Targeting of a multicomponent transcription apparatus into assembling vaccinia virus particles requires RAP94, an RNA polymerase-associated protein

Yifan Zhang, Byung Yoon Ahn, Bernard Moss

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

When expression of the vaccinia virus gene encoding RAP94 (a protein that is associated with the viral multisubunit RNA polymerase and confers transcriptional specificity for early promoters) was repressed, the infectious virus yield was reduced by more than 99%. Nevertheless, intermediate- and late-stage viral gene expression and formation of ultrastructurally mature, membrane-enveloped virions occurred under the nonpermissive conditions. The RAP94-deficient particles contained the viral genome, structural proteins, early transcription factor, and certain enzymes but, unlike normal virions, had low or undetectable amounts of the viral RNA polymerase, capping enzyme/termination factor, poly(A) polymerase, DNA- dependent ATPase, RNA helicase, and topoisomerase. The presence of these viral enzymes in the cytoplasm indicated that RAP94 is required for targeting a complex of functionally related proteins involved in the biosynthesis of mRNA.

Original languageEnglish
Pages (from-to)1360-1370
Number of pages11
JournalJournal of virology
Volume68
Issue number3
DOIs
Publication statusPublished - 1994 Mar

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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