The calcium-activated chloride channel anoctamin 1 acts as a heat sensor in nociceptive neurons

Hawon Cho, Young Duk Yang, Jesun Lee, Byeongjoon Lee, Tahnbee Kim, Yongwoo Jang, Seung Keun Back, Heung Sik Na, Brian D. Harfe, Fan Wang, Ramin Raouf, John N. Wood, Uhtaek Oh

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

Nociceptors are a subset of small primary afferent neurons that respond to noxious chemical, thermal and mechanical stimuli. Ion channels in nociceptors respond differently to noxious stimuli and generate electrical signals in different ways. Anoctamin 1 (ANO1 also known as TMEM16A) is a Ca 2+-activated chloride channel that is essential for numerous physiological functions. We found that ANO1 was activated by temperatures over 44 °C with steep heat sensitivity. ANO1 was expressed in small sensory neurons and was highly colocalized with nociceptor markers, which suggests that it may be involved in nociception. Application of heat ramps to dorsal root ganglion (DRG) neurons elicited robust ANO1-dependent depolarization. Furthermore, knockdown or deletion of ANO1 in DRG neurons substantially reduced nociceptive behavior in thermal pain models. These results indicate that ANO1 is a heat sensor that detects nociceptive thermal stimuli in sensory neurons and possibly mediates nociception.

Original languageEnglish
Pages (from-to)1015-1021
Number of pages7
JournalNature Neuroscience
Volume15
Issue number7
DOIs
Publication statusPublished - 2012 Jul 1

Fingerprint

Nociceptors
Chloride Channels
Hot Temperature
Nociception
Spinal Ganglia
Sensory Receptor Cells
Neurons
Afferent Neurons
Architectural Accessibility
Ion Channels
Pain
Temperature

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cho, H., Yang, Y. D., Lee, J., Lee, B., Kim, T., Jang, Y., ... Oh, U. (2012). The calcium-activated chloride channel anoctamin 1 acts as a heat sensor in nociceptive neurons. Nature Neuroscience, 15(7), 1015-1021. https://doi.org/10.1038/nn.3111

The calcium-activated chloride channel anoctamin 1 acts as a heat sensor in nociceptive neurons. / Cho, Hawon; Yang, Young Duk; Lee, Jesun; Lee, Byeongjoon; Kim, Tahnbee; Jang, Yongwoo; Back, Seung Keun; Na, Heung Sik; Harfe, Brian D.; Wang, Fan; Raouf, Ramin; Wood, John N.; Oh, Uhtaek.

In: Nature Neuroscience, Vol. 15, No. 7, 01.07.2012, p. 1015-1021.

Research output: Contribution to journalArticle

Cho, H, Yang, YD, Lee, J, Lee, B, Kim, T, Jang, Y, Back, SK, Na, HS, Harfe, BD, Wang, F, Raouf, R, Wood, JN & Oh, U 2012, 'The calcium-activated chloride channel anoctamin 1 acts as a heat sensor in nociceptive neurons', Nature Neuroscience, vol. 15, no. 7, pp. 1015-1021. https://doi.org/10.1038/nn.3111
Cho, Hawon ; Yang, Young Duk ; Lee, Jesun ; Lee, Byeongjoon ; Kim, Tahnbee ; Jang, Yongwoo ; Back, Seung Keun ; Na, Heung Sik ; Harfe, Brian D. ; Wang, Fan ; Raouf, Ramin ; Wood, John N. ; Oh, Uhtaek. / The calcium-activated chloride channel anoctamin 1 acts as a heat sensor in nociceptive neurons. In: Nature Neuroscience. 2012 ; Vol. 15, No. 7. pp. 1015-1021.
@article{ebfc237f6dfc4551b28cc43227de46e2,
title = "The calcium-activated chloride channel anoctamin 1 acts as a heat sensor in nociceptive neurons",
abstract = "Nociceptors are a subset of small primary afferent neurons that respond to noxious chemical, thermal and mechanical stimuli. Ion channels in nociceptors respond differently to noxious stimuli and generate electrical signals in different ways. Anoctamin 1 (ANO1 also known as TMEM16A) is a Ca 2+-activated chloride channel that is essential for numerous physiological functions. We found that ANO1 was activated by temperatures over 44 °C with steep heat sensitivity. ANO1 was expressed in small sensory neurons and was highly colocalized with nociceptor markers, which suggests that it may be involved in nociception. Application of heat ramps to dorsal root ganglion (DRG) neurons elicited robust ANO1-dependent depolarization. Furthermore, knockdown or deletion of ANO1 in DRG neurons substantially reduced nociceptive behavior in thermal pain models. These results indicate that ANO1 is a heat sensor that detects nociceptive thermal stimuli in sensory neurons and possibly mediates nociception.",
author = "Hawon Cho and Yang, {Young Duk} and Jesun Lee and Byeongjoon Lee and Tahnbee Kim and Yongwoo Jang and Back, {Seung Keun} and Na, {Heung Sik} and Harfe, {Brian D.} and Fan Wang and Ramin Raouf and Wood, {John N.} and Uhtaek Oh",
year = "2012",
month = "7",
day = "1",
doi = "10.1038/nn.3111",
language = "English",
volume = "15",
pages = "1015--1021",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - The calcium-activated chloride channel anoctamin 1 acts as a heat sensor in nociceptive neurons

AU - Cho, Hawon

AU - Yang, Young Duk

AU - Lee, Jesun

AU - Lee, Byeongjoon

AU - Kim, Tahnbee

AU - Jang, Yongwoo

AU - Back, Seung Keun

AU - Na, Heung Sik

AU - Harfe, Brian D.

AU - Wang, Fan

AU - Raouf, Ramin

AU - Wood, John N.

AU - Oh, Uhtaek

PY - 2012/7/1

Y1 - 2012/7/1

N2 - Nociceptors are a subset of small primary afferent neurons that respond to noxious chemical, thermal and mechanical stimuli. Ion channels in nociceptors respond differently to noxious stimuli and generate electrical signals in different ways. Anoctamin 1 (ANO1 also known as TMEM16A) is a Ca 2+-activated chloride channel that is essential for numerous physiological functions. We found that ANO1 was activated by temperatures over 44 °C with steep heat sensitivity. ANO1 was expressed in small sensory neurons and was highly colocalized with nociceptor markers, which suggests that it may be involved in nociception. Application of heat ramps to dorsal root ganglion (DRG) neurons elicited robust ANO1-dependent depolarization. Furthermore, knockdown or deletion of ANO1 in DRG neurons substantially reduced nociceptive behavior in thermal pain models. These results indicate that ANO1 is a heat sensor that detects nociceptive thermal stimuli in sensory neurons and possibly mediates nociception.

AB - Nociceptors are a subset of small primary afferent neurons that respond to noxious chemical, thermal and mechanical stimuli. Ion channels in nociceptors respond differently to noxious stimuli and generate electrical signals in different ways. Anoctamin 1 (ANO1 also known as TMEM16A) is a Ca 2+-activated chloride channel that is essential for numerous physiological functions. We found that ANO1 was activated by temperatures over 44 °C with steep heat sensitivity. ANO1 was expressed in small sensory neurons and was highly colocalized with nociceptor markers, which suggests that it may be involved in nociception. Application of heat ramps to dorsal root ganglion (DRG) neurons elicited robust ANO1-dependent depolarization. Furthermore, knockdown or deletion of ANO1 in DRG neurons substantially reduced nociceptive behavior in thermal pain models. These results indicate that ANO1 is a heat sensor that detects nociceptive thermal stimuli in sensory neurons and possibly mediates nociception.

UR - http://www.scopus.com/inward/record.url?scp=84862896642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862896642&partnerID=8YFLogxK

U2 - 10.1038/nn.3111

DO - 10.1038/nn.3111

M3 - Article

C2 - 22634729

AN - SCOPUS:84862896642

VL - 15

SP - 1015

EP - 1021

JO - Nature Neuroscience

JF - Nature Neuroscience

SN - 1097-6256

IS - 7

ER -