Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

Jin Won Park, Dong June Ahn

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume62
Issue number1
DOIs
Publication statusPublished - 2008 Mar 15

Keywords

  • Adhesion
  • Atomic force microscopy
  • Deformation elasticity
  • Jump-to-surface
  • Langmuir-Blodgett technique
  • Phase-separation

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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