TGF-β signaling preserves RECK expression in activated pancreatic stellate cells

Hong Sik Lee, Chae Seung Lim, Jungeun Lee, Nayoung Kim, Sangsu Bang, Hojae Lee, Bon Hong Min, Gil-Hong Park, Makoto Noda, William G. Stetler-Stevenson, Jun Seo Oh

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Activated pancreatic stellate cells (PSCs) play a pivotal role in the pathogenesis of pancreatic fibrosis, but the detailed mechanism for dysregulated accumulation of extracellular matrix (ECM) remains unclear. Cultured rat PSCs become activated by profibrogenic mediators, but these mediators failed to alter the expression levels of matrix metalloproteinases (MMPs) to the endogenous tissue inhibitors of metalloproteinases (TIMPs). Here, we examined the expression of RECK, a novel membrane-anchored MMP inhibitor, in PSCs. Although RECK mRNA levels were largely unchanged, RECK protein expression was barely detected at 2, 5 days after plating PSCs, but appeared following continued in vitro culture and cell passage which result in PSC activation. When PSCs at 5 days after plating (PSCs-5d) were treated with pepstatin A, an aspartic protease inhibitor, or TGF-β1, a profibrogenic mediator, RECK protein was detected in whole cell lysates. Conversely, Smad7 overexpression or suppression of Smad3 expression in PSCs after passage 2 (PSCs-P2) led to the loss of RECK protein expression. These findings suggest that RECK is post-translationally processed in preactivated PSCs but protected from proteolytic degradation by TGF-β signaling. Furthermore, collagenolytic activity of PSCs-5d was greatly reduced by TGF-β1, whereas that of PSCs-P2 was increased by anti-RECK antibody. Increased RECK levels were also observed in cerulein-induced acute pancreatitis. Therefore, our results suggest for the first time proteolytic processing of RECK as a mechanism regulating RECK activity, and demonstrate that TGF-β signaling in activated PSCs may promote ECM accumulation via a mechanism that preserves the protease inhibitory activity of RECK.

Original languageEnglish
Pages (from-to)1065-1074
Number of pages10
JournalJournal of Cellular Biochemistry
Volume104
Issue number3
DOIs
Publication statusPublished - 2008 Jun 1

Fingerprint

Pancreatic Stellate Cells
Plating
Ceruletide
Tissue Inhibitor of Metalloproteinases
Proteins
Matrix Metalloproteinase Inhibitors
Protease Inhibitors
Matrix Metalloproteinases
Rats
Peptide Hydrolases
Chemical activation
Membranes
Degradation
Messenger RNA
Antibodies
Processing
Extracellular Matrix

Keywords

  • Fibrosis
  • Pancreatic stellate cells
  • RECK
  • TGF-β

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

TGF-β signaling preserves RECK expression in activated pancreatic stellate cells. / Lee, Hong Sik; Lim, Chae Seung; Lee, Jungeun; Kim, Nayoung; Bang, Sangsu; Lee, Hojae; Min, Bon Hong; Park, Gil-Hong; Noda, Makoto; Stetler-Stevenson, William G.; Oh, Jun Seo.

In: Journal of Cellular Biochemistry, Vol. 104, No. 3, 01.06.2008, p. 1065-1074.

Research output: Contribution to journalArticle

Lee, Hong Sik ; Lim, Chae Seung ; Lee, Jungeun ; Kim, Nayoung ; Bang, Sangsu ; Lee, Hojae ; Min, Bon Hong ; Park, Gil-Hong ; Noda, Makoto ; Stetler-Stevenson, William G. ; Oh, Jun Seo. / TGF-β signaling preserves RECK expression in activated pancreatic stellate cells. In: Journal of Cellular Biochemistry. 2008 ; Vol. 104, No. 3. pp. 1065-1074.
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AU - Lee, Hong Sik

AU - Lim, Chae Seung

AU - Lee, Jungeun

AU - Kim, Nayoung

AU - Bang, Sangsu

AU - Lee, Hojae

AU - Min, Bon Hong

AU - Park, Gil-Hong

AU - Noda, Makoto

AU - Stetler-Stevenson, William G.

AU - Oh, Jun Seo

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AB - Activated pancreatic stellate cells (PSCs) play a pivotal role in the pathogenesis of pancreatic fibrosis, but the detailed mechanism for dysregulated accumulation of extracellular matrix (ECM) remains unclear. Cultured rat PSCs become activated by profibrogenic mediators, but these mediators failed to alter the expression levels of matrix metalloproteinases (MMPs) to the endogenous tissue inhibitors of metalloproteinases (TIMPs). Here, we examined the expression of RECK, a novel membrane-anchored MMP inhibitor, in PSCs. Although RECK mRNA levels were largely unchanged, RECK protein expression was barely detected at 2, 5 days after plating PSCs, but appeared following continued in vitro culture and cell passage which result in PSC activation. When PSCs at 5 days after plating (PSCs-5d) were treated with pepstatin A, an aspartic protease inhibitor, or TGF-β1, a profibrogenic mediator, RECK protein was detected in whole cell lysates. Conversely, Smad7 overexpression or suppression of Smad3 expression in PSCs after passage 2 (PSCs-P2) led to the loss of RECK protein expression. These findings suggest that RECK is post-translationally processed in preactivated PSCs but protected from proteolytic degradation by TGF-β signaling. Furthermore, collagenolytic activity of PSCs-5d was greatly reduced by TGF-β1, whereas that of PSCs-P2 was increased by anti-RECK antibody. Increased RECK levels were also observed in cerulein-induced acute pancreatitis. Therefore, our results suggest for the first time proteolytic processing of RECK as a mechanism regulating RECK activity, and demonstrate that TGF-β signaling in activated PSCs may promote ECM accumulation via a mechanism that preserves the protease inhibitory activity of RECK.

KW - Fibrosis

KW - Pancreatic stellate cells

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KW - TGF-β

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