The Aspergillus fumigatus StuA protein governs the up-regulation of a discrete transcriptional program during the acquisition of developmental competence

Donald C. Sheppard, Thomas Doedt, Lisa Y. Chiang, Heenam Kim, Dan Chen, William C. Nierman, Scott G. Filler

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Members of the Asm1p, Phd1p, Sok2p, Efg1p, and StuAp (APSES) family of fungal proteins regulate morphogenesis and virulence in ascomycetes. We cloned the Aspergillus fumigatus APSES gene encoding StuAp and demonstrated that stuA transcription is markedly up-regulated after the acquisition of developmental competence. A. fumigatus ΔstuA mutants were impaired in their ability to undergo asexual reproduction. Conidiophore morphology was markedly abnormal, and only small numbers of dysmorphic conidia were produced, which exhibited precocious germination. Whole genome transcriptional analysis during the onset of developmental competence was performed and identified a subset of developmentally regulated genes that were stuA dependent, including a cluster of putative secondary metabolite biosynthesis genes, genes encoding proteins implicated in the regulation of morphogenesis, and genes encoding allergens and other antigenic proteins. Additionally, hyphae of the ΔstuA mutant displayed reduced expression of the catalase gene CAT1 and were hypersusceptible to hydrogen peroxide.

Original languageEnglish
Pages (from-to)5866-5879
Number of pages14
JournalMolecular Biology of the Cell
Volume16
Issue number12
DOIs
Publication statusPublished - 2005 Dec 1
Externally publishedYes

Fingerprint

Aspergillus fumigatus
Mental Competency
Up-Regulation
Morphogenesis
Genes
Proteins
Asexual Reproduction
Ascomycota
Aptitude
Fungal Proteins
Fungal Spores
Hyphae
Germination
Catalase
Allergens
Hydrogen Peroxide
Virulence
Genome
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The Aspergillus fumigatus StuA protein governs the up-regulation of a discrete transcriptional program during the acquisition of developmental competence. / Sheppard, Donald C.; Doedt, Thomas; Chiang, Lisa Y.; Kim, Heenam; Chen, Dan; Nierman, William C.; Filler, Scott G.

In: Molecular Biology of the Cell, Vol. 16, No. 12, 01.12.2005, p. 5866-5879.

Research output: Contribution to journalArticle

Sheppard, Donald C. ; Doedt, Thomas ; Chiang, Lisa Y. ; Kim, Heenam ; Chen, Dan ; Nierman, William C. ; Filler, Scott G. / The Aspergillus fumigatus StuA protein governs the up-regulation of a discrete transcriptional program during the acquisition of developmental competence. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 12. pp. 5866-5879.
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