The 5' untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell-free translation

Chang Seob Kwon, Kyung Hee Paek, Won I. Chung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3' untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5' UTR, not the 3' UTR, substantially contributed to the highly competitive activity of CMV RNA4.

Original languageEnglish
Pages (from-to)89-93
Number of pages5
JournalFEBS Letters
Volume422
Issue number1
DOIs
Publication statusPublished - 1998 Jan 23

Fingerprint

Cucumovirus
5' Untranslated Regions
Luciferases
Viruses
Virion
RNA
Messenger RNA
RNA Viruses
3' Untranslated Regions
Alfalfa mosaic virus
Untranslated Regions

Keywords

  • 5' Untranslated region
  • Competitive determinant
  • Cucumber mosaic virus
  • mRNA competition
  • Translation efficiency

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The 5' untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell-free translation. / Kwon, Chang Seob; Paek, Kyung Hee; Chung, Won I.

In: FEBS Letters, Vol. 422, No. 1, 23.01.1998, p. 89-93.

Research output: Contribution to journalArticle

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