Abstract
The present study demonstrates that one-step peptide backbone fragmentations can be achieved using the TEMPO [2-(2,2,6,6-tetramethyl piperidine-1-oxyl)]-assisted free radical-initiated peptide sequencing (FRIPS) mass spectrometry in a hybrid quadrupole time-of-flight (Q-TOF) mass spectrometer and a Q-Exactive Orbitrap instrument in positive ion mode, in contrast to two-step peptide fragmentation in an ion-trap mass spectrometer (reference Anal. Chem. 85, 7044–7051 (30)). In the hybrid Q-TOF and Q-Exactive instruments, higher collisional energies can be applied to the target peptides, compared with the low collisional energies applied by the ion-trap instrument. The higher energy deposition and the additional multiple collisions in the collision cell in both instruments appear to result in one-step peptide backbone dissociations in positive ion mode. This new finding clearly demonstrates that the TEMPO-assisted FRIPS approach is a very useful tool in peptide mass spectrometry research. [Figure not available: see fulltext.]
Original language | English |
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Pages (from-to) | 154-163 |
Number of pages | 10 |
Journal | Journal of the American Society for Mass Spectrometry |
Volume | 28 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2017 Jan 1 |
Keywords
- Free radical-initiated peptide sequencing (FRIPS)
- Orbitrap
- Q-TOF
- Radical-based fragmentations
- TEMPO
ASJC Scopus subject areas
- Structural Biology
- Spectroscopy