Temporal changes in LHR and α and LHβ subunit messenger RNA levels in response to testosterone in castrated adult rats

M. Yoon, J. Y. Seong, E. Song, K. Ryu, K. Kim

Research output: Contribution to journalArticle

Abstract

To elucidate the temporal relationship between LHRH and LH gene expression in response to testosterone (T), LHRH mRNA in the hypothalamus, and α and LH β mRNA in the anterior pituitary were simultaneously determined. After Silastic capsules containing T were implanted subcutaneously to castrated (2 weeks) adult rats, LHRH, α and LH β mRNA levels were determined by RNA-blot hybridization assay and hypothalamic LHRH content and serum LH levels were measured by radioimmunoassays. T implant to castrated rats decreased α and LH β mRNA levels in time- and dose-dependent manners, which were accompanied by parallel change in serum LH levels. On the other hand, T implant did not significantly alter LHRH mRNA level, which was discordant with changes in α and LH β mRNA levels. The present study showed that T differentially regulates LHRH and LH mRNA levels, suggesting that the negative feedback action of T on LH gene expression may be mainly due to the direct action of T on pituitary gonadtropes.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalNeuroendocrinology Letters
Volume16
Issue number2
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Neurology
  • Endocrine and Autonomic Systems
  • Clinical Neurology
  • Psychiatry and Mental health

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