The ABA effect on the accumulation of an invertase inhibitor transcript that is driven by the CAMV35S promoter in ARABIDOPSIS

Eun Ji Koh, Sung June Lee, Suk Whan Hong, Hoi Seon Lee, Hojoung Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Invertase (β-D-fructofuranosidase; EC 3.2.1.26) catalyzes the conversion of sucrose into glucose and fructose and is involved in an array of important processes, including phloem unloading, carbon partitioning, the response to pathogens, and the control of cell differentiation and development. Its importance may have caused the invertases to evolve into a multigene family whose members are regulated by a variety of different mechanisms, such as pH, sucrose levels, and inhibitor proteins. Although putative invertase inhibitors in the Arabidopsis genome are easy to locate, few studies have been conducted to elucidate their individual functions in vivo in plant growth and development because of their high redundancy. In this study we assessed the functional role of the putative invertase inhibitors in Arabidopsis by generating transgenic plants harboring a putative invertase inhibitor gene under the control of the CaMV35S promoter. A transgenic plant that expressed high levels of the putative invertase inhibitor transcript when grown under normal conditions was chosen for the current study. To our surprise, the stability of the invertase inhibitor transcripts was shown to be down-regulated by the phytohormone ABA (abscisic acid). It is well established that ABA enhances invertase activity in vivo but the underlying mechanisms are still poorly understood. Our results thus suggest that one way ABA regulates invertase activity is by down-regulating its inhibitor.

Original languageEnglish
Pages (from-to)236-242
Number of pages7
JournalMolecules and Cells
Volume26
Issue number3
Publication statusPublished - 2008 Sep 30

Fingerprint

beta-Fructofuranosidase
Abscisic Acid
Genetically Modified Plants
Arabidopsis
Sucrose
Phloem
Plant Growth Regulators
Plant Development
Multigene Family
Fructose
Growth and Development
Cell Differentiation
Carbon
Genome
Glucose

Keywords

  • ABA
  • Abiotic stress
  • Arabidopsis thaliana
  • Invertase
  • Invertase inhibitor
  • Salt stress

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The ABA effect on the accumulation of an invertase inhibitor transcript that is driven by the CAMV35S promoter in ARABIDOPSIS. / Koh, Eun Ji; Lee, Sung June; Hong, Suk Whan; Lee, Hoi Seon; Lee, Hojoung.

In: Molecules and Cells, Vol. 26, No. 3, 30.09.2008, p. 236-242.

Research output: Contribution to journalArticle

Koh, Eun Ji ; Lee, Sung June ; Hong, Suk Whan ; Lee, Hoi Seon ; Lee, Hojoung. / The ABA effect on the accumulation of an invertase inhibitor transcript that is driven by the CAMV35S promoter in ARABIDOPSIS. In: Molecules and Cells. 2008 ; Vol. 26, No. 3. pp. 236-242.
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