Abstract
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Å resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 707-713 |
| Number of pages | 7 |
| Journal | Nature Structural Biology |
| Volume | 5 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 1998 Aug 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Structural Biology
- Genetics
Cite this
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii. / Hwang, Kwang Yeon; Baek, Kyuwon; Kim, Hye Yeon; Cho, Yunje.
In: Nature Structural Biology, Vol. 5, No. 8, 01.08.1998, p. 707-713.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The crystal structure of flap endonuclease-1 from Methanococcus jannaschii
AU - Hwang, Kwang Yeon
AU - Baek, Kyuwon
AU - Kim, Hye Yeon
AU - Cho, Yunje
PY - 1998/8/1
Y1 - 1998/8/1
N2 - Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Å resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
AB - Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Å resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
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U2 - 10.1038/1406
DO - 10.1038/1406
M3 - Article
C2 - 9699635
AN - SCOPUS:0031873411
VL - 5
SP - 707
EP - 713
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
SN - 1545-9993
IS - 8
ER -