Taurine is a liver X receptor-α ligand and activates transcription of key genes in the reverse cholesterol transport without inducing hepatic lipogenesis

Minh Hien Hoang, Yaoyao Jia, Hee jin Jun, Ji Hae Lee, Kwang Yeon Hwang, Dal Woong Choi, Soo Jong Um, Boo Yong Lee, Sang Guan You, Sung Joon Lee

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Scope: Taurine, which is abundant in seafood, has antiatherogenic activities in both animals and humans; however, its molecular target has been elusive. We examined whether taurine could activate liver X receptor-α (LXR-α), a critical transcription factor in the regulation of reverse cholesterol transport in macrophages. Methods and results: Taurine bound directly to LXR-α in a reporter gene assay, time-resolved fluorescence resonance energy transfer analysis, and limited protease digestion experiment. Macrophage cells incubated with taurine showed reduced cellular cholesterol and induced medium cholesterol in a dose-dependent manner with the induction of ATP-binding cassette transporter A1 and G gene and protein expression. In hepatocytes, taurine significantly induced Insig-2a levels and delayed nuclear translocation of the sterol regulatory element-binding protein 1 (SREBP-1) protein, resulting in a dose-dependent reduction in the cellular lipid levels without inducing the expression of fatty acid synthesis genes. Conclusion: Taurine is a direct LXR-α ligand, represses cholesterol accumulation, and modulates the expression of genes involved in reverse cholesterol transport in macrophages, without inducing hepatic lipogenesis. The induction of Insig-2a suppressed the nuclear translocation of SREBP-1c.

Original languageEnglish
Pages (from-to)900-911
Number of pages12
JournalMolecular Nutrition and Food Research
Volume56
Issue number6
DOIs
Publication statusPublished - 2012 Jun

Keywords

  • Cholesterol
  • Insig-2a
  • LXR-α
  • SREBP-1c
  • Taurine

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

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