The arabidopsis calcium sensor calcineurin B-like 3 inhibits the 5′-methylthioadenosine nucleosidase in a calcium-dependent manner

Seung Ick Oh, Jimyeong Park, Sunhee Yoon, Yungyeong Kim, Soojin Park, Migyeong Ryu, Jung Nam Min, Han Ok Sung, Jeong Kook Kim, Jeong Sheop Shin, Kyung Nam Kim

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Calcineurin B-like (CBL) proteins represent a unique family of calcium sensors in plant cells. Sensing the calcium signals elicited by a variety of abiotic stresses, CBLs transmit the information to a group of serine/threonine protein kinases (CBLinteracting protein kinases [CIPKs]), which are currently known as the sole targets of the CBL family. Here, we report that the CBL3 member of this family has a novel interaction partner in addition to the CIPK proteins. Extensive yeast two-hybrid screenings with CBL3 as bait identified an interesting Arabidopsis (Arabidopsis thaliana) cDNA clone (named AtMTAN, for 5′-methylthioadenosine nucleosidase), which encodes a polypeptide similar to EcMTAN from Escherichia coli. Deletion analyses showed that CBL3 utilizes the different structural modules to interact with its distinct target proteins, CIPKs and AtMTAN. In vitro and in vivo analyses verified that CBL3 and AtMTAN physically associate only in the presence of Ca2+. In addition, we empirically demonstrated that the AtMTAN protein indeed possesses the MTAN activity, which can be inhibited specifically by Ca2+-bound CBL3. Overall, these findings suggest that the CBL family members can relay the calcium signals in more diverse ways than previously thought. We also discuss a possible mechanism by which the CBL3-mediated calcium signaling regulates the biosynthesis of ethylene and polyamines, which are involved in plant growth and development as well as various stress responses.

Original languageEnglish
Pages (from-to)1883-1896
Number of pages14
JournalPlant Physiology
Volume148
Issue number4
DOIs
Publication statusPublished - 2008 Dec 1

Fingerprint

Calcineurin
Arabidopsis
Protein Kinases
Calcium
calcium
protein kinases
Proteins
proteins
Calcium Signaling
Plant Development
Protein-Serine-Threonine Kinases
Polyamines
Plant Cells
two hybrid system techniques
Growth and Development
polyamines
Complementary DNA
Clone Cells
Yeasts
ethylene production

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

The arabidopsis calcium sensor calcineurin B-like 3 inhibits the 5′-methylthioadenosine nucleosidase in a calcium-dependent manner. / Oh, Seung Ick; Park, Jimyeong; Yoon, Sunhee; Kim, Yungyeong; Park, Soojin; Ryu, Migyeong; Min, Jung Nam; Sung, Han Ok; Kim, Jeong Kook; Shin, Jeong Sheop; Kim, Kyung Nam.

In: Plant Physiology, Vol. 148, No. 4, 01.12.2008, p. 1883-1896.

Research output: Contribution to journalArticle

Oh, Seung Ick ; Park, Jimyeong ; Yoon, Sunhee ; Kim, Yungyeong ; Park, Soojin ; Ryu, Migyeong ; Min, Jung Nam ; Sung, Han Ok ; Kim, Jeong Kook ; Shin, Jeong Sheop ; Kim, Kyung Nam. / The arabidopsis calcium sensor calcineurin B-like 3 inhibits the 5′-methylthioadenosine nucleosidase in a calcium-dependent manner. In: Plant Physiology. 2008 ; Vol. 148, No. 4. pp. 1883-1896.
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