The crystal structure of flap endonuclease-1 from Methanococcus jannaschii

Kwang Yeon Hwang, Kyuwon Baek, Hye Yeon Kim, Yunje Cho

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Å resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.

Original languageEnglish
Pages (from-to)707-713
Number of pages7
JournalNature Structural Biology
Volume5
Issue number8
DOIs
Publication statusPublished - 1998 Aug 1
Externally publishedYes

Fingerprint

Flap Endonucleases
Methanocaldococcus
Crystal structure
DNA
Catalytic Domain
Sequence Deletion
DNA Replication
DNA Repair
Metal ions
Repair
Metals
Ions
Substrates
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

The crystal structure of flap endonuclease-1 from Methanococcus jannaschii. / Hwang, Kwang Yeon; Baek, Kyuwon; Kim, Hye Yeon; Cho, Yunje.

In: Nature Structural Biology, Vol. 5, No. 8, 01.08.1998, p. 707-713.

Research output: Contribution to journalArticle

Hwang, Kwang Yeon ; Baek, Kyuwon ; Kim, Hye Yeon ; Cho, Yunje. / The crystal structure of flap endonuclease-1 from Methanococcus jannaschii. In: Nature Structural Biology. 1998 ; Vol. 5, No. 8. pp. 707-713.
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