The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism

In Su Park, Min Han, Jong Won Rhie, Soo Hyun Kim, Youngmee Jung, Ik Hwan Kim, Sang Heon Kim

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

In recent years, research in the areas of stem cells has dramatically increased, including studies of cellular adhesion to a substrate. We sought to determine the adhesive properties of human adipose-derived stem cells (hASCs) for extracellular matrix proteins. The adhesion of hASCs to collagens and laminin was completely inhibited by a monoclonal antibody, Mab 2253, which binds to the β1 integrin subunit. These data indicate that hASC adhesion to collagens and laminin was exclusively mediated by an integrin. Cell adhesion on fibronectin (Fn) was inhibited by the heparin-binding peptide (HBP) in the presence of Mab 2253, but not by either Mab 2253 or HBP alone. These results indicate that both the β1 subunit and the heparan sulfate proteoglycan participated in the cell adhesion to Fn. Microscopic views showed extensive spreading of hASCs cultured on Fn, whereas the cells maintained a round shape when cultured on a heparin-binding domain (HBD) substrate. hASCs differentiated into adipocytes, which stained positive for lipid vacuoles by Oil Red-O analysis, more readily on HBD substrate than on FN substrate. These results suggest that hASCs have an adhesion mechanism for the HBD of Fn and hASC morphology is controlled by the adhesion mechanism and strongly correlated with adipogenic differentiation.

Original languageEnglish
Pages (from-to)6835-6843
Number of pages9
JournalBiomaterials
Volume30
Issue number36
DOIs
Publication statusPublished - 2009 Dec 1

Fingerprint

Cell adhesion
Stem cells
Cell Adhesion
Cell Differentiation
Stem Cells
Heparin
Fibronectins
Adhesion
Laminin
Substrates
Collagen
Integrins
Peptides
Heparan Sulfate Proteoglycans
Monoclonal antibodies
Extracellular Matrix Proteins
Vacuoles
Adipocytes
Adhesives
Lipids

Keywords

  • Cell adhesion substrate
  • Cell differentiation
  • Extracellular matrix
  • Fibronectin
  • Heparin-binding domain
  • Human adipose-derived stem cells

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism. / Park, In Su; Han, Min; Rhie, Jong Won; Kim, Soo Hyun; Jung, Youngmee; Kim, Ik Hwan; Kim, Sang Heon.

In: Biomaterials, Vol. 30, No. 36, 01.12.2009, p. 6835-6843.

Research output: Contribution to journalArticle

Park, In Su ; Han, Min ; Rhie, Jong Won ; Kim, Soo Hyun ; Jung, Youngmee ; Kim, Ik Hwan ; Kim, Sang Heon. / The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism. In: Biomaterials. 2009 ; Vol. 30, No. 36. pp. 6835-6843.
@article{056e1f14ccb2456e8b8214a9d8fdfe3e,
title = "The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism",
abstract = "In recent years, research in the areas of stem cells has dramatically increased, including studies of cellular adhesion to a substrate. We sought to determine the adhesive properties of human adipose-derived stem cells (hASCs) for extracellular matrix proteins. The adhesion of hASCs to collagens and laminin was completely inhibited by a monoclonal antibody, Mab 2253, which binds to the β1 integrin subunit. These data indicate that hASC adhesion to collagens and laminin was exclusively mediated by an integrin. Cell adhesion on fibronectin (Fn) was inhibited by the heparin-binding peptide (HBP) in the presence of Mab 2253, but not by either Mab 2253 or HBP alone. These results indicate that both the β1 subunit and the heparan sulfate proteoglycan participated in the cell adhesion to Fn. Microscopic views showed extensive spreading of hASCs cultured on Fn, whereas the cells maintained a round shape when cultured on a heparin-binding domain (HBD) substrate. hASCs differentiated into adipocytes, which stained positive for lipid vacuoles by Oil Red-O analysis, more readily on HBD substrate than on FN substrate. These results suggest that hASCs have an adhesion mechanism for the HBD of Fn and hASC morphology is controlled by the adhesion mechanism and strongly correlated with adipogenic differentiation.",
keywords = "Cell adhesion substrate, Cell differentiation, Extracellular matrix, Fibronectin, Heparin-binding domain, Human adipose-derived stem cells",
author = "Park, {In Su} and Min Han and Rhie, {Jong Won} and Kim, {Soo Hyun} and Youngmee Jung and Kim, {Ik Hwan} and Kim, {Sang Heon}",
year = "2009",
month = "12",
day = "1",
doi = "10.1016/j.biomaterials.2009.08.057",
language = "English",
volume = "30",
pages = "6835--6843",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "36",

