Tailoring the supramolecular structure of aminated polyrotaxanes toward enhanced cellular internalization

Nanako Yokoyama, Ji Hun Seo, Atsushi Tamura, Yoshihiro Sasaki, Nobuhiko Yui

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated by flow cytometry and confocal laser scanning microscopy. AF-545-labeled aminated PRXs (APRXs) containing different numbers of threaded α-cyclodextrins (CDs) and amino groups are synthesized; their cellular uptakes are analyzed using HeLa cells in serum. The APRX threaded CD number is discovered to be a more critical factor for enhancing cellular internalization than the APRX amine content. Additionally, APRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules. Because increased numbers of threaded CDs are related to increased PRX rigidity, the PRX rigid frame resulting from CD molecules threaded on a poly(ethylene glycol) (PEG) chain is suitable for intracellular tools in therapy and diagnosis. The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated. The PRX threaded α-cyclodextrin number is discovered to be a more critical factor for enhancing cellular internalization than the PRX amine content. Additionally, aminated PRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules.

Original languageEnglish
Pages (from-to)359-368
Number of pages10
JournalMacromolecular Bioscience
Volume14
Issue number3
DOIs
Publication statusPublished - 2014 Mar
Externally publishedYes

Keywords

  • cellular internalization
  • cyclodextrin
  • electrostatic interaction
  • polyrotaxane
  • supramolecular structures

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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