Abstract
Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A2 knockout mutants have increased by up to 64.25% (to 80.92 g L−1 d−1). This study can provide crucial information for the biodiesel industry.
| Original language | English |
|---|---|
| Pages (from-to) | 368-374 |
| Number of pages | 7 |
| Journal | Bioresource Technology |
| Volume | 271 |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
Fingerprint
Keywords
- Biofuel
- Chlamydomonas reinhardtii
- CRISPR-CAS9
- Phospholipase
- Triacylglycerol
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal
Cite this
Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production. / Shin, Ye Sol; Jeong, Jooyeon; Nguyen, Thu Ha Thi; Kim, Jaoon Young Hwan; Jin, Eon Seon; Sim, Sang Jun.
In: Bioresource Technology, Vol. 271, 01.01.2019, p. 368-374.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production
AU - Shin, Ye Sol
AU - Jeong, Jooyeon
AU - Nguyen, Thu Ha Thi
AU - Kim, Jaoon Young Hwan
AU - Jin, Eon Seon
AU - Sim, Sang Jun
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A2 knockout mutants have increased by up to 64.25% (to 80.92 g L−1 d−1). This study can provide crucial information for the biodiesel industry.
AB - Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A2 knockout mutants have increased by up to 64.25% (to 80.92 g L−1 d−1). This study can provide crucial information for the biodiesel industry.
KW - Biofuel
KW - Chlamydomonas reinhardtii
KW - CRISPR-CAS9
KW - Phospholipase
KW - Triacylglycerol
UR - http://www.scopus.com/inward/record.url?scp=85054180320&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054180320&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2018.09.121
DO - 10.1016/j.biortech.2018.09.121
M3 - Article
C2 - 30293032
AN - SCOPUS:85054180320
VL - 271
SP - 368
EP - 374
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -