Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

Jin Won Park, Dong June Ahn

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume62
Issue number1
DOIs
Publication statusPublished - 2008 Mar 15

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1,2-Dipalmitoylphosphatidylcholine
Phospholipids
Thermal effects
temperature effects
Monolayers
Physical properties
physical properties
Temperature
Force measurement
Surface measurement
Mica
Phase separation
Elasticity
Microscopes
Adhesion
Surface Properties
mica
Imaging techniques
adhesion
elastic properties

Keywords

  • Adhesion
  • Atomic force microscopy
  • Deformation elasticity
  • Jump-to-surface
  • Langmuir-Blodgett technique
  • Phase-separation

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers. / Park, Jin Won; Ahn, Dong June.

In: Colloids and Surfaces B: Biointerfaces, Vol. 62, No. 1, 15.03.2008, p. 157-161.

Research output: Contribution to journalArticle

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