The drug resistance profile of mycobacterium abscessus group strains from korea

Seung Heon Lee, Hee Kyung Yoo, Seol Hee Kim, Won Jung Koh, Chang K. Kim, Young Kil Park, Hee Jin Kim

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background: Bacteria of the Mycobacterium abscessus group are the second most common pathogens responsible for lung disease caused by nontuberculous mycobacteria in Korea. There is still a lack of studies investigating the genetic mechanisms involved in M. abscessus resistance to antibiotics other than clarithromycin. This study investigated the characteristics of drug resistance exhibited by M. abscessus clinical isolates from Korea. Methods: We performed drug susceptibility testing for a total of 404 M. abscessus clinical strains. Subspecies were differentiated by molecular biological methods and examined for mutations in drug resistance-related genes. Results: Of the 404 strains examined, 202 (50.00%), 199 (49.26%), and 3 (0.74%) strains were identified as M. abscessus, M. massiliense, and M. bolletii, respectively. Of the 152 clarithromycin-resistant strains, 6 possessed rrl mutations, while 4 of the 30 amikacin-resistant strains contained rrs mutations, and 5 of the 114 quinolone-resistant strains had gyr mutations. All mutant strains had high minimal inhibitory concentration values for the antibiotics. Conclusions: Our results showed the distribution of the strains with mutations in drug resistance-related genes was low in the M. abscessus group. Furthermore, we performed drug susceptibility testing and sequence analyses to determine the characteristics of these genes in the M. abscessus group.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalAnnals of Laboratory Medicine
Volume34
Issue number1
DOIs
Publication statusPublished - 2014 Feb 5
Externally publishedYes

Fingerprint

Mycobacterium
Korea
Drug Resistance
Mutation
Pharmaceutical Preparations
Clarithromycin
Genes
Anti-Bacterial Agents
Nontuberculous Mycobacteria
Pulmonary diseases
Amikacin
Quinolones
Testing
Pathogens
Microbial Drug Resistance
Lung Diseases
Sequence Analysis
Bacteria

Keywords

  • Drug resistance
  • Mutation
  • Mycobacterium abscessus group

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

The drug resistance profile of mycobacterium abscessus group strains from korea. / Lee, Seung Heon; Yoo, Hee Kyung; Kim, Seol Hee; Koh, Won Jung; Kim, Chang K.; Park, Young Kil; Kim, Hee Jin.

In: Annals of Laboratory Medicine, Vol. 34, No. 1, 05.02.2014, p. 31-37.

Research output: Contribution to journalArticle

Lee, Seung Heon ; Yoo, Hee Kyung ; Kim, Seol Hee ; Koh, Won Jung ; Kim, Chang K. ; Park, Young Kil ; Kim, Hee Jin. / The drug resistance profile of mycobacterium abscessus group strains from korea. In: Annals of Laboratory Medicine. 2014 ; Vol. 34, No. 1. pp. 31-37.
@article{783f1619340b4c8b9d39fa0d190f8aca,
title = "The drug resistance profile of mycobacterium abscessus group strains from korea",
abstract = "Background: Bacteria of the Mycobacterium abscessus group are the second most common pathogens responsible for lung disease caused by nontuberculous mycobacteria in Korea. There is still a lack of studies investigating the genetic mechanisms involved in M. abscessus resistance to antibiotics other than clarithromycin. This study investigated the characteristics of drug resistance exhibited by M. abscessus clinical isolates from Korea. Methods: We performed drug susceptibility testing for a total of 404 M. abscessus clinical strains. Subspecies were differentiated by molecular biological methods and examined for mutations in drug resistance-related genes. Results: Of the 404 strains examined, 202 (50.00{\%}), 199 (49.26{\%}), and 3 (0.74{\%}) strains were identified as M. abscessus, M. massiliense, and M. bolletii, respectively. Of the 152 clarithromycin-resistant strains, 6 possessed rrl mutations, while 4 of the 30 amikacin-resistant strains contained rrs mutations, and 5 of the 114 quinolone-resistant strains had gyr mutations. All mutant strains had high minimal inhibitory concentration values for the antibiotics. Conclusions: Our results showed the distribution of the strains with mutations in drug resistance-related genes was low in the M. abscessus group. Furthermore, we performed drug susceptibility testing and sequence analyses to determine the characteristics of these genes in the M. abscessus group.",
keywords = "Drug resistance, Mutation, Mycobacterium abscessus group",
author = "Lee, {Seung Heon} and Yoo, {Hee Kyung} and Kim, {Seol Hee} and Koh, {Won Jung} and Kim, {Chang K.} and Park, {Young Kil} and Kim, {Hee Jin}",
year = "2014",
month = "2",
day = "5",
doi = "10.3343/alm.2014.34.1.31",
language = "English",
volume = "34",
pages = "31--37",
journal = "Annals of Laboratory Medicine",
issn = "2234-3806",
publisher = "Seoul National University",
number = "1",

}

TY - JOUR

T1 - The drug resistance profile of mycobacterium abscessus group strains from korea

AU - Lee, Seung Heon

AU - Yoo, Hee Kyung

AU - Kim, Seol Hee

AU - Koh, Won Jung

AU - Kim, Chang K.

AU - Park, Young Kil

AU - Kim, Hee Jin

PY - 2014/2/5

Y1 - 2014/2/5

N2 - Background: Bacteria of the Mycobacterium abscessus group are the second most common pathogens responsible for lung disease caused by nontuberculous mycobacteria in Korea. There is still a lack of studies investigating the genetic mechanisms involved in M. abscessus resistance to antibiotics other than clarithromycin. This study investigated the characteristics of drug resistance exhibited by M. abscessus clinical isolates from Korea. Methods: We performed drug susceptibility testing for a total of 404 M. abscessus clinical strains. Subspecies were differentiated by molecular biological methods and examined for mutations in drug resistance-related genes. Results: Of the 404 strains examined, 202 (50.00%), 199 (49.26%), and 3 (0.74%) strains were identified as M. abscessus, M. massiliense, and M. bolletii, respectively. Of the 152 clarithromycin-resistant strains, 6 possessed rrl mutations, while 4 of the 30 amikacin-resistant strains contained rrs mutations, and 5 of the 114 quinolone-resistant strains had gyr mutations. All mutant strains had high minimal inhibitory concentration values for the antibiotics. Conclusions: Our results showed the distribution of the strains with mutations in drug resistance-related genes was low in the M. abscessus group. Furthermore, we performed drug susceptibility testing and sequence analyses to determine the characteristics of these genes in the M. abscessus group.

AB - Background: Bacteria of the Mycobacterium abscessus group are the second most common pathogens responsible for lung disease caused by nontuberculous mycobacteria in Korea. There is still a lack of studies investigating the genetic mechanisms involved in M. abscessus resistance to antibiotics other than clarithromycin. This study investigated the characteristics of drug resistance exhibited by M. abscessus clinical isolates from Korea. Methods: We performed drug susceptibility testing for a total of 404 M. abscessus clinical strains. Subspecies were differentiated by molecular biological methods and examined for mutations in drug resistance-related genes. Results: Of the 404 strains examined, 202 (50.00%), 199 (49.26%), and 3 (0.74%) strains were identified as M. abscessus, M. massiliense, and M. bolletii, respectively. Of the 152 clarithromycin-resistant strains, 6 possessed rrl mutations, while 4 of the 30 amikacin-resistant strains contained rrs mutations, and 5 of the 114 quinolone-resistant strains had gyr mutations. All mutant strains had high minimal inhibitory concentration values for the antibiotics. Conclusions: Our results showed the distribution of the strains with mutations in drug resistance-related genes was low in the M. abscessus group. Furthermore, we performed drug susceptibility testing and sequence analyses to determine the characteristics of these genes in the M. abscessus group.

KW - Drug resistance

KW - Mutation

KW - Mycobacterium abscessus group

UR - http://www.scopus.com/inward/record.url?scp=84893421504&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893421504&partnerID=8YFLogxK

U2 - 10.3343/alm.2014.34.1.31

DO - 10.3343/alm.2014.34.1.31

M3 - Article

VL - 34

SP - 31

EP - 37

JO - Annals of Laboratory Medicine

JF - Annals of Laboratory Medicine

SN - 2234-3806

IS - 1

ER -