Tanshinone IIA exerts antitumor activity against vestibular schwannoma cells by inhibiting the expression of hypoxia-inducible factor-1α

Ju Yeon Kim, Jae Jun Song, Byoung Mog Kwon, Jong Dae Lee

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    The aim of the present study was to evaluate the effect of the herbal medicine, tanshinone IIA (Tan IIA), on vestibular schwannoma (VS) cells and assess the functional targets of Tan IIA. HEI-193 cells and Nf2-/-mouse Schwann (SC4) cells were used to investigate the inhibitory effects of Tan IIA on VS. Cell viability was measured using an MTT assay and apoptosis was assessed by flow cytometry. Western blot analysis and reverse transcription quantitative polymerase chain reaction (RT-qPCR) were performed to assess the expression of hypoxia-inducible factor-1α (HIF-1α) and its signaling pathways. In addition, the effect of Tan IIA on HIF-1α transcription was determined using a luciferase reporter assay. Schwannoma cell proliferation was observed to be inhibited as the Tan IIA concentration increased under normoxic and hypoxic conditions. Furthermore, Tan IIA induced apoptosis in the HEI-193 cells and inhibited the protein expression of HIF-1α in the HEI-193 cells under hypoxia, thus repressing the transcriptional activity of HIF-1α. The present study demonstrated that HIF-1α is expressed in hypoxic VS cells and Tan IIA inhibits VS cells by suppressing the activity of HIF-1α. In conclusion, these results indicate that Tan IIA is a potential chemotherapeutic agent for the treatment of VS.

    Original languageEnglish
    Pages (from-to)4604-4609
    Number of pages6
    JournalMolecular Medicine Reports
    Volume12
    Issue number3
    DOIs
    Publication statusPublished - 2015 Sep 1

    Keywords

    • Hypoxia-inducible factor
    • Tanshinone IIA
    • Vestibular schwannoma

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Genetics
    • Oncology
    • Cancer Research

    Fingerprint

    Dive into the research topics of 'Tanshinone IIA exerts antitumor activity against vestibular schwannoma cells by inhibiting the expression of hypoxia-inducible factor-1α'. Together they form a unique fingerprint.

    Cite this