}

TY - JOUR

T1 - The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism

AU - Park, In Su

AU - Han, Min

AU - Rhie, Jong Won

AU - Kim, Soo Hyun

AU - Jung, Youngmee

AU - Kim, Ik Hwan

AU - Kim, Sang Heon

PY - 2009/12/1

Y1 - 2009/12/1

N2 - In recent years, research in the areas of stem cells has dramatically increased, including studies of cellular adhesion to a substrate. We sought to determine the adhesive properties of human adipose-derived stem cells (hASCs) for extracellular matrix proteins. The adhesion of hASCs to collagens and laminin was completely inhibited by a monoclonal antibody, Mab 2253, which binds to the β1 integrin subunit. These data indicate that hASC adhesion to collagens and laminin was exclusively mediated by an integrin. Cell adhesion on fibronectin (Fn) was inhibited by the heparin-binding peptide (HBP) in the presence of Mab 2253, but not by either Mab 2253 or HBP alone. These results indicate that both the β1 subunit and the heparan sulfate proteoglycan participated in the cell adhesion to Fn. Microscopic views showed extensive spreading of hASCs cultured on Fn, whereas the cells maintained a round shape when cultured on a heparin-binding domain (HBD) substrate. hASCs differentiated into adipocytes, which stained positive for lipid vacuoles by Oil Red-O analysis, more readily on HBD substrate than on FN substrate. These results suggest that hASCs have an adhesion mechanism for the HBD of Fn and hASC morphology is controlled by the adhesion mechanism and strongly correlated with adipogenic differentiation.

AB - In recent years, research in the areas of stem cells has dramatically increased, including studies of cellular adhesion to a substrate. We sought to determine the adhesive properties of human adipose-derived stem cells (hASCs) for extracellular matrix proteins. The adhesion of hASCs to collagens and laminin was completely inhibited by a monoclonal antibody, Mab 2253, which binds to the β1 integrin subunit. These data indicate that hASC adhesion to collagens and laminin was exclusively mediated by an integrin. Cell adhesion on fibronectin (Fn) was inhibited by the heparin-binding peptide (HBP) in the presence of Mab 2253, but not by either Mab 2253 or HBP alone. These results indicate that both the β1 subunit and the heparan sulfate proteoglycan participated in the cell adhesion to Fn. Microscopic views showed extensive spreading of hASCs cultured on Fn, whereas the cells maintained a round shape when cultured on a heparin-binding domain (HBD) substrate. hASCs differentiated into adipocytes, which stained positive for lipid vacuoles by Oil Red-O analysis, more readily on HBD substrate than on FN substrate. These results suggest that hASCs have an adhesion mechanism for the HBD of Fn and hASC morphology is controlled by the adhesion mechanism and strongly correlated with adipogenic differentiation.

KW - Cell adhesion substrate

KW - Cell differentiation

KW - Extracellular matrix

KW - Fibronectin

KW - Heparin-binding domain

KW - Human adipose-derived stem cells

UR - http://www.scopus.com/inward/record.url?scp=70350346591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350346591&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2009.08.057

DO - 10.1016/j.biomaterials.2009.08.057

M3 - Article

C2 - 19781767

AN - SCOPUS:70350346591

VL - 30

SP - 6835

EP - 6843

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 36

ER -