The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis

Hyung Sae Kim, Sung Jin Kim, Nazia Abbasi, Ray A. Bressan, Dae Jin Yun, Sang-Dong Yoo, Suk Yun Kwon, Sang Bong Choi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Dof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxialabaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxialabaxial polarity.

Original languageEnglish
Pages (from-to)524-535
Number of pages12
JournalPlant Journal
Volume64
Issue number3
DOIs
Publication statusPublished - 2010 Nov 1
Externally publishedYes

Fingerprint

Arabidopsis
DNA-binding domains
Nucleotide Motifs
Transcription Factors
transcription factors
transcription (genetics)
promoter regions
Phenotype
Genetic Promoter Regions
DNA
leaves
Indoleacetic Acids
phenotype
Chromatin Immunoprecipitation
mutants
Zinc Fingers
Electrophoretic Mobility Shift Assay
assays
Proteins
leaf curling

Keywords

  • Activation tagging mutant
  • Dof transcription factor
  • Dof5.1
  • Leaf polarity
  • Revoluta

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis. / Kim, Hyung Sae; Kim, Sung Jin; Abbasi, Nazia; Bressan, Ray A.; Yun, Dae Jin; Yoo, Sang-Dong; Kwon, Suk Yun; Choi, Sang Bong.

In: Plant Journal, Vol. 64, No. 3, 01.11.2010, p. 524-535.

Research output: Contribution to journalArticle

Kim, Hyung Sae ; Kim, Sung Jin ; Abbasi, Nazia ; Bressan, Ray A. ; Yun, Dae Jin ; Yoo, Sang-Dong ; Kwon, Suk Yun ; Choi, Sang Bong. / The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis. In: Plant Journal. 2010 ; Vol. 64, No. 3. pp. 524-535.
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