The cardioprotective effect of microemulsion propofol against ischemia and reperfusion injury in isolated rat heart

Min Jung Hur, Heezoo Kim, Dong Kyu Lee, Sang Ho Lim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Lipid-emulsion propofol (LP) has cardioprotective effects against ischemia-reperfusion injury, but it has lipid-related side effects. Microemulsion propofol (MP) is a lipid-free propofol emulsified with 10% purified poloxamer 188 (PP188). PP188 is a nonionic surfactant and has cardioprotective effects. However, some reports have suggested that reduced cardioprotective effects were observed when the cardioprotective agents were used in combination even though each cardioprotective agent has cardioprotective effects. The aims of this study were to examine and compare the cardioprotective effects of MP and LP. Methods: 50 isolated rat hearts were perfused with modified Kreb's solution. They were divided into 4 groups and underwent 30 minutes of ischemia and 60 minutes of reperfusion. Control group: ischemia-reperfusion was performed without treatment. LP, MP and PP groups: LP, MP and PP188 were infused during the pre-ischemic and reperfusion period, respectively. Hemodynamic parameters and coronary effluent flow rate (CEFR) were measured. Infarct size was determined using triphenyl-tetrazolium staining. Results: In the MP group, systolic pressure was maintained near baseline, the systolic pressure was higher than that in the other groups and HR was lower than that in the other groups during reperfusion. Diastolic pressure was transiently increased in the PP group after treatment and at 5 minutes after reperfusion compared with that in the control group and in the the LP group. There were no differences in dP/dt max and CEFR between groups. Infarct size in the LP, MP and PP groups was smaller than that in the control group, but there were no significant differences between these three groups. Conclusions: MP has cardioprotective effects similar to those of LP. MP can be used for cardiac anesthesia in cases with ischemia-reperfusion injury to avoid the lipid-related side effects of LP.

Original languageEnglish
Pages (from-to)358-364
Number of pages7
JournalKorean Journal of Anesthesiology
Volume62
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

Fingerprint

Propofol
Reperfusion Injury
Emulsions
Lipids
Reperfusion
Poloxamer
Cardiotonic Agents
Blood Pressure
Control Groups
Ischemia
Surface-Active Agents

Keywords

  • Heart
  • Ischemia
  • Microemulsion propofol
  • Reperfusion injury

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

The cardioprotective effect of microemulsion propofol against ischemia and reperfusion injury in isolated rat heart. / Hur, Min Jung; Kim, Heezoo; Lee, Dong Kyu; Lim, Sang Ho.

In: Korean Journal of Anesthesiology, Vol. 62, No. 4, 01.04.2012, p. 358-364.

Research output: Contribution to journalArticle

@article{fd4abe8a1740485b9605be0ddb24ea39,
title = "The cardioprotective effect of microemulsion propofol against ischemia and reperfusion injury in isolated rat heart",
abstract = "Background: Lipid-emulsion propofol (LP) has cardioprotective effects against ischemia-reperfusion injury, but it has lipid-related side effects. Microemulsion propofol (MP) is a lipid-free propofol emulsified with 10{\%} purified poloxamer 188 (PP188). PP188 is a nonionic surfactant and has cardioprotective effects. However, some reports have suggested that reduced cardioprotective effects were observed when the cardioprotective agents were used in combination even though each cardioprotective agent has cardioprotective effects. The aims of this study were to examine and compare the cardioprotective effects of MP and LP. Methods: 50 isolated rat hearts were perfused with modified Kreb's solution. They were divided into 4 groups and underwent 30 minutes of ischemia and 60 minutes of reperfusion. Control group: ischemia-reperfusion was performed without treatment. LP, MP and PP groups: LP, MP and PP188 were infused during the pre-ischemic and reperfusion period, respectively. Hemodynamic parameters and coronary effluent flow rate (CEFR) were measured. Infarct size was determined using triphenyl-tetrazolium staining. Results: In the MP group, systolic pressure was maintained near baseline, the systolic pressure was higher than that in the other groups and HR was lower than that in the other groups during reperfusion. Diastolic pressure was transiently increased in the PP group after treatment and at 5 minutes after reperfusion compared with that in the control group and in the the LP group. There were no differences in dP/dt max and CEFR between groups. Infarct size in the LP, MP and PP groups was smaller than that in the control group, but there were no significant differences between these three groups. Conclusions: MP has cardioprotective effects similar to those of LP. MP can be used for cardiac anesthesia in cases with ischemia-reperfusion injury to avoid the lipid-related side effects of LP.",
keywords = "Heart, Ischemia, Microemulsion propofol, Reperfusion injury",
author = "Hur, {Min Jung} and Heezoo Kim and Lee, {Dong Kyu} and Lim, {Sang Ho}",
year = "2012",
month = "4",
day = "1",
doi = "10.4097/kjae.2012.62.4.358",
language = "English",
volume = "62",
pages = "358--364",
journal = "Korean Journal of Anesthesiology",
issn = "2005-6419",
publisher = "Korean Society of Anesthesiologists",
number = "4",

