Abstract
Background: Despite the wide application of lasers and radiofrequency (RF) surgery in dermatology, it is difficult to find studies showing the extent of damage dependent on cell death. Objective: We evaluated histopathologic changes following in vivo thermal damage generated by CO2 laser, 1,444 nm long-pulsed neodymium:yttrium-Aluminum-garnet (LP Nd:YAG) laser and RF emitting electrosurgical unit. Methods: Thermal damage was induced by the above instruments on ventral skin of rat. Specimens were stained with hematoxylin and eosin, along with a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay, to highlight the degree of irreversible cellular injury. Results: The volume of vaporization was largest with the CO2 laser. Area of cell death area identified by TUNEL assay, when arranged from widest to narrowest, was 1,444 nm LP Nd:YAG laser, CO2 laser, and RF emitting electrosurgical unit. Conclusion: This histopathologic evaluation of the acute characterization of injury across devices may be advantageous for attaining better treatment outcomes.
| Original language | English |
|---|---|
| Pages (from-to) | 41-46 |
| Number of pages | 6 |
| Journal | Annals of Dermatology |
| Volume | 30 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2018 Feb 1 |
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Keywords
- Carbon dioxide lasers
- In situ nick-end labeling
- Neodymiumdoped yttrium aluminum garnet lasers
- Radiofrequency
- Thermal destruction
ASJC Scopus subject areas
- Dermatology
Cite this
Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay to characterize histopathologic changes following thermal injury. / Lee, Ji Min; Park, Ji Hyun; Kim, Bo Young; Kim, Il-Hwan.
In: Annals of Dermatology, Vol. 30, No. 1, 01.02.2018, p. 41-46.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay to characterize histopathologic changes following thermal injury
AU - Lee, Ji Min
AU - Park, Ji Hyun
AU - Kim, Bo Young
AU - Kim, Il-Hwan
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Background: Despite the wide application of lasers and radiofrequency (RF) surgery in dermatology, it is difficult to find studies showing the extent of damage dependent on cell death. Objective: We evaluated histopathologic changes following in vivo thermal damage generated by CO2 laser, 1,444 nm long-pulsed neodymium:yttrium-Aluminum-garnet (LP Nd:YAG) laser and RF emitting electrosurgical unit. Methods: Thermal damage was induced by the above instruments on ventral skin of rat. Specimens were stained with hematoxylin and eosin, along with a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay, to highlight the degree of irreversible cellular injury. Results: The volume of vaporization was largest with the CO2 laser. Area of cell death area identified by TUNEL assay, when arranged from widest to narrowest, was 1,444 nm LP Nd:YAG laser, CO2 laser, and RF emitting electrosurgical unit. Conclusion: This histopathologic evaluation of the acute characterization of injury across devices may be advantageous for attaining better treatment outcomes.
AB - Background: Despite the wide application of lasers and radiofrequency (RF) surgery in dermatology, it is difficult to find studies showing the extent of damage dependent on cell death. Objective: We evaluated histopathologic changes following in vivo thermal damage generated by CO2 laser, 1,444 nm long-pulsed neodymium:yttrium-Aluminum-garnet (LP Nd:YAG) laser and RF emitting electrosurgical unit. Methods: Thermal damage was induced by the above instruments on ventral skin of rat. Specimens were stained with hematoxylin and eosin, along with a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay, to highlight the degree of irreversible cellular injury. Results: The volume of vaporization was largest with the CO2 laser. Area of cell death area identified by TUNEL assay, when arranged from widest to narrowest, was 1,444 nm LP Nd:YAG laser, CO2 laser, and RF emitting electrosurgical unit. Conclusion: This histopathologic evaluation of the acute characterization of injury across devices may be advantageous for attaining better treatment outcomes.
KW - Carbon dioxide lasers
KW - In situ nick-end labeling
KW - Neodymiumdoped yttrium aluminum garnet lasers
KW - Radiofrequency
KW - Thermal destruction
UR - http://www.scopus.com/inward/record.url?scp=85042537462&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042537462&partnerID=8YFLogxK
U2 - 10.5021/ad.2018.30.1.41
DO - 10.5021/ad.2018.30.1.41
M3 - Article
VL - 30
SP - 41
EP - 46
JO - Annals of Dermatology
JF - Annals of Dermatology
SN - 1013-9087
IS - 1
ER -