TEMPO-Assisted Free Radical-Initiated Peptide Sequencing Mass Spectrometry (FRIPS MS) in Q-TOF and Orbitrap Mass Spectrometers: Single-Step Peptide Backbone Dissociations in Positive Ion Mode

Inae Jang, Sun Young Lee, Song Hwangbo, Dukjin Kang, Hookeun Lee, Hugh I. Kim, Bongjin Moon, Han Bin Oh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present study demonstrates that one-step peptide backbone fragmentations can be achieved using the TEMPO [2-(2,2,6,6-tetramethyl piperidine-1-oxyl)]-assisted free radical-initiated peptide sequencing (FRIPS) mass spectrometry in a hybrid quadrupole time-of-flight (Q-TOF) mass spectrometer and a Q-Exactive Orbitrap instrument in positive ion mode, in contrast to two-step peptide fragmentation in an ion-trap mass spectrometer (reference Anal. Chem. 85, 7044–7051 (30)). In the hybrid Q-TOF and Q-Exactive instruments, higher collisional energies can be applied to the target peptides, compared with the low collisional energies applied by the ion-trap instrument. The higher energy deposition and the additional multiple collisions in the collision cell in both instruments appear to result in one-step peptide backbone dissociations in positive ion mode. This new finding clearly demonstrates that the TEMPO-assisted FRIPS approach is a very useful tool in peptide mass spectrometry research. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)154-163
Number of pages10
JournalJournal of the American Society for Mass Spectrometry
Volume28
Issue number1
DOIs
Publication statusPublished - 2017 Jan 1

Keywords

  • Free radical-initiated peptide sequencing (FRIPS)
  • Orbitrap
  • Q-TOF
  • Radical-based fragmentations
  • TEMPO

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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