Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production

Ye Sol Shin; Jooyeon Jeong; Thu Ha Thi Nguyen; Jaoon Young Hwan Kim; Eon Seon Jin; Sang Jun Sim

doi:10.1016/j.biortech.2018.09.121

Targeted knockout of phospholipase A₂ to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production

Ye Sol Shin, Jooyeon Jeong, Thu Ha Thi Nguyen, Jaoon Young Hwan Kim, Eon Seon Jin, Sang Jun Sim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A₂ gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A₂ knockout mutants have increased by up to 64.25% (to 80.92 g L⁻¹ d⁻¹). This study can provide crucial information for the biodiesel industry.

Original language	English
Pages (from-to)	368-374
Number of pages	7
Journal	Bioresource technology
Volume	271
DOIs	https://doi.org/10.1016/j.biortech.2018.09.121
Publication status	Published - 2019 Jan

Keywords

Biofuel
CRISPR-CAS9
Chlamydomonas reinhardtii
Phospholipase
Triacylglycerol

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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title = "Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production",

abstract = "Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A2 knockout mutants have increased by up to 64.25% (to 80.92 g L−1 d−1). This study can provide crucial information for the biodiesel industry.",

keywords = "Biofuel, CRISPR-CAS9, Chlamydomonas reinhardtii, Phospholipase, Triacylglycerol",

author = "Shin, {Ye Sol} and Jooyeon Jeong and Nguyen, {Thu Ha Thi} and Kim, {Jaoon Young Hwan} and Jin, {Eon Seon} and Sim, {Sang Jun}",

note = "Funding Information: The authors would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278) and National Research Foundation of Korea (NRF) funded by the Korea government (grant number: NRF-2016R1A2A1A05005465 ) and Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government ( Ministry of Trade, Industry and Energy ) (grant number: 20172010202050 ). Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2019",

month = jan,

doi = "10.1016/j.biortech.2018.09.121",

language = "English",

volume = "271",

pages = "368--374",

journal = "Bioresource Technology",

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AU - Shin, Ye Sol

AU - Jeong, Jooyeon

AU - Nguyen, Thu Ha Thi

AU - Kim, Jaoon Young Hwan

AU - Jin, Eon Seon

AU - Sim, Sang Jun

N1 - Funding Information: The authors would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government (Ministry of Science and ICT) in 2017 (grant number: KCRC-2014M1A8A1049278) and National Research Foundation of Korea (NRF) funded by the Korea government (grant number: NRF-2016R1A2A1A05005465 ) and Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government ( Ministry of Trade, Industry and Energy ) (grant number: 20172010202050 ). Publisher Copyright: © 2018 Elsevier Ltd

PY - 2019/1

Y1 - 2019/1

N2 - Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A2 knockout mutants have increased by up to 64.25% (to 80.92 g L−1 d−1). This study can provide crucial information for the biodiesel industry.

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