Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay to characterize histopathologic changes following thermal injury

Ji Min Lee, Ji Hyun Park, Bo Young Kim, Il-Hwan Kim

Research output: Contribution to journalArticle

Abstract

Background: Despite the wide application of lasers and radiofrequency (RF) surgery in dermatology, it is difficult to find studies showing the extent of damage dependent on cell death. Objective: We evaluated histopathologic changes following in vivo thermal damage generated by CO2 laser, 1,444 nm long-pulsed neodymium:yttrium-Aluminum-garnet (LP Nd:YAG) laser and RF emitting electrosurgical unit. Methods: Thermal damage was induced by the above instruments on ventral skin of rat. Specimens were stained with hematoxylin and eosin, along with a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay, to highlight the degree of irreversible cellular injury. Results: The volume of vaporization was largest with the CO2 laser. Area of cell death area identified by TUNEL assay, when arranged from widest to narrowest, was 1,444 nm LP Nd:YAG laser, CO2 laser, and RF emitting electrosurgical unit. Conclusion: This histopathologic evaluation of the acute characterization of injury across devices may be advantageous for attaining better treatment outcomes.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalAnnals of Dermatology
Volume30
Issue number1
DOIs
Publication statusPublished - 2018 Feb 1

Fingerprint

Gas Lasers
DNA Nucleotidylexotransferase
Neodymium
Hot Temperature
Solid-State Lasers
Wounds and Injuries
Cell Death
Volatilization
Laser Therapy
Hematoxylin
Eosine Yellowish-(YS)
Transferases
Dermatology
Equipment and Supplies
Skin
deoxyuridine triphosphate

Keywords

  • Carbon dioxide lasers
  • In situ nick-end labeling
  • Neodymiumdoped yttrium aluminum garnet lasers
  • Radiofrequency
  • Thermal destruction

ASJC Scopus subject areas

  • Dermatology

Cite this

Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay to characterize histopathologic changes following thermal injury. / Lee, Ji Min; Park, Ji Hyun; Kim, Bo Young; Kim, Il-Hwan.

In: Annals of Dermatology, Vol. 30, No. 1, 01.02.2018, p. 41-46.

Research output: Contribution to journalArticle

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