The complete enzymatic saccharification of agarose and its application to simultaneous saccharification and fermentation of agarose for ethanol production

Hee Taek Kim, Saeyoung Lee, Kyoung Heon Kim, In Geol Choi

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

A sugar platform equipped with acetic acid, multiple agarases and neoagarobiose hydrolase (NABH) converted recalcitrant agar polysaccharide into monosugars, which was evaluated by simultaneous saccharification and fermentation (SSF). The sugar platform was divided into chemical liquefaction and enzymatic saccharification. The chemical liquefaction was carried out in mild conditions (using a dilute acetic acid at 80 °C for 1-6. h) to avoid the production of fermentation inhibitors and hence the highest degree of liquefaction of 95.6% (w/w) was obtained. We mimicked the natural agarolytic pathway using three microbial agarases (Aga16B, Aga50D and DagA) and NABH, and the enzyme system converted 79.1% of agarose to monosugars. The chemical liquefaction and SSF of 30. g/l agarose resulted in 4.4. g/l ethanol concentration and 49.3% of the theoretical ethanol yield to d-galactose. This is the first report on the complete enzymatic conversion of agarose into its monosugars and the SSF of agarose into ethanol.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalBioresource technology
Volume107
DOIs
Publication statusPublished - 2012 Mar

Keywords

  • Agarose
  • Chemical liquefaction
  • Enzymatic saccharification
  • Fermentation
  • Simultaneous saccharification

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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