Taurine is a liver X receptor-α ligand and activates transcription of key genes in the reverse cholesterol transport without inducing hepatic lipogenesis

Minh Hien Hoang, Yaoyao Jia, Hee jin Jun, Ji Hae Lee, Kwang Yeon Hwang, DalWoong Choi, Soo Jong Um, Boo Yong Lee, Sang Guan You, Sung-Joon Lee

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Scope: Taurine, which is abundant in seafood, has antiatherogenic activities in both animals and humans; however, its molecular target has been elusive. We examined whether taurine could activate liver X receptor-α (LXR-α), a critical transcription factor in the regulation of reverse cholesterol transport in macrophages. Methods and results: Taurine bound directly to LXR-α in a reporter gene assay, time-resolved fluorescence resonance energy transfer analysis, and limited protease digestion experiment. Macrophage cells incubated with taurine showed reduced cellular cholesterol and induced medium cholesterol in a dose-dependent manner with the induction of ATP-binding cassette transporter A1 and G gene and protein expression. In hepatocytes, taurine significantly induced Insig-2a levels and delayed nuclear translocation of the sterol regulatory element-binding protein 1 (SREBP-1) protein, resulting in a dose-dependent reduction in the cellular lipid levels without inducing the expression of fatty acid synthesis genes. Conclusion: Taurine is a direct LXR-α ligand, represses cholesterol accumulation, and modulates the expression of genes involved in reverse cholesterol transport in macrophages, without inducing hepatic lipogenesis. The induction of Insig-2a suppressed the nuclear translocation of SREBP-1c.

Original languageEnglish
Pages (from-to)900-911
Number of pages12
JournalMolecular Nutrition and Food Research
Volume56
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1

Fingerprint

Lipogenesis
Taurine
taurine
lipogenesis
transcription (genetics)
Cholesterol
cholesterol
Ligands
liver
receptors
Liver
Sterol Regulatory Element Binding Protein 1
Genes
macrophages
genes
Macrophages
Gene Expression
Seafood
gene expression
Fluorescence Resonance Energy Transfer

Keywords

  • Cholesterol
  • Insig-2a
  • LXR-α
  • SREBP-1c
  • Taurine

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Taurine is a liver X receptor-α ligand and activates transcription of key genes in the reverse cholesterol transport without inducing hepatic lipogenesis. / Hoang, Minh Hien; Jia, Yaoyao; Jun, Hee jin; Lee, Ji Hae; Hwang, Kwang Yeon; Choi, DalWoong; Um, Soo Jong; Lee, Boo Yong; You, Sang Guan; Lee, Sung-Joon.

In: Molecular Nutrition and Food Research, Vol. 56, No. 6, 01.06.2012, p. 900-911.

Research output: Contribution to journalArticle

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AU - Jun, Hee jin

AU - Lee, Ji Hae

AU - Hwang, Kwang Yeon

AU - Choi, DalWoong

AU - Um, Soo Jong

AU - Lee, Boo Yong

AU - You, Sang Guan

AU - Lee, Sung-Joon

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