Abstract
We tried to semiquantitize HBV DNA and reevaluate how the amount of HBV DNA affects the aminotransferases in blood using 60 chronic hepatitis B patients who were divided into 6 groups depending on HBsAg, HbeAg/Ab and aminotransferases. Our results showed that the amount of HBV DNA has no correlationship with aminotransferases in chronic hepatitis B patients whose HBsAg and HBeAg are both positive. However, the amount of HBV DNA is generally in low level in HbsAg (+), HBeAb (+) patients whose aminotransferases are within a normal range. In contrast, the amount of HBV DNA and aminotransferases has no correlationship in HBsAg (+), HBeAb (+) patients when aminotransferases are high. To find multiple HBV mutants in a single patient and diverse mutant populations in chronic hepatitis B patients, we analyzed the amplifed PCR products of HBV core region using single strand conformational polymorphysm (SSCP) and DNA sequecing. Our results showed that multiple HBV mutants coexist in a single patient and that t[BV mutant populations are different from patients to patients. These observation suggest that enormous populations of ttBV mutants exist in chronic hepatitis B patients.
Original language | English |
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Pages (from-to) | A1437 |
Journal | FASEB Journal |
Volume | 11 |
Issue number | 9 |
Publication status | Published - 1997 |
Externally published | Yes |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Genetics