The Arabidopsis NAC transcription factor NTL4 participates in a positive feedback loop that induces programmed cell death under heat stress conditions

Sangmin Lee, Hyo Jun Lee, Sung Un Huh, Kyung Hee Paek, Jun Ho Ha, Chung Mo Park

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Programmed cell death (PCD) is an integral component of plant development and adaptation under adverse environmental conditions. Reactive oxygen species (ROS) are one of the most important players that trigger PCD in plants, and ROS-generating machinery is activated in plant cells undergoing PCD. The membrane-bound NAC transcription factor NTL4 has recently been proven to facilitate ROS production in response to drought stress in Arabidopsis. In this work, we show that NTL4 participates in a positive feedback loop that bursts ROS accumulation to modulate PCD under heat stress conditions. Heat stress induces NTL4 gene transcription and NTL4 protein processing. The level of hydrogen peroxide (H2O2) was elevated in 35S:4δC transgenic plants that overexpress a transcriptionally active nuclear NTL4 form but significantly reduced in NTL4-deficient ntl4 mutants under heat stress conditions. In addition, heat stress-induced cell death was accelerated in the 35S:4δC transgenic plants but decreased in the ntl4 mutants. Notably, H2O2 triggers NTL4 gene transcription and NTL4 protein processing under heat stress conditions. On the basis of these findings, we conclude that NTL4 modulates PCD through a ROS-mediated positive feedback control under heat stress conditions, possibly providing an adaptation strategy by which plants ensure their survival under extreme heat stress conditions.

Original languageEnglish
Pages (from-to)76-83
Number of pages8
JournalPlant Science
Volume227
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Arabidopsis
heat stress
Cell Death
Transcription Factors
apoptosis
transcription factors
Hot Temperature
Reactive Oxygen Species
reactive oxygen species
Genetically Modified Plants
Extreme Heat
transgenic plants
transcription (genetics)
Plant Development
Droughts
Plant Cells
mutants
plant adaptation
Hydrogen Peroxide
Genes

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Agronomy and Crop Science

Cite this

The Arabidopsis NAC transcription factor NTL4 participates in a positive feedback loop that induces programmed cell death under heat stress conditions. / Lee, Sangmin; Lee, Hyo Jun; Huh, Sung Un; Paek, Kyung Hee; Ha, Jun Ho; Park, Chung Mo.

In: Plant Science, Vol. 227, 01.01.2014, p. 76-83.

Research output: Contribution to journalArticle

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