The cytosolic splicing variant of NELL2 inhibits PKCβ1 in glial cells

Da Yong Lee, Eunju Kim, Young Sun Lee, Hwani Ryu, Jae-Yong Park, Eun Mi Hwang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

NELL2 is an abundant glycoprotein containing EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-specific variant and the other is cytosolic NELL2 (cNELL2) which is non-secreted splicing variant of NELL2. Although cNELL2 structure was well characterized, the expression pattern or the cellular function of cNELL2 is not fully determined. In this study, we found that cNELL2 specifically interacts with PKCβ isotypes and inhibits PKCβ1 through direct binding to the N-terminal pseudosubstrate domain of PKCβ1. Here, we also demonstrate that cNELL2 is predominantly expressed and has inhibitory effects on the PKC downstream signaling pathways in astrocytes thereby establishing cNELL2 as an endogenous inhibitor of PKCβ1 in glia.

Original languageEnglish
Pages (from-to)459-464
Number of pages6
JournalBiochemical and biophysical research communications
Volume454
Issue number3
DOIs
Publication statusPublished - 2014 Nov 21

Fingerprint

Neuroglia
Protein Kinase C
Epidermal Growth Factor
Astrocytes
Neurons
Glycoproteins
Tissue

Keywords

  • Astrocytes
  • Cytosolic NELL2
  • NELL2
  • PKCβ1
  • Pseudosubstrate domain

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The cytosolic splicing variant of NELL2 inhibits PKCβ1 in glial cells. / Lee, Da Yong; Kim, Eunju; Lee, Young Sun; Ryu, Hwani; Park, Jae-Yong; Hwang, Eun Mi.

In: Biochemical and biophysical research communications, Vol. 454, No. 3, 21.11.2014, p. 459-464.

Research output: Contribution to journalArticle

Lee, Da Yong ; Kim, Eunju ; Lee, Young Sun ; Ryu, Hwani ; Park, Jae-Yong ; Hwang, Eun Mi. / The cytosolic splicing variant of NELL2 inhibits PKCβ1 in glial cells. In: Biochemical and biophysical research communications. 2014 ; Vol. 454, No. 3. pp. 459-464.
@article{f5a7533a5c34427e88f289803fae7738,
title = "The cytosolic splicing variant of NELL2 inhibits PKCβ1 in glial cells",
abstract = "NELL2 is an abundant glycoprotein containing EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-specific variant and the other is cytosolic NELL2 (cNELL2) which is non-secreted splicing variant of NELL2. Although cNELL2 structure was well characterized, the expression pattern or the cellular function of cNELL2 is not fully determined. In this study, we found that cNELL2 specifically interacts with PKCβ isotypes and inhibits PKCβ1 through direct binding to the N-terminal pseudosubstrate domain of PKCβ1. Here, we also demonstrate that cNELL2 is predominantly expressed and has inhibitory effects on the PKC downstream signaling pathways in astrocytes thereby establishing cNELL2 as an endogenous inhibitor of PKCβ1 in glia.",
keywords = "Astrocytes, Cytosolic NELL2, NELL2, PKCβ1, Pseudosubstrate domain",
author = "Lee, {Da Yong} and Eunju Kim and Lee, {Young Sun} and Hwani Ryu and Jae-Yong Park and Hwang, {Eun Mi}",
year = "2014",
month = "11",
day = "21",
doi = "10.1016/j.bbrc.2014.10.110",
language = "English",
volume = "454",
pages = "459--464",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - The cytosolic splicing variant of NELL2 inhibits PKCβ1 in glial cells

AU - Lee, Da Yong

AU - Kim, Eunju

AU - Lee, Young Sun

AU - Ryu, Hwani

AU - Park, Jae-Yong

AU - Hwang, Eun Mi

PY - 2014/11/21

Y1 - 2014/11/21

N2 - NELL2 is an abundant glycoprotein containing EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-specific variant and the other is cytosolic NELL2 (cNELL2) which is non-secreted splicing variant of NELL2. Although cNELL2 structure was well characterized, the expression pattern or the cellular function of cNELL2 is not fully determined. In this study, we found that cNELL2 specifically interacts with PKCβ isotypes and inhibits PKCβ1 through direct binding to the N-terminal pseudosubstrate domain of PKCβ1. Here, we also demonstrate that cNELL2 is predominantly expressed and has inhibitory effects on the PKC downstream signaling pathways in astrocytes thereby establishing cNELL2 as an endogenous inhibitor of PKCβ1 in glia.

AB - NELL2 is an abundant glycoprotein containing EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-specific variant and the other is cytosolic NELL2 (cNELL2) which is non-secreted splicing variant of NELL2. Although cNELL2 structure was well characterized, the expression pattern or the cellular function of cNELL2 is not fully determined. In this study, we found that cNELL2 specifically interacts with PKCβ isotypes and inhibits PKCβ1 through direct binding to the N-terminal pseudosubstrate domain of PKCβ1. Here, we also demonstrate that cNELL2 is predominantly expressed and has inhibitory effects on the PKC downstream signaling pathways in astrocytes thereby establishing cNELL2 as an endogenous inhibitor of PKCβ1 in glia.

KW - Astrocytes

KW - Cytosolic NELL2

KW - NELL2

KW - PKCβ1

KW - Pseudosubstrate domain

UR - http://www.scopus.com/inward/record.url?scp=84910112554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84910112554&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2014.10.110

DO - 10.1016/j.bbrc.2014.10.110

M3 - Article

VL - 454

SP - 459

EP - 464

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -