The effect of alendronate-loaded polycarprolactone nanofibrous scaffolds on osteogenic differentiation of adipose-derived stem cells in bone tissue regeneration

Young Pil Yun, Seung Ju Kim, Youn Mook Lim, Kyeongsoon Park, Hak Jun Kim, Sung In Jeong, Sung Eun Kim, Hae Ryong Song

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26 Citations (Scopus)

Abstract

The osteogenic effect of culturing adipose-derived stem cells (ADSCs) on alendronate (Aln)-loaded polycarprolactone (PCL) nanofibrous scaffolds was evaluated by examining alkaline phosphatase (ALP) activity, calcium content, and expression of osteogenic differentiation genes in vitro. The 10% Aln/PCL nanofibrous scaffolds showed more ALP activity, mineralization, and osteocalcin and osteopontin mRNA than the 