Abstract
Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.
| Original language | English |
|---|---|
| Journal | Journal of Dental Research |
| DOIs | |
| Publication status | Accepted/In press - 2018 Jan 1 |
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Keywords
- double tooth
- gain-of-function mutation
- loss-of-function mutation
- morphogenesis
- Wnt signaling pathway
- zebrafish
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Temporal Control of WNT Activity Regulates Tooth Number in Fish. / Shim, J. S.; Kim, B.; Park, Hae Chul; Ryu, Jae Jun.
In: Journal of Dental Research, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Temporal Control of WNT Activity Regulates Tooth Number in Fish
AU - Shim, J. S.
AU - Kim, B.
AU - Park, Hae Chul
AU - Ryu, Jae Jun
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.
AB - Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.
KW - double tooth
KW - gain-of-function mutation
KW - loss-of-function mutation
KW - morphogenesis
KW - Wnt signaling pathway
KW - zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85060532462&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060532462&partnerID=8YFLogxK
U2 - 10.1177/0022034518811322
DO - 10.1177/0022034518811322
M3 - Article
C2 - 30513239
AN - SCOPUS:85060532462
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
ER -