010346
AN - SCOPUS:58749103648
VL - 91
SP - 629
EP - 635
JO - Pharmacology Biochemistry and Behavior
JF - Pharmacology Biochemistry and Behavior
SN - 0091-3057
IS - 4
ER -