Temperature-specific vsiRNA confers RNAi-mediated viral resistance at elevated temperature in Capsicum annuum

Yunsik Kim, Young Jin Kim, Kyung Hee Paek

Research output: Contribution to journalArticlepeer-review

Abstract

Resistance (R) gene-mediated resistance is a robust and efficient antiviral immune system in the plants. Thus, when R-mediated resistance was suppressed at elevated temperatures, resistance towards viruses was expected to be completely collapsed. Nonetheless, the multiplication of Tobacco mosaic virus pathotype P0 (TMV-P0) was inhibited, and TMV-P0 particles were only occasionally present in the systemic leaves of pepper plants (Capsicum annuum). RNAi-mediated RNA silencing is a well-known antiviral immune mechanism. At elevated temperatures, RNAi-mediated antiviral resistance was induced and virus-derived siRNAs (vsiRNAs) were dramatically increased. Through sRNA-sequencing (sRNA-Seq) analysis, we revealed that vsiRNAs derived from TMV-P0 were greatly increased. Intriguingly, virus-infected plants could select the temperature-specific vsiRNAs for antiviral resistance from the amplified vsiRNAs at elevated temperatures. Pre-application of these temperature-specific vsiRNAs endowed antiviral resistance of the plants. Therefore, plants sustain antiviral resistance by activating RNAi-mediated resistance, based on temperature-specific vsiRNAs at elevated temperatures.

Original languageEnglish
Pages (from-to)1432-1448
Number of pages17
JournalJournal of experimental botany
Volume72
Issue number4
DOIs
Publication statusPublished - 2021 Feb 24

Keywords

  • Capsicum annuum (pepper)
  • R-mediated resistance
  • RNAi-mediated antiviral resistance
  • Tobacco mosaic virus pathotype P(TMV-P)
  • elevated temperatures
  • virus-derived siRNAs (vsiRNAs)

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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