}

TY - JOUR

T1 - The cardioprotective effect of microemulsion propofol against ischemia and reperfusion injury in isolated rat heart

AU - Hur, Min Jung

AU - Kim, Heezoo

AU - Lee, Dong Kyu

AU - Lim, Sang Ho

PY - 2012/4/1

Y1 - 2012/4/1

N2 - Background: Lipid-emulsion propofol (LP) has cardioprotective effects against ischemia-reperfusion injury, but it has lipid-related side effects. Microemulsion propofol (MP) is a lipid-free propofol emulsified with 10% purified poloxamer 188 (PP188). PP188 is a nonionic surfactant and has cardioprotective effects. However, some reports have suggested that reduced cardioprotective effects were observed when the cardioprotective agents were used in combination even though each cardioprotective agent has cardioprotective effects. The aims of this study were to examine and compare the cardioprotective effects of MP and LP. Methods: 50 isolated rat hearts were perfused with modified Kreb's solution. They were divided into 4 groups and underwent 30 minutes of ischemia and 60 minutes of reperfusion. Control group: ischemia-reperfusion was performed without treatment. LP, MP and PP groups: LP, MP and PP188 were infused during the pre-ischemic and reperfusion period, respectively. Hemodynamic parameters and coronary effluent flow rate (CEFR) were measured. Infarct size was determined using triphenyl-tetrazolium staining. Results: In the MP group, systolic pressure was maintained near baseline, the systolic pressure was higher than that in the other groups and HR was lower than that in the other groups during reperfusion. Diastolic pressure was transiently increased in the PP group after treatment and at 5 minutes after reperfusion compared with that in the control group and in the the LP group. There were no differences in dP/dt max and CEFR between groups. Infarct size in the LP, MP and PP groups was smaller than that in the control group, but there were no significant differences between these three groups. Conclusions: MP has cardioprotective effects similar to those of LP. MP can be used for cardiac anesthesia in cases with ischemia-reperfusion injury to avoid the lipid-related side effects of LP.

AB - Background: Lipid-emulsion propofol (LP) has cardioprotective effects against ischemia-reperfusion injury, but it has lipid-related side effects. Microemulsion propofol (MP) is a lipid-free propofol emulsified with 10% purified poloxamer 188 (PP188). PP188 is a nonionic surfactant and has cardioprotective effects. However, some reports have suggested that reduced cardioprotective effects were observed when the cardioprotective agents were used in combination even though each cardioprotective agent has cardioprotective effects. The aims of this study were to examine and compare the cardioprotective effects of MP and LP. Methods: 50 isolated rat hearts were perfused with modified Kreb's solution. They were divided into 4 groups and underwent 30 minutes of ischemia and 60 minutes of reperfusion. Control group: ischemia-reperfusion was performed without treatment. LP, MP and PP groups: LP, MP and PP188 were infused during the pre-ischemic and reperfusion period, respectively. Hemodynamic parameters and coronary effluent flow rate (CEFR) were measured. Infarct size was determined using triphenyl-tetrazolium staining. Results: In the MP group, systolic pressure was maintained near baseline, the systolic pressure was higher than that in the other groups and HR was lower than that in the other groups during reperfusion. Diastolic pressure was transiently increased in the PP group after treatment and at 5 minutes after reperfusion compared with that in the control group and in the the LP group. There were no differences in dP/dt max and CEFR between groups. Infarct size in the LP, MP and PP groups was smaller than that in the control group, but there were no significant differences between these three groups. Conclusions: MP has cardioprotective effects similar to those of LP. MP can be used for cardiac anesthesia in cases with ischemia-reperfusion injury to avoid the lipid-related side effects of LP.

KW - Heart

KW - Ischemia

KW - Microemulsion propofol

KW - Reperfusion injury

UR - http://www.scopus.com/inward/record.url?scp=84860289749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860289749&partnerID=8YFLogxK

U2 - 10.4097/kjae.2012.62.4.358

DO - 10.4097/kjae.2012.62.4.358

M3 - Article

VL - 62

SP - 358

EP - 364

JO - Korean Journal of Anesthesiology

JF - Korean Journal of Anesthesiology

SN - 2005-6419

IS - 4

ER -