The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis

Hyung Sae Kim, Sung Jin Kim, Nazia Abbasi, Ray A. Bressan, Dae Jin Yun, Sang-Dong Yoo, Suk Yun Kwon, Sang Bong Choi

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41 Citations (Scopus)

Abstract

Dof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxialabaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxialabaxial polarity.

Original languageEnglish
Pages (from-to)524-535
Number of pages12
JournalPlant Journal
Volume64
Issue number3
DOIs
Publication statusPublished - 2010 Nov 1
Externally publishedYes

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Keywords

  • Activation tagging mutant
  • Dof transcription factor
  • Dof5.1
  • Leaf polarity
  • Revoluta

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

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