TY - JOUR
T1 - Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay to characterize histopathologic changes following thermal injury
AU - Lee, Ji Min
AU - Park, Ji Hyun
AU - Kim, Bo Young
AU - Kim, Il Hwan
N1 - Funding Information:
This study was supported by Korea University Grant.
Publisher Copyright:
© The Korean Dermatological Association and The Korean Society for Investigative Dermatology.
PY - 2018/2
Y1 - 2018/2
N2 - Background: Despite the wide application of lasers and radiofrequency (RF) surgery in dermatology, it is difficult to find studies showing the extent of damage dependent on cell death. Objective: We evaluated histopathologic changes following in vivo thermal damage generated by CO2 laser, 1,444 nm long-pulsed neodymium:yttrium-Aluminum-garnet (LP Nd:YAG) laser and RF emitting electrosurgical unit. Methods: Thermal damage was induced by the above instruments on ventral skin of rat. Specimens were stained with hematoxylin and eosin, along with a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay, to highlight the degree of irreversible cellular injury. Results: The volume of vaporization was largest with the CO2 laser. Area of cell death area identified by TUNEL assay, when arranged from widest to narrowest, was 1,444 nm LP Nd:YAG laser, CO2 laser, and RF emitting electrosurgical unit. Conclusion: This histopathologic evaluation of the acute characterization of injury across devices may be advantageous for attaining better treatment outcomes.
AB - Background: Despite the wide application of lasers and radiofrequency (RF) surgery in dermatology, it is difficult to find studies showing the extent of damage dependent on cell death. Objective: We evaluated histopathologic changes following in vivo thermal damage generated by CO2 laser, 1,444 nm long-pulsed neodymium:yttrium-Aluminum-garnet (LP Nd:YAG) laser and RF emitting electrosurgical unit. Methods: Thermal damage was induced by the above instruments on ventral skin of rat. Specimens were stained with hematoxylin and eosin, along with a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay, to highlight the degree of irreversible cellular injury. Results: The volume of vaporization was largest with the CO2 laser. Area of cell death area identified by TUNEL assay, when arranged from widest to narrowest, was 1,444 nm LP Nd:YAG laser, CO2 laser, and RF emitting electrosurgical unit. Conclusion: This histopathologic evaluation of the acute characterization of injury across devices may be advantageous for attaining better treatment outcomes.
KW - Carbon dioxide lasers
KW - In situ nick-end labeling
KW - Neodymiumdoped yttrium aluminum garnet lasers
KW - Radiofrequency
KW - Thermal destruction
UR - http://www.scopus.com/inward/record.url?scp=85042537462&partnerID=8YFLogxK
U2 - 10.5021/ad.2018.30.1.41
DO - 10.5021/ad.2018.30.1.41
M3 - Article
AN - SCOPUS:85042537462
VL - 30
SP - 41
EP - 46
JO - Annals of Dermatology
JF - Annals of Dermatology
SN - 1013-9087
IS - 1
ER -