The CID1 cyclin C-like gene is important for plant infection in Fusarium graminearum

Xiaoying Zhou, Christina Heyer, Yoon-E Choi, Rahim Mehrabi, Jin Rong Xu

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Head blight or scab caused by Fusarium graminearum is a destructive disease of wheat and barley. The pathogen can cause severe yield losses and contaminates infested kernels with harmful mycotoxins. In this study, we characterized the CID1 gene in F. graminearum that is an ortholog of the Fusarium verticilloides FCC1 and yeast UME3 genes. The protein encoded by CID1 has typical structural features of C-type cyclins. Deletion of CID1 resulted in a reduction in conidiation and vegetative growth but an increase in pigmentation. The Δcid1 mutant was female sterile but could outcross as a male. It was significantly reduced in DON production and virulence on wheat heads and corn stalks. Only about 50% of inoculated spikelets developed scab symptoms and scab disease rarely extended to nearby florets, suggesting that the Δcid1 mutant was defective in colonizing and spreading in wheat heads. Deletion of CID1 resulted in reduced expression levels of TRI5 and TRI101 but increased PKS12 expression. When expressed in F. verticillioides, the CID1 gene complemented the defects of the Δfcc1 mutant in conidiation, hyphal growth, and fumonisin production. Our data indicate that the CID1 C-type cyclin gene plays multiple roles in the regulation of vegetative growth, sexual development, conidiation, DON production, and pathogenicity.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalFungal Genetics and Biology
Volume47
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1
Externally publishedYes

Fingerprint

Cyclin C
Fusariosis
Triticum
Fusarium
Genes
Virulence
Head
Fumonisins
Sexual Development
Mycotoxins
Pigmentation
Hordeum
Growth
Growth and Development
Zea mays
Yeasts
Proteins

Keywords

  • Deoxynivalenol
  • Gibberella zeae
  • Mycotoxin
  • Pathogenicity

ASJC Scopus subject areas

  • Microbiology
  • Genetics

Cite this

The CID1 cyclin C-like gene is important for plant infection in Fusarium graminearum. / Zhou, Xiaoying; Heyer, Christina; Choi, Yoon-E; Mehrabi, Rahim; Xu, Jin Rong.

In: Fungal Genetics and Biology, Vol. 47, No. 2, 01.02.2010, p. 143-151.

Research output: Contribution to journalArticle

Zhou, Xiaoying ; Heyer, Christina ; Choi, Yoon-E ; Mehrabi, Rahim ; Xu, Jin Rong. / The CID1 cyclin C-like gene is important for plant infection in Fusarium graminearum. In: Fungal Genetics and Biology. 2010 ; Vol. 47, No. 2. pp. 143-151.
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