Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain

Dongmin Lee, Meaghan Creed, Kanghoon Jung, Thomas Stefanelli, Daniel J. Wendler, Won Chan Oh, Neymi Layne Mignocchi, Christian Lüscher, Hyung Bae Kwon

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Few tools exist to visualize and manipulate neurons that are targets of neuromodulators. We present iTango, a light-and ligand-gated gene expression system based on a light-inducible split tobacco etch virus protease. Cells expressing the iTango system exhibit increased expression of a marker gene in the presence of dopamine and blue-light exposure, both in vitro and in vivo. We demonstrated the iTango system in a behaviorally relevant context, by inducing expression of optogenetic tools in neurons under dopaminergic control during a behavior of interest. We thereby gained optogenetic control of these behaviorally relevant neurons. We applied the iTango system to decipher the roles of two classes of dopaminergic neurons in the mouse nucleus accumbens in a sensitized locomotor response to cocaine. Thus, the iTango platform allows for control of neuromodulatory circuits in a genetically and functionally defined manner with spatial and temporal precision.

Original languageEnglish
Pages (from-to)495-503
Number of pages9
JournalNature Methods
Volume14
Issue number5
DOIs
Publication statusPublished - 2017 Apr 27

Fingerprint

Optogenetics
Labeling
Neurons
Brain
Dopaminergic Neurons
Light
Networks (circuits)
Nucleus Accumbens
Cocaine
Neurotransmitter Agents
Dopamine
Gene expression
Ligands
Gene Expression
Genes
Cells

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lee, D., Creed, M., Jung, K., Stefanelli, T., Wendler, D. J., Oh, W. C., ... Kwon, H. B. (2017). Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Nature Methods, 14(5), 495-503. https://doi.org/10.1038/nmeth.4234

Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. / Lee, Dongmin; Creed, Meaghan; Jung, Kanghoon; Stefanelli, Thomas; Wendler, Daniel J.; Oh, Won Chan; Mignocchi, Neymi Layne; Lüscher, Christian; Kwon, Hyung Bae.

In: Nature Methods, Vol. 14, No. 5, 27.04.2017, p. 495-503.

Research output: Contribution to journalArticle

Lee, D, Creed, M, Jung, K, Stefanelli, T, Wendler, DJ, Oh, WC, Mignocchi, NL, Lüscher, C & Kwon, HB 2017, 'Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain', Nature Methods, vol. 14, no. 5, pp. 495-503. https://doi.org/10.1038/nmeth.4234
Lee, Dongmin ; Creed, Meaghan ; Jung, Kanghoon ; Stefanelli, Thomas ; Wendler, Daniel J. ; Oh, Won Chan ; Mignocchi, Neymi Layne ; Lüscher, Christian ; Kwon, Hyung Bae. / Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. In: Nature Methods. 2017 ; Vol. 14, No. 5. pp. 495-503.
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