Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production

Ye Sol Shin, Jooyeon Jeong, Thu Ha Thi Nguyen, Jaoon Young Hwan Kim, Eon Seon Jin, Sang Jun Sim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A2 knockout mutants have increased by up to 64.25% (to 80.92 g L−1 d−1). This study can provide crucial information for the biodiesel industry.

Original languageEnglish
Pages (from-to)368-374
Number of pages7
JournalBioresource Technology
Volume271
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Biofuels
Phospholipases A2
Biodiesel
Lipids
Clustered Regularly Interspaced Short Palindromic Repeats
Genes
Productivity
lipid
productivity
gene
Diglycerides
Cell growth
biofuel
Crops
Triglycerides
Enzymes
enzyme
crop
industry
cost

Keywords

  • Biofuel
  • Chlamydomonas reinhardtii
  • CRISPR-CAS9
  • Phospholipase
  • Triacylglycerol

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production. / Shin, Ye Sol; Jeong, Jooyeon; Nguyen, Thu Ha Thi; Kim, Jaoon Young Hwan; Jin, Eon Seon; Sim, Sang Jun.

In: Bioresource Technology, Vol. 271, 01.01.2019, p. 368-374.

Research output: Contribution to journalArticle

Shin, Ye Sol ; Jeong, Jooyeon ; Nguyen, Thu Ha Thi ; Kim, Jaoon Young Hwan ; Jin, Eon Seon ; Sim, Sang Jun. / Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production. In: Bioresource Technology. 2019 ; Vol. 271. pp. 368-374.
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