Tenacibaculum todarodis sp. Nov., isolated from a squid

Su Kyoung Shin, Eunji Kim, Hana Yi

Research output: Contribution to journalArticle

Abstract

A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0136T, was isolated from a squid Todarodes pacificus, caught in the East Sea, off Korea. LPB0136T contained a circular chromosome of 3.02 Mb with a DNA G+C content of 30.7 mol%. The genome included 2726 protein-coding genes and three copies of rRNA operons. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this isolate represents a member of the genus Tenacibaculum with an independent genomic species status, showing sequence similarities of 95.9% to Tenacibaculum aestuarii SMK-4T and Tenacibaculum caenipelagi HJ-26MT. The detected respiratory quinone (MK-6) and major polar lipid (phosphatidylethanolamine) were similar to the chemotaxonomic profile of other species of the genus Tenacibaculum. The major cellular fatty acids profile (iso-C15: 0, iso- C15: 0 3-OH and iso-C15: 0G) was also similar to those of members of genus Tenacibaculum, but the contents and amounts differed from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Tenacibaculum. On the basis of the pholyphasic taxonomic data determined in this study, strain LPB0136T represents a novel species of the genus Tenacibaculum, for which the name Tenacibaculum todarodis sp. nov. is proposed. The type strain is LPB0136T (=KACC 18887T=JCM 31564T).

Original languageEnglish
Article number002692
Pages (from-to)1479-1483
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number5
DOIs
Publication statusPublished - 2018 May 1

Fingerprint

Tenacibaculum
Decapodiformes
squid
gene
chromosome
genomics
fatty acid
genome
rRNA Operon
lipid
Todarodes pacificus
ribosomal RNA
phylogenetics
DNA
protein
Base Composition
operon
phosphatidylethanolamines
Korea
quinones

Keywords

  • Squid
  • Tenacibaculum
  • Tenacibaculum todarodis
  • Todarodes pacificus

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Tenacibaculum todarodis sp. Nov., isolated from a squid. / Shin, Su Kyoung; Kim, Eunji; Yi, Hana.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 5, 002692, 01.05.2018, p. 1479-1483.

Research output: Contribution to journalArticle

@article{24b61ea68e284cc080dce616836897c0,
title = "Tenacibaculum todarodis sp. Nov., isolated from a squid",
abstract = "A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0136T, was isolated from a squid Todarodes pacificus, caught in the East Sea, off Korea. LPB0136T contained a circular chromosome of 3.02 Mb with a DNA G+C content of 30.7 mol{\%}. The genome included 2726 protein-coding genes and three copies of rRNA operons. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this isolate represents a member of the genus Tenacibaculum with an independent genomic species status, showing sequence similarities of 95.9{\%} to Tenacibaculum aestuarii SMK-4T and Tenacibaculum caenipelagi HJ-26MT. The detected respiratory quinone (MK-6) and major polar lipid (phosphatidylethanolamine) were similar to the chemotaxonomic profile of other species of the genus Tenacibaculum. The major cellular fatty acids profile (iso-C15: 0, iso- C15: 0 3-OH and iso-C15: 0G) was also similar to those of members of genus Tenacibaculum, but the contents and amounts differed from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Tenacibaculum. On the basis of the pholyphasic taxonomic data determined in this study, strain LPB0136T represents a novel species of the genus Tenacibaculum, for which the name Tenacibaculum todarodis sp. nov. is proposed. The type strain is LPB0136T (=KACC 18887T=JCM 31564T).",
keywords = "Squid, Tenacibaculum, Tenacibaculum todarodis, Todarodes pacificus",
author = "Shin, {Su Kyoung} and Eunji Kim and Hana Yi",
year = "2018",
month = "5",
day = "1",
doi = "10.1099/ijsem.0.002692",
language = "English",
volume = "68",
pages = "1479--1483",
journal = "International Journal of Systematic Bacteriology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "5",

}

TY - JOUR

T1 - Tenacibaculum todarodis sp. Nov., isolated from a squid

AU - Shin, Su Kyoung

AU - Kim, Eunji

AU - Yi, Hana

PY - 2018/5/1

Y1 - 2018/5/1

N2 - A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0136T, was isolated from a squid Todarodes pacificus, caught in the East Sea, off Korea. LPB0136T contained a circular chromosome of 3.02 Mb with a DNA G+C content of 30.7 mol%. The genome included 2726 protein-coding genes and three copies of rRNA operons. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this isolate represents a member of the genus Tenacibaculum with an independent genomic species status, showing sequence similarities of 95.9% to Tenacibaculum aestuarii SMK-4T and Tenacibaculum caenipelagi HJ-26MT. The detected respiratory quinone (MK-6) and major polar lipid (phosphatidylethanolamine) were similar to the chemotaxonomic profile of other species of the genus Tenacibaculum. The major cellular fatty acids profile (iso-C15: 0, iso- C15: 0 3-OH and iso-C15: 0G) was also similar to those of members of genus Tenacibaculum, but the contents and amounts differed from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Tenacibaculum. On the basis of the pholyphasic taxonomic data determined in this study, strain LPB0136T represents a novel species of the genus Tenacibaculum, for which the name Tenacibaculum todarodis sp. nov. is proposed. The type strain is LPB0136T (=KACC 18887T=JCM 31564T).

AB - A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0136T, was isolated from a squid Todarodes pacificus, caught in the East Sea, off Korea. LPB0136T contained a circular chromosome of 3.02 Mb with a DNA G+C content of 30.7 mol%. The genome included 2726 protein-coding genes and three copies of rRNA operons. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this isolate represents a member of the genus Tenacibaculum with an independent genomic species status, showing sequence similarities of 95.9% to Tenacibaculum aestuarii SMK-4T and Tenacibaculum caenipelagi HJ-26MT. The detected respiratory quinone (MK-6) and major polar lipid (phosphatidylethanolamine) were similar to the chemotaxonomic profile of other species of the genus Tenacibaculum. The major cellular fatty acids profile (iso-C15: 0, iso- C15: 0 3-OH and iso-C15: 0G) was also similar to those of members of genus Tenacibaculum, but the contents and amounts differed from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Tenacibaculum. On the basis of the pholyphasic taxonomic data determined in this study, strain LPB0136T represents a novel species of the genus Tenacibaculum, for which the name Tenacibaculum todarodis sp. nov. is proposed. The type strain is LPB0136T (=KACC 18887T=JCM 31564T).

KW - Squid

KW - Tenacibaculum

KW - Tenacibaculum todarodis

KW - Todarodes pacificus

UR - http://www.scopus.com/inward/record.url?scp=85046553061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046553061&partnerID=8YFLogxK

U2 - 10.1099/ijsem.0.002692

DO - 10.1099/ijsem.0.002692

M3 - Article

C2 - 29521615

AN - SCOPUS:85046553061

VL - 68

SP - 1479

EP - 1483

JO - International Journal of Systematic Bacteriology

JF - International Journal of Systematic Bacteriology

SN - 1466-5026

IS - 5

M1 - 002692

ER -