Temporal Control of WNT Activity Regulates Tooth Number in Fish

J. S. Shim, B. Kim, Hae Chul Park, Jae Jun Ryu

Research output: Contribution to journalArticle

Abstract

Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.

Original languageEnglish
JournalJournal of Dental Research
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Catenins
Tooth
Morphogenesis
Cell Polarity
Wnt Signaling Pathway
Zebrafish
Embryonic Development
Cell Differentiation
Hot Temperature
Cell Proliferation

Keywords

  • double tooth
  • gain-of-function mutation
  • loss-of-function mutation
  • morphogenesis
  • Wnt signaling pathway
  • zebrafish

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Temporal Control of WNT Activity Regulates Tooth Number in Fish. / Shim, J. S.; Kim, B.; Park, Hae Chul; Ryu, Jae Jun.

In: Journal of Dental Research, 01.01.2018.

Research output: Contribution to journalArticle

@article{47e5eed9c2e243e1831358e85155016e,
title = "Temporal Control of WNT Activity Regulates Tooth Number in Fish",
abstract = "Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.",
keywords = "double tooth, gain-of-function mutation, loss-of-function mutation, morphogenesis, Wnt signaling pathway, zebrafish",
author = "Shim, {J. S.} and B. Kim and Park, {Hae Chul} and Ryu, {Jae Jun}",
year = "2018",
month = "1",
day = "1",
doi = "10.1177/0022034518811322",
language = "English",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",

}

TY - JOUR

T1 - Temporal Control of WNT Activity Regulates Tooth Number in Fish

AU - Shim, J. S.

AU - Kim, B.

AU - Park, Hae Chul

AU - Ryu, Jae Jun

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.

AB - Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.

KW - double tooth

KW - gain-of-function mutation

KW - loss-of-function mutation

KW - morphogenesis

KW - Wnt signaling pathway

KW - zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85060532462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060532462&partnerID=8YFLogxK

U2 - 10.1177/0022034518811322

DO - 10.1177/0022034518811322

M3 - Article

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

ER -