The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates

Gang Il Kim, Kyung Woo Kim, Min-Kyu Oh, Yun Mo Sung

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

High-sensitivity, high-specificity detection of platelet derived growth factor (PDGF)-BB was realized using the change in fluorescence resonance energy transfer (FRET) occurring between quantum dot (QD) donors and black hole quencher (BHQ) acceptors. CdSe/ZnS QD/mercaptoacetic acid (MAA)/PDGF aptamer bioconjugates were successfully synthesized using ligand exchange. Black hole quencher (BHQ)-bearing oligonucleotide molecules showing partial sequence matching to PDGF aptamer were attached to PDGF aptamers and photoluminescence (PL) quenching was obtained through FRET. By adding target PDGF-BB to the bioconjugates containing BHQs, PL recovery was detected due to detachment of BHQ-bearing oligonucleotide from the PDGF aptamer as a result of the difference in affinity to the PDGF aptamer. The detection limit of the sensor was ∼0.4nM and the linearity was maintained up to 1.6nM in the PL intensity versus concentration curve. Measurement of PL recovery was suggested as a strong tool for high-sensitivity detection of PDGF-BB. Epidermal growth factor (EGF), the negative control molecule, did not contribute to PL recovery due to lack of binding affinity to the PDGF aptamers, which demonstrates the selectivity of the biosensor.

Original languageEnglish
Article number175503
JournalNanotechnology
Volume20
Issue number17
DOIs
Publication statusPublished - 2009 May 18

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Quantum Dots
Oligonucleotides
Platelet-Derived Growth Factor
Platelets
Semiconductor quantum dots
Photoluminescence
Bearings (structural)
Fluorescence Resonance Energy Transfer
Recovery
Molecules
Biosensing Techniques
Epidermal Growth Factor
Biosensors
Limit of Detection
Intercellular Signaling Peptides and Proteins
Quenching
Ligands
Sensitivity and Specificity
Sensors
platelet-derived growth factor BB

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates. / Kim, Gang Il; Kim, Kyung Woo; Oh, Min-Kyu; Sung, Yun Mo.

In: Nanotechnology, Vol. 20, No. 17, 175503, 18.05.2009.

Research output: Contribution to journalArticle

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