Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

Jin Won Park; Dong June Ahn

doi:10.1016/j.colsurfb.2007.09.020

Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

Jin Won Park, Dong June Ahn

KU-KIST Graduate School of Converging Science and Technology

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.

Original language	English
Pages (from-to)	157-161
Number of pages	5
Journal	Colloids and Surfaces B: Biointerfaces
Volume	62
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfb.2007.09.020
Publication status	Published - 2008 Mar 15

Keywords

Adhesion
Atomic force microscopy
Deformation elasticity
Jump-to-surface
Langmuir-Blodgett technique
Phase-separation

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2007.09.020

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title = "Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers",

abstract = "Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.",

keywords = "Adhesion, Atomic force microscopy, Deformation elasticity, Jump-to-surface, Langmuir-Blodgett technique, Phase-separation",

author = "Park, {Jin Won} and Ahn, {Dong June}",

note = "Funding Information: This research was supported by Gachon Bio-Nano Research Institute, Kyungwon University. We thank Prof. I.S. Yoo at the Kyungwon University and Dr. G.S. Lee at the Korea University for valuable discussions.",

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doi = "10.1016/j.colsurfb.2007.09.020",

language = "English",

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T1 - Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

AU - Park, Jin Won

AU - Ahn, Dong June

N1 - Funding Information: This research was supported by Gachon Bio-Nano Research Institute, Kyungwon University. We thank Prof. I.S. Yoo at the Kyungwon University and Dr. G.S. Lee at the Korea University for valuable discussions.

PY - 2008/3/15

Y1 - 2008/3/15

N2 - Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.

AB - Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.

KW - Adhesion

KW - Atomic force microscopy

KW - Deformation elasticity

KW - Jump-to-surface

KW - Langmuir-Blodgett technique

KW - Phase-separation

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U2 - 10.1016/j.colsurfb.2007.09.020

DO - 10.1016/j.colsurfb.2007.09.020

M3 - Article

C2 - 17959364

AN - SCOPUS:38849103087

SN - 0927-7765

VL - 62

SP - 157

EP - 161

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 1

ER -

Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

Abstract

Keywords

ASJC Scopus subject areas

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