The crystal structure of flap endonuclease-1 from Methanococcus jannaschii

Kwang Yeon Hwang, Kyuwon Baek, Hye Yeon Kim, Yunje Cho

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Abstract

Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Å resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.

Original languageEnglish
Pages (from-to)707-713
Number of pages7
JournalNature Structural Biology
Volume5
Issue number8
DOIs
Publication statusPublished - 1998 Aug 1
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

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