The 35S promoter used in a selectable marker gene of a plant transformation vector affects the expression of the transgene

Yeon Yoo So, Kirsten Bomblies, Seungkwon You, Won Yang Jung, Suk Choi Mi, Seob Lee Jong, Detlef Weigel, Ji Hoon Ahn

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Positive selection of transgenic plants is essential during plant transformation. Thus, strong promoters are often used in selectable marker genes to ensure successful selection. Many plant transformation vectors, including pPZP family vectors, use the 35S promoter as a regulatory sequence for their selectable marker genes. We found that the 35S promoter used in a selectable marker gene affected the expression pattern of a transgene, possibly leading to a misinterpretation of the result obtained from transgenic plants. It is likely that the 35S enhancer sequence in the 35S promoter is responsible for the interference, as in the activation tagging screen. This affected expression mostly disappeared in transgenic plants generated using vectors without the 35S sequences within their T-DNA region. Therefore, we suggest that caution should be used in selecting a plant transformation vector and in the interpretation of the results obtained from transgenic approaches using vectors carrying the 35S promoter sequences within their T-DNA regions.

Original languageEnglish
Pages (from-to)523-530
Number of pages8
JournalPlanta
Volume221
Issue number4
DOIs
Publication statusPublished - 2005 Jun 1

Fingerprint

Plant Genes
Genetically Modified Plants
Transgenes
transgenes
promoter regions
genetic markers
transgenic plants
Genes
enhancer elements
regulatory sequences
Gene Expression
DNA
crossover interference
genetically modified organisms
gene expression
T-DNA

Keywords

  • 35S enhancer
  • 35S promoter
  • Misexpression
  • Plant transformation vector
  • Trans activation

ASJC Scopus subject areas

  • Plant Science

Cite this

The 35S promoter used in a selectable marker gene of a plant transformation vector affects the expression of the transgene. / So, Yeon Yoo; Bomblies, Kirsten; You, Seungkwon; Jung, Won Yang; Mi, Suk Choi; Jong, Seob Lee; Weigel, Detlef; Ahn, Ji Hoon.

In: Planta, Vol. 221, No. 4, 01.06.2005, p. 523-530.

Research output: Contribution to journalArticle

So, Yeon Yoo ; Bomblies, Kirsten ; You, Seungkwon ; Jung, Won Yang ; Mi, Suk Choi ; Jong, Seob Lee ; Weigel, Detlef ; Ahn, Ji Hoon. / The 35S promoter used in a selectable marker gene of a plant transformation vector affects the expression of the transgene. In: Planta. 2005 ; Vol. 221, No. 4. pp. 523-530.
@article{90122286e50f45319345db3cb15be536,
title = "The 35S promoter used in a selectable marker gene of a plant transformation vector affects the expression of the transgene",
abstract = "Positive selection of transgenic plants is essential during plant transformation. Thus, strong promoters are often used in selectable marker genes to ensure successful selection. Many plant transformation vectors, including pPZP family vectors, use the 35S promoter as a regulatory sequence for their selectable marker genes. We found that the 35S promoter used in a selectable marker gene affected the expression pattern of a transgene, possibly leading to a misinterpretation of the result obtained from transgenic plants. It is likely that the 35S enhancer sequence in the 35S promoter is responsible for the interference, as in the activation tagging screen. This affected expression mostly disappeared in transgenic plants generated using vectors without the 35S sequences within their T-DNA region. Therefore, we suggest that caution should be used in selecting a plant transformation vector and in the interpretation of the results obtained from transgenic approaches using vectors carrying the 35S promoter sequences within their T-DNA regions.",
keywords = "35S enhancer, 35S promoter, Misexpression, Plant transformation vector, Trans activation",
author = "So, {Yeon Yoo} and Kirsten Bomblies and Seungkwon You and Jung, {Won Yang} and Mi, {Suk Choi} and Jong, {Seob Lee} and Detlef Weigel and Ahn, {Ji Hoon}",
year = "2005",
month = "6",
day = "1",
doi = "10.1007/s00425-004-1466-4",
language = "English",
volume = "221",
pages = "523--530",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - The 35S promoter used in a selectable marker gene of a plant transformation vector affects the expression of the transgene

AU - So, Yeon Yoo

AU - Bomblies, Kirsten

AU - You, Seungkwon

AU - Jung, Won Yang

AU - Mi, Suk Choi

AU - Jong, Seob Lee

AU - Weigel, Detlef

AU - Ahn, Ji Hoon

PY - 2005/6/1

Y1 - 2005/6/1

N2 - Positive selection of transgenic plants is essential during plant transformation. Thus, strong promoters are often used in selectable marker genes to ensure successful selection. Many plant transformation vectors, including pPZP family vectors, use the 35S promoter as a regulatory sequence for their selectable marker genes. We found that the 35S promoter used in a selectable marker gene affected the expression pattern of a transgene, possibly leading to a misinterpretation of the result obtained from transgenic plants. It is likely that the 35S enhancer sequence in the 35S promoter is responsible for the interference, as in the activation tagging screen. This affected expression mostly disappeared in transgenic plants generated using vectors without the 35S sequences within their T-DNA region. Therefore, we suggest that caution should be used in selecting a plant transformation vector and in the interpretation of the results obtained from transgenic approaches using vectors carrying the 35S promoter sequences within their T-DNA regions.

AB - Positive selection of transgenic plants is essential during plant transformation. Thus, strong promoters are often used in selectable marker genes to ensure successful selection. Many plant transformation vectors, including pPZP family vectors, use the 35S promoter as a regulatory sequence for their selectable marker genes. We found that the 35S promoter used in a selectable marker gene affected the expression pattern of a transgene, possibly leading to a misinterpretation of the result obtained from transgenic plants. It is likely that the 35S enhancer sequence in the 35S promoter is responsible for the interference, as in the activation tagging screen. This affected expression mostly disappeared in transgenic plants generated using vectors without the 35S sequences within their T-DNA region. Therefore, we suggest that caution should be used in selecting a plant transformation vector and in the interpretation of the results obtained from transgenic approaches using vectors carrying the 35S promoter sequences within their T-DNA regions.

KW - 35S enhancer

KW - 35S promoter

KW - Misexpression

KW - Plant transformation vector

KW - Trans activation

UR - http://www.scopus.com/inward/record.url?scp=21344467469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21344467469&partnerID=8YFLogxK

U2 - 10.1007/s00425-004-1466-4

DO - 10.1007/s00425-004-1466-4

M3 - Article

VL - 221

SP - 523

EP - 530

JO - Planta

JF - Planta

SN - 0032-0935

IS - 4

ER -