The discovery of biomarkers for type 2 diabetic nephropathy by serum proteome analysis

Eun Hee Cho, Mi Ryung Kim, Hyun Jung Kim, Do Yeon Lee, Pan Kyeom Kim, Kyung Mook Choi, Ohk Hyun Ryu, Chan Wha Kim

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Diabetic nephropathy (DN) is a serious kidney complication of diabetes, and constitutes the leading cause of end-stage renal disease. The earliest clinical evidence of DN is microalbuminuria, a term which refers to the appearance of small but abnormal amounts of albumin in the urine. However, screening methods for DN, such as biomarker assays, are yet to be developed for type 2 DN. In the present study, in an attempt to identify the biomarkers for initial diagnoses of type 2 DN, the protein profiles of human sera collected from 30 microalbuminuric type 2 diabetic patients were compared with those collected from 30 normoalbuminuric type 2 diabetic patients, via 2-DE. As a result, a total of 18 spots were determined to have different protein levels in the microalbuminuric patients. Twelve spots had lower protein levels of approximately 50%, and the other six had higher levels of approximately 100-300% as compared to the spots of normoalbuminuric patients. These spots were identified with ESI-Q-TOF (ESI-quadrupole-TOF) MS. Among the identified proteins, vitamin D-binding protein (DBP) and pigment epithelium-derived factor (PEDF) were verified by Western blotting. The results of this study indicate that the DBP may be employed as diagnostic and monitoring biomarkers of type 2 DN, contingent on further study into the matter.

Original languageEnglish
Pages (from-to)352-361
Number of pages10
JournalProteomics - Clinical Applications
Volume1
Issue number4
DOIs
Publication statusPublished - 2007 Apr

Keywords

  • 2-DE
  • Biomarker
  • Diabetic nephropathy
  • Microalbuminuria
  • Vitamin D-binding protein

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Cho, E. H., Kim, M. R., Kim, H. J., Lee, D. Y., Kim, P. K., Choi, K. M., Ryu, O. H., & Kim, C. W. (2007). The discovery of biomarkers for type 2 diabetic nephropathy by serum proteome analysis. Proteomics - Clinical Applications, 1(4), 352-361. https://doi.org/10.1002/prca.200600608