The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms

Hyo Young Park, Keh Chien Lee, Yun Hee Jang, Soon Kap Kim, May Phyo Thu, Jeong Hwan Lee, Jeong Kook Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Key message: TheArabidopsissplicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. These proteins also move rapidly and continuously in the nuclei, and their movements are affected by ATP depletion. Abstract: The U2AF65 proteins are splicing factors that interact with SF1 and U2AF35 proteins to promote U2snRNP for the recognition of the pre-mRNA 3′ splice site during early spliceosome assembly. We have determined the subcellular localization and movement of these proteins’ Arabidopsis homologs. It was found that Arabidopsis U2AF65 homologs, AtU2AF65a, and AtU2AF65b proteins interact with AtU2AF35a and AtU2AF35b, which are Arabidopsis U2AF35 homologs. We have examined the mobility of these proteins including AtSF1 using fluorescence recovery after photobleaching and fluorescence loss in photobleaching analyses. These proteins displayed dynamic movements in nuclei and their movements were affected by ATP depletion. We have also demonstrated that these proteins shuttle between nuclei and cytoplasms, suggesting that they may also function in cytoplasm. These results indicate that such splicing factors show very similar characteristics to their human counterparts, suggesting evolutionary conservation.

Original languageEnglish
Pages (from-to)1113-1123
Number of pages11
JournalPlant Cell Reports
Volume36
Issue number7
DOIs
Publication statusPublished - 2017 Jul 1

Fingerprint

cytoplasm
Arabidopsis
proteins
spliceosomes
photobleaching
fluorescence

Keywords

  • Dynamic movement
  • FLIP
  • FRAP
  • SF1
  • Shuttling
  • U2AF35
  • U2AF65

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. / Park, Hyo Young; Lee, Keh Chien; Jang, Yun Hee; Kim, Soon Kap; Thu, May Phyo; Lee, Jeong Hwan; Kim, Jeong Kook.

In: Plant Cell Reports, Vol. 36, No. 7, 01.07.2017, p. 1113-1123.

Research output: Contribution to journalArticle

Park, Hyo Young ; Lee, Keh Chien ; Jang, Yun Hee ; Kim, Soon Kap ; Thu, May Phyo ; Lee, Jeong Hwan ; Kim, Jeong Kook. / The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. In: Plant Cell Reports. 2017 ; Vol. 36, No. 7. pp. 1113-1123.
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