Targeting of a multicomponent transcription apparatus into assembling vaccinia virus particles requires RAP94, an RNA polymerase-associated protein

Yifan Zhang, Byung-Yoon Ahn, Bernard Moss

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

When expression of the vaccinia virus gene encoding RAP94 (a protein that is associated with the viral multisubunit RNA polymerase and confers transcriptional specificity for early promoters) was repressed, the infectious virus yield was reduced by more than 99%. Nevertheless, intermediate- and late-stage viral gene expression and formation of ultrastructurally mature, membrane-enveloped virions occurred under the nonpermissive conditions. The RAP94-deficient particles contained the viral genome, structural proteins, early transcription factor, and certain enzymes but, unlike normal virions, had low or undetectable amounts of the viral RNA polymerase, capping enzyme/termination factor, poly(A) polymerase, DNA- dependent ATPase, RNA helicase, and topoisomerase. The presence of these viral enzymes in the cytoplasm indicated that RAP94 is required for targeting a complex of functionally related proteins involved in the biosynthesis of mRNA.

Original languageEnglish
Pages (from-to)1360-1370
Number of pages11
JournalJournal of Virology
Volume68
Issue number3
Publication statusPublished - 1994 Mar 1

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Vaccinia virus
DNA-Directed RNA Polymerases
Virion
Viral RNA
Enzymes
Polynucleotide Adenylyltransferase
RNA Helicases
Viral Structural Proteins
Proteins
Viral Genes
Viral Genome
Adenosine Triphosphatases
Cytoplasm
Transcription Factors
Viruses
Gene Expression
Messenger RNA
Membranes
Genes

ASJC Scopus subject areas

  • Immunology

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Targeting of a multicomponent transcription apparatus into assembling vaccinia virus particles requires RAP94, an RNA polymerase-associated protein. / Zhang, Yifan; Ahn, Byung-Yoon; Moss, Bernard.

In: Journal of Virology, Vol. 68, No. 3, 01.03.1994, p. 1360-1370.

Research output: Contribution to journalArticle

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