The antioxidant property of chitosan green tea polyphenols complex induces transglutaminase activation in wound healing

Yao Qin, Xing Wei Guo, Lei Li, Hong Wei Wang, Wook Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The present study examined, for the first time, the in vitro wound healing potential of chitosan green tea polyphenols (CGP) complex based on the activation of transglutaminase (TGM) genes in epidermal morphogenesis. Response surface methodology was applied to determine the optimal processing condition that gave maximum extraction of green tea polyphenols. The antioxidant activity, scavenging ability, and chelating ability were studied and expressed as average EC50 values of CGP and other treatments. In silico analysis and gene coexpression network was subjected to the TGM sequences analysis. The temporal expressions of TGMs were profiled by semi-quantitative reverse transcription (RT)-PCR technology within 10 days after wounding and 2 days postwounding. CGP showed the effectiveness of antioxidant properties, and the observations of histopathological photography showed advanced tissue granulation and epithelialization formation by CGP treatment. In silico and coexpression analysis confirmed the regulation via TGM gene family in dermatological tissues. RT-PCR demonstrated increased levels of TGM1-3 expression induced by CGP treatment. The efficacy of CGP in wound healing based on these results may be ascribed to its antioxidant properties and activation of the expression of TGMs, and is, thus, essential for the facilitated repair of skin injury.

Original languageEnglish
Pages (from-to)487-498
Number of pages12
JournalJournal of Medicinal Food
Volume16
Issue number6
DOIs
Publication statusPublished - 2013 Jun 1

Fingerprint

Transglutaminases
Chitosan
Polyphenols
Tea
Wound Healing
Antioxidants
Computer Simulation
Reverse Transcription
Polymerase Chain Reaction
Granulation Tissue
Gene Regulatory Networks
Photography
Morphogenesis
Transcriptional Activation
Sequence Analysis
Technology
Skin
Wounds and Injuries
Genes

Keywords

  • Chitosan
  • Epidermis
  • Polyphenols
  • Wound healing

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

The antioxidant property of chitosan green tea polyphenols complex induces transglutaminase activation in wound healing. / Qin, Yao; Guo, Xing Wei; Li, Lei; Wang, Hong Wei; Kim, Wook.

In: Journal of Medicinal Food, Vol. 16, No. 6, 01.06.2013, p. 487-498.

Research output: Contribution to journalArticle

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