TAZ as a novel enhancer of MyoD-mediated myogenic differentiation

Hana Jeong, Sujung Bae, Su Yeon An, Mi Ran Byun, Jun Ha Hwang, Michael B. Yaffe, Jeong-Ho Hong, Eun Sook Hwang

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Myoblast differentiation is indispensable for skeletal muscle formation and is governed by the precisely coordinated regulation of a series of transcription factors, including MyoD and myogenin, and transcriptional coregulators. TAZ (transcriptional coactivator with PDZ-binding motif) has been characterized as a modulator of mesenchymal stem cell differentiation into osteoblasts and adipocytes through its regulation of lineage-specific master transcription factors. In this study, we investigated whether TAZ affects myoblast differentiation, which is one of the differentiated lineages of mesenchymal stem cells. Ectopic overexpression of TAZ in myoblasts increases myogenic gene expression in a MyoD-dependent manner and hastens myofiber formation, whereas TAZ knockdown delays myogenic differentiation. In addition, enforced coexpression of TAZ and MyoD in fibroblasts accelerates MyoD-induced myogenic differentiation. TAZ physically interacts with MyoD through the WW domain and activates MyoD-dependent gene transcription. TAZ additionally enhances the interaction of MyoD with the myogenin gene promoter. These results strongly suggest that TAZ functions as a novel transcriptional modulator of myogenic differentiation by promoting MyoD-mediated myogenic gene expression.

Original languageEnglish
Pages (from-to)3310-3320
Number of pages11
JournalFASEB Journal
Volume24
Issue number9
DOIs
Publication statusPublished - 2010 Sep 1

Fingerprint

Myogenin
Myoblasts
Stem cells
Gene expression
Modulators
Transcription Factors
Genes
Mesenchymal Stromal Cells
Osteoblasts
Transcription
Fibroblasts
Muscle
Gene Expression
Adipocytes
Cell Differentiation
Skeletal Muscle

Keywords

  • MCK
  • Mesenchymal stem cells
  • Myoblast
  • Myogenin

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Jeong, H., Bae, S., An, S. Y., Byun, M. R., Hwang, J. H., Yaffe, M. B., ... Hwang, E. S. (2010). TAZ as a novel enhancer of MyoD-mediated myogenic differentiation. FASEB Journal, 24(9), 3310-3320. https://doi.org/10.1096/fj.09-151324

TAZ as a novel enhancer of MyoD-mediated myogenic differentiation. / Jeong, Hana; Bae, Sujung; An, Su Yeon; Byun, Mi Ran; Hwang, Jun Ha; Yaffe, Michael B.; Hong, Jeong-Ho; Hwang, Eun Sook.

In: FASEB Journal, Vol. 24, No. 9, 01.09.2010, p. 3310-3320.

Research output: Contribution to journalArticle

Jeong, H, Bae, S, An, SY, Byun, MR, Hwang, JH, Yaffe, MB, Hong, J-H & Hwang, ES 2010, 'TAZ as a novel enhancer of MyoD-mediated myogenic differentiation', FASEB Journal, vol. 24, no. 9, pp. 3310-3320. https://doi.org/10.1096/fj.09-151324
Jeong H, Bae S, An SY, Byun MR, Hwang JH, Yaffe MB et al. TAZ as a novel enhancer of MyoD-mediated myogenic differentiation. FASEB Journal. 2010 Sep 1;24(9):3310-3320. https://doi.org/10.1096/fj.09-151324
Jeong, Hana ; Bae, Sujung ; An, Su Yeon ; Byun, Mi Ran ; Hwang, Jun Ha ; Yaffe, Michael B. ; Hong, Jeong-Ho ; Hwang, Eun Sook. / TAZ as a novel enhancer of MyoD-mediated myogenic differentiation. In: FASEB Journal. 2010 ; Vol. 24, No. 9. pp. 3310-3320.
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