Abstract
The aim of the present study was to evaluate the effect of the herbal medicine, tanshinone IIA (Tan IIA), on vestibular schwannoma (VS) cells and assess the functional targets of Tan IIA. HEI-193 cells and Nf2-/-mouse Schwann (SC4) cells were used to investigate the inhibitory effects of Tan IIA on VS. Cell viability was measured using an MTT assay and apoptosis was assessed by flow cytometry. Western blot analysis and reverse transcription quantitative polymerase chain reaction (RT-qPCR) were performed to assess the expression of hypoxia-inducible factor-1α (HIF-1α) and its signaling pathways. In addition, the effect of Tan IIA on HIF-1α transcription was determined using a luciferase reporter assay. Schwannoma cell proliferation was observed to be inhibited as the Tan IIA concentration increased under normoxic and hypoxic conditions. Furthermore, Tan IIA induced apoptosis in the HEI-193 cells and inhibited the protein expression of HIF-1α in the HEI-193 cells under hypoxia, thus repressing the transcriptional activity of HIF-1α. The present study demonstrated that HIF-1α is expressed in hypoxic VS cells and Tan IIA inhibits VS cells by suppressing the activity of HIF-1α. In conclusion, these results indicate that Tan IIA is a potential chemotherapeutic agent for the treatment of VS.
| Original language | English |
|---|---|
| Pages (from-to) | 4604-4609 |
| Number of pages | 6 |
| Journal | Molecular Medicine Reports |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2015 Jan 1 |
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Keywords
- Hypoxia-inducible factor
- Tanshinone IIA
- Vestibular schwannoma
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Oncology
- Genetics
- Cancer Research
Cite this
Tanshinone IIA exerts antitumor activity against vestibular schwannoma cells by inhibiting the expression of hypoxia-inducible factor-1α. / Kim, Ju Yeon; Song, Jae-Jun; Kwon, Byoung Mog; Lee, Jong Dae.
In: Molecular Medicine Reports, Vol. 12, No. 3, 01.01.2015, p. 4604-4609.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tanshinone IIA exerts antitumor activity against vestibular schwannoma cells by inhibiting the expression of hypoxia-inducible factor-1α
AU - Kim, Ju Yeon
AU - Song, Jae-Jun
AU - Kwon, Byoung Mog
AU - Lee, Jong Dae
PY - 2015/1/1
Y1 - 2015/1/1
N2 - The aim of the present study was to evaluate the effect of the herbal medicine, tanshinone IIA (Tan IIA), on vestibular schwannoma (VS) cells and assess the functional targets of Tan IIA. HEI-193 cells and Nf2-/-mouse Schwann (SC4) cells were used to investigate the inhibitory effects of Tan IIA on VS. Cell viability was measured using an MTT assay and apoptosis was assessed by flow cytometry. Western blot analysis and reverse transcription quantitative polymerase chain reaction (RT-qPCR) were performed to assess the expression of hypoxia-inducible factor-1α (HIF-1α) and its signaling pathways. In addition, the effect of Tan IIA on HIF-1α transcription was determined using a luciferase reporter assay. Schwannoma cell proliferation was observed to be inhibited as the Tan IIA concentration increased under normoxic and hypoxic conditions. Furthermore, Tan IIA induced apoptosis in the HEI-193 cells and inhibited the protein expression of HIF-1α in the HEI-193 cells under hypoxia, thus repressing the transcriptional activity of HIF-1α. The present study demonstrated that HIF-1α is expressed in hypoxic VS cells and Tan IIA inhibits VS cells by suppressing the activity of HIF-1α. In conclusion, these results indicate that Tan IIA is a potential chemotherapeutic agent for the treatment of VS.
AB - The aim of the present study was to evaluate the effect of the herbal medicine, tanshinone IIA (Tan IIA), on vestibular schwannoma (VS) cells and assess the functional targets of Tan IIA. HEI-193 cells and Nf2-/-mouse Schwann (SC4) cells were used to investigate the inhibitory effects of Tan IIA on VS. Cell viability was measured using an MTT assay and apoptosis was assessed by flow cytometry. Western blot analysis and reverse transcription quantitative polymerase chain reaction (RT-qPCR) were performed to assess the expression of hypoxia-inducible factor-1α (HIF-1α) and its signaling pathways. In addition, the effect of Tan IIA on HIF-1α transcription was determined using a luciferase reporter assay. Schwannoma cell proliferation was observed to be inhibited as the Tan IIA concentration increased under normoxic and hypoxic conditions. Furthermore, Tan IIA induced apoptosis in the HEI-193 cells and inhibited the protein expression of HIF-1α in the HEI-193 cells under hypoxia, thus repressing the transcriptional activity of HIF-1α. The present study demonstrated that HIF-1α is expressed in hypoxic VS cells and Tan IIA inhibits VS cells by suppressing the activity of HIF-1α. In conclusion, these results indicate that Tan IIA is a potential chemotherapeutic agent for the treatment of VS.
KW - Hypoxia-inducible factor
KW - Tanshinone IIA
KW - Vestibular schwannoma
UR - http://www.scopus.com/inward/record.url?scp=84935454896&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84935454896&partnerID=8YFLogxK
U2 - 10.3892/mmr.2015.3932
DO - 10.3892/mmr.2015.3932
M3 - Article
C2 - 26080622
AN - SCOPUS:84935454896
VL - 12
SP - 4604
EP - 4609
JO - Molecular Medicine Reports
JF - Molecular Medicine Reports
SN - 1791-2997
IS - 3
ER -