The effect of alendronate-loaded polycarprolactone nanofibrous scaffolds on osteogenic differentiation of adipose-derived stem cells in bone tissue regeneration

Young Pil Yun, Seung Ju Kim, Youn Mook Lim, Kyeongsoon Park, Hak Jun Kim, Sung In Jeong, Sung Eun Kim, Hae Ryong Song

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The osteogenic effect of culturing adipose-derived stem cells (ADSCs) on alendronate (Aln)-loaded polycarprolactone (PCL) nanofibrous scaffolds was evaluated by examining alkaline phosphatase (ALP) activity, calcium content, and expression of osteogenic differentiation genes in vitro. The 10% Aln/PCL nanofibrous scaffolds showed more ALP activity, mineralization, and osteocalcin and osteopontin mRNA than the 1% or 5% Aln/PCL nanofibrous scaffolds. The capacity of Aln/PCL nanofibrous scaffolds to regenerate new bone was studied in a rat calvarial defect model. New bone formation in vivo was evaluated by radiography, micro-computed tomography, and histological analysis. At 8 weeks after implantation, Aln/PCL scaffolds had a positive effect on bone regeneration and matrix formation. These results suggested that Aln/PCL nanofibrous scaffolds enhanced the osteogenic differentiation of ADSCs in vitro and bone formation in vivo.

Original languageEnglish
Pages (from-to)1080-1090
Number of pages11
JournalJournal of Biomedical Nanotechnology
Volume10
Issue number6
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Alendronate
Tissue regeneration
Bone Regeneration
Stem cells
Scaffolds
Bone
Stem Cells
Bone and Bones
Phosphatases
Osteogenesis
Alkaline Phosphatase
Bone Matrix
Osteopontin
Radiography
Osteocalcin
Tomography
Rats
Calcium
Genes
Messenger RNA

Keywords

  • Adipose-Derived stem cells
  • Alendronate
  • Bone regeneration
  • Nanofiber
  • Polycarprolactone

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

The effect of alendronate-loaded polycarprolactone nanofibrous scaffolds on osteogenic differentiation of adipose-derived stem cells in bone tissue regeneration. / Yun, Young Pil; Kim, Seung Ju; Lim, Youn Mook; Park, Kyeongsoon; Kim, Hak Jun; Jeong, Sung In; Kim, Sung Eun; Song, Hae Ryong.

In: Journal of Biomedical Nanotechnology, Vol. 10, No. 6, 01.01.2014, p. 1080-1090.

Research output: Contribution to journalArticle

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