The ectopic expression of methionine sulfoxide reductase 4 results in enhanced growth performance in arabidopsis

Jee Eun Oh, Md Aktar Hossain, Jun Hyeok Kim, Seung Hee Chu, Eun Hye Lee, Kwang Yeon Hwang, Hana Noh, Suk Whan Hong, Hojoung Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In response to challenging oxidative environments, most aerobic organisms have developed cellular defense systems that either repair or degrade damaged proteins to prevent them from adversely affecting cellular reactions. For instance, oxidized methionine can be reduced to methionine by the enzyme methionine sulfoxide reductase (MSR). Previously, we reported that the Arabidopsis gene MSR4 (AT4G25130) is induced by salt stress, and during our investigations into this gene, we generated a transgenic Arabidopsis that overexpressed a mutant form of MSR4 (mMSR4). The transgenic plants exhibited enhanced sensitivity to high salt stress. Consistent with this observation, the ectopic expression of MSR4 enhanced growth performance in normal as well as in stress medium. To determine the targets of MSR4, we performed two-dimensional electrophoresis analyses and observed a significant reduction in the levels of chloroplast heat shock proteins, which may explain the enhanced salt stress sensitivity of mMSR4 transgenic plants.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalPlant Science
Volume178
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

Fingerprint

Arabidopsis
salt stress
methionine
growth performance
Salts
Genetically Modified Plants
Methionine
transgenic plants
Growth
Chloroplast Proteins
mutants
Heat-Shock Proteins
heat shock proteins
Genes
electrophoresis
Electrophoresis
genes
chloroplasts
genetically modified organisms
organisms

Keywords

  • Arabidopsis thaliana
  • COR15a
  • Hsp70
  • MSR4
  • Salt stress

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Agronomy and Crop Science

Cite this

The ectopic expression of methionine sulfoxide reductase 4 results in enhanced growth performance in arabidopsis. / Oh, Jee Eun; Hossain, Md Aktar; Kim, Jun Hyeok; Chu, Seung Hee; Lee, Eun Hye; Hwang, Kwang Yeon; Noh, Hana; Hong, Suk Whan; Lee, Hojoung.

In: Plant Science, Vol. 178, No. 3, 01.03.2010, p. 265-270.

Research output: Contribution to journalArticle

Oh, Jee Eun ; Hossain, Md Aktar ; Kim, Jun Hyeok ; Chu, Seung Hee ; Lee, Eun Hye ; Hwang, Kwang Yeon ; Noh, Hana ; Hong, Suk Whan ; Lee, Hojoung. / The ectopic expression of methionine sulfoxide reductase 4 results in enhanced growth performance in arabidopsis. In: Plant Science. 2010 ; Vol. 178, No. 3. pp. 265-270.
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