The E3 ubiquitin ligase HOS1 regulates low ambient temperature-responsive flowering in arabidopsis thaliana

Jeong Hwan Lee, Jae Joon Kim, Soo Hyun Kim, Hyun Jung Cho, Joonki Kim, Ji Hoon Ahn

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Ubiquitin-dependent proteolysis regulates multiple aspects of plant growth and development, but little is known about its role in ambient temperature-responsive flowering. In addition to being regulated by daylength, the onset of flowering in many plants can also be delayed by low ambient temperatures. Here, we show that HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1 (HOS1), which encodes an E3 ubiquitin ligase, controls flowering time in response to ambient temperatures (16 and 23°C) and intermittent cold. hos1 mutants flowered early, and were insensitive to ambient temperature, but responded normally to vernalization and gibberellic acid. Genetic analyses suggested that this ambient temperature-insensitive flowering was independent of FLOWERING LOCUS C (FLC). Also, FLOWERING LOCUS T (FT) and TWIN SISTER OF FT (TSF) expression was up-regulated in hos1 mutants at both temperatures. The ft tsf mutation almost completely suppressed the early flowering of hos1 mutants at different temperatures, suggesting that FT and TSF are downstream of HOS1 in the ambient temperature response. A lesion in CONSTANS (CO) did not affect the ambient temperature-insensitive flowering phenotype of hos1-3 mutants. In silico analysis showed that FVE was spatiotemporally co-expressed with HOS1. A HOS1-green fluorescent protein (GFP) fusion co-localized with FVE-GFP in the nucleus at both 16 and 23°C. HOS1 physically interacted with FVE and FLK in yeast two-hybrid and co-immunoprecipitation assays. Moreover, hos1 mutants were insensitive to intermittent cold. Collectively, our results suggest that HOS1 acts as a common regulator in the signaling pathways that control flowering time in response to low ambient temperature.

Original languageEnglish
Pages (from-to)1802-1814
Number of pages13
JournalPlant and Cell Physiology
Volume53
Issue number10
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

ubiquitin-protein ligase
Ubiquitin-Protein Ligases
Arabidopsis
ambient temperature
Arabidopsis thaliana
flowering
Temperature
mutants
green fluorescent protein
Green Fluorescent Proteins
lesions (plant)
vernalization
ubiquitin
gibberellic acid
proteolysis
Plant Development
plant development
Ubiquitin
temperature
growth and development

Keywords

  • Ambient temperature
  • Cross-talk
  • E3 ubiquitin ligase
  • Flowering time
  • HOS1
  • Intermittent cold

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

The E3 ubiquitin ligase HOS1 regulates low ambient temperature-responsive flowering in arabidopsis thaliana. / Lee, Jeong Hwan; Kim, Jae Joon; Kim, Soo Hyun; Cho, Hyun Jung; Kim, Joonki; Ahn, Ji Hoon.

In: Plant and Cell Physiology, Vol. 53, No. 10, 01.10.2012, p. 1802-1814.

Research output: Contribution to journalArticle

Lee, Jeong Hwan ; Kim, Jae Joon ; Kim, Soo Hyun ; Cho, Hyun Jung ; Kim, Joonki ; Ahn, Ji Hoon. / The E3 ubiquitin ligase HOS1 regulates low ambient temperature-responsive flowering in arabidopsis thaliana. In: Plant and Cell Physiology. 2012 ; Vol. 53, No. 10. pp. 1802-1814.
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