The arabidopsis cysteine-rich receptor-like kinase crk36 regulates immunity through interaction with the cytoplasmic kinase BIK1

Dong Sook Lee, Young Cheon Kim, Sun Jae Kwon, Choong Min Ryu, Ohkmae Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Receptor-like kinases are important signaling components that regulate a variety of cellular processes. In this study, an Arabidopsis cDNA microarray analysis led to the identification of the cysteine-rich receptor-like kinase CRK36 responsive to the necrotrophic fungal pathogen, Alternaria brassicicola. To determine the function of CRK36 in plant immunity, T-DNA-insertion knockdown (crk36) and overexpressing (CRK36OE) plants were prepared. CRK36OE plants exhibited increased hypersensitive cell death and ROS burst in response to avirulent pathogens. Treatment with a typical pathogen-associated molecular pattern, flg22, markedly induced pattern-triggered immune responses, notably stomatal defense, in CRK36OE plants. The immune responses were weakened in crk36 plants. Protein-protein interaction assays revealed the in vivo association of CRK36, FLS2, and BIK1. CRK36 enhanced flg22-triggered BIK1 phosphorylation, which showed defects with Cys mutations in the DUF26 motifs of CRK36. Disruption of BIK1 and RbohD/RbohF genes further impaired CRK36-mediated stomatal defense. We propose that CRK36, together with BIK1 and NADPH oxidases, may form a positive activation loop that enhances ROS burst and leads to the promotion of stomatal immunity.

Original languageEnglish
Article number1856
JournalFrontiers in Plant Science
Volume8
DOIs
Publication statusPublished - 2017 Oct 27

Fingerprint

cysteine
phosphotransferases (kinases)
immunity
Arabidopsis
receptors
pathogens
immune response
Alternaria brassicicola
protein-protein interactions
cell death
phosphorylation
mutation
DNA
assays
genes

Keywords

  • Arabidopsis thaliana
  • BiK1
  • CRK36
  • Cysteine-rich receptor-like kinase
  • FLS2
  • NADPH oxidase
  • Pattern-triggered immunity
  • Stomatal immunity

ASJC Scopus subject areas

  • Plant Science

Cite this

The arabidopsis cysteine-rich receptor-like kinase crk36 regulates immunity through interaction with the cytoplasmic kinase BIK1. / Lee, Dong Sook; Kim, Young Cheon; Kwon, Sun Jae; Ryu, Choong Min; Kim, Ohkmae.

In: Frontiers in Plant Science, Vol. 8, 1856, 27.10.2017.

Research output: Contribution to journalArticle

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