The analysis of differentially expressed novel transcripts in diapausing and diapause-activated eggs of Bombyx mori

Jae Sam Hwang, Hyun Jeong Go, Tae Won Goo, Eun Young Yun, Kwang Ho Choi, Su Il Seong, Sang Mong Lee, Bong Hee Lee, Iksoo Kim, Taehoon Chun, Seok Woo Kang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

As an initial step to define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworms, Bombyx mori, mRNA transcripts from maintained and activated diapause eggs were compared with differential expression using cDNA array. Twenty-four individual cDNA transcripts were expressed differentially in a total of 1,468 different cDNAs. Among those clones, mRNA transcript from cytochrome oxidase subunit I (COI), which was detected to be 2-kb transcripts, gradually increased in diapause-activated eggs during early embryogenesis. Further analysis revealed that mRNA transcripts from silkworm COI were highly expressed in testis, fat body, and midgut during the larval stage. These results may indicate that the expression of silkworm COI mRNA is regulated developmentally as well as tissue-specifically.

Original languageEnglish
Pages (from-to)197-201
Number of pages5
JournalArchives of Insect Biochemistry and Physiology
Volume59
Issue number4
DOIs
Publication statusPublished - 2005 Aug
Externally publishedYes

Keywords

  • Cytochrome oxidase subunit I
  • DNA array
  • Diapause
  • Embryogenesis
  • Insects
  • Silkworm

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

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    Hwang, J. S., Go, H. J., Goo, T. W., Yun, E. Y., Choi, K. H., Seong, S. I., Lee, S. M., Lee, B. H., Kim, I., Chun, T., & Kang, S. W. (2005). The analysis of differentially expressed novel transcripts in diapausing and diapause-activated eggs of Bombyx mori. Archives of Insect Biochemistry and Physiology, 59(4), 197-201. https://doi.org/10.1002/arch.20057