The bone regenerative effect of chitosan microsphere-encapsulated growth hormone on bony consolidation in mandibular distraction osteogenesis in a dog model.

Byung Chae Cho, Jong Yeop Kim, Jong Hyup Lee, Ho Yun Chung, Jae Woo Park, Kyung Ho Roh, G. U. Kim, Ick Chan Kwon, Kwang Ho Jang, Dong Sin Lee, Nang Woon Park, In-San Kim

Research output: Contribution to journalArticle

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Abstract

The purpose of this project was to study the effect of chitosan microsphere-encapsulated human growth hormone, which causes sustained release of chitosan and human growth hormone after implantation on early bony consolidation in distraction osteogenesis of a canine model. Forty-eight dogs were used for this study. An external distraction device was applied to the mandibular body after a vertical osteotomy, and the mandibular distraction was started 5 days after the operation at a rate of 1 mm/d up to a 10-mm distraction. The experimental group was divided into a control group (I), hyaluronic acid group (II), chitosan microsphere group (III), and chitosan microsphere-encapsulated human growth hormone group (IV). Normal saline was injected in group I. In group II, a 1-ml volume of hyaluronic acid solution was injected into the distracted area. In the group III, powder of chitosan microspheres and hGH were mixed with a 1-ml volume of hyaluronic acid to make an injectable form, and it was implanted into the distracted area. In group IV, powder of chitosan microsphere-encapsulated hGH was mixed with a 1-ml volume of hyaluronic acid. A total of 1-ml volume of the solution mix was implanted into the distracted area. Five dogs in each group (total of 20 dogs) were killed 3 weeks after completion of distraction. Twenty-eight dogs were killed at 6 weeks. Bone mineral density was 13.1% of the contralateral normal mandible at 3 weeks and 29.6% at 6 weeks in group I, 16.4% at 3 weeks and 40.4% at 6 weeks in group II, 16.6% at 3 weeks and 45.95% at 6 weeks in group III, and 29.6% at 3 weeks and 66.7% at 6 weeks in group IV. The mean three-point failure load was 16.1% in the control group, 34.7% in group II, 41.5% in group III, and 52.1% in group IV compared with the intact contralateral mandible, with statistical significance. In the histological findings, new bone was generated in all groups. In group IV, the formation of active woven bone was observed throughout the distracted area at 6 weeks. The amount of new bone formation in the distracted zone was in the order of group IV, group III, group II, and the control group. In conclusion, these findings suggest that chitosan microsphere-encapsulated hGH seems to be quite effective in early bone consolidation in distraction osteogenesis.

Original languageEnglish
Pages (from-to)299-311; discussion 312
JournalThe Journal of craniofacial surgery
Volume15
Issue number2
Publication statusPublished - 2004 Mar 1
Externally publishedYes

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Distraction Osteogenesis
Chitosan
Microspheres
Growth Hormone
Dogs
Bone and Bones
Hyaluronic Acid
Human Growth Hormone
Mandible
Powders
Control Groups
Mandibular Osteotomy
Osteogenesis
Bone Density
Canidae
Equipment and Supplies
Injections

ASJC Scopus subject areas

  • Surgery

Cite this

The bone regenerative effect of chitosan microsphere-encapsulated growth hormone on bony consolidation in mandibular distraction osteogenesis in a dog model. / Cho, Byung Chae; Kim, Jong Yeop; Lee, Jong Hyup; Chung, Ho Yun; Park, Jae Woo; Roh, Kyung Ho; Kim, G. U.; Kwon, Ick Chan; Jang, Kwang Ho; Lee, Dong Sin; Park, Nang Woon; Kim, In-San.

In: The Journal of craniofacial surgery, Vol. 15, No. 2, 01.03.2004, p. 299-311; discussion 312.

Research output: Contribution to journalArticle

Cho, BC, Kim, JY, Lee, JH, Chung, HY, Park, JW, Roh, KH, Kim, GU, Kwon, IC, Jang, KH, Lee, DS, Park, NW & Kim, I-S 2004, 'The bone regenerative effect of chitosan microsphere-encapsulated growth hormone on bony consolidation in mandibular distraction osteogenesis in a dog model.', The Journal of craniofacial surgery, vol. 15, no. 2, pp. 299-311; discussion 312.
Cho, Byung Chae ; Kim, Jong Yeop ; Lee, Jong Hyup ; Chung, Ho Yun ; Park, Jae Woo ; Roh, Kyung Ho ; Kim, G. U. ; Kwon, Ick Chan ; Jang, Kwang Ho ; Lee, Dong Sin ; Park, Nang Woon ; Kim, In-San. / The bone regenerative effect of chitosan microsphere-encapsulated growth hormone on bony consolidation in mandibular distraction osteogenesis in a dog model. In: The Journal of craniofacial surgery. 2004 ; Vol. 15, No. 2. pp. 299-311; discussion 312.
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N2 - The purpose of this project was to study the effect of chitosan microsphere-encapsulated human growth hormone, which causes sustained release of chitosan and human growth hormone after implantation on early bony consolidation in distraction osteogenesis of a canine model. Forty-eight dogs were used for this study. An external distraction device was applied to the mandibular body after a vertical osteotomy, and the mandibular distraction was started 5 days after the operation at a rate of 1 mm/d up to a 10-mm distraction. The experimental group was divided into a control group (I), hyaluronic acid group (II), chitosan microsphere group (III), and chitosan microsphere-encapsulated human growth hormone group (IV). Normal saline was injected in group I. In group II, a 1-ml volume of hyaluronic acid solution was injected into the distracted area. In the group III, powder of chitosan microspheres and hGH were mixed with a 1-ml volume of hyaluronic acid to make an injectable form, and it was implanted into the distracted area. In group IV, powder of chitosan microsphere-encapsulated hGH was mixed with a 1-ml volume of hyaluronic acid. A total of 1-ml volume of the solution mix was implanted into the distracted area. Five dogs in each group (total of 20 dogs) were killed 3 weeks after completion of distraction. Twenty-eight dogs were killed at 6 weeks. Bone mineral density was 13.1% of the contralateral normal mandible at 3 weeks and 29.6% at 6 weeks in group I, 16.4% at 3 weeks and 40.4% at 6 weeks in group II, 16.6% at 3 weeks and 45.95% at 6 weeks in group III, and 29.6% at 3 weeks and 66.7% at 6 weeks in group IV. The mean three-point failure load was 16.1% in the control group, 34.7% in group II, 41.5% in group III, and 52.1% in group IV compared with the intact contralateral mandible, with statistical significance. In the histological findings, new bone was generated in all groups. In group IV, the formation of active woven bone was observed throughout the distracted area at 6 weeks. The amount of new bone formation in the distracted zone was in the order of group IV, group III, group II, and the control group. In conclusion, these findings suggest that chitosan microsphere-encapsulated hGH seems to be quite effective in early bone consolidation in distraction osteogenesis.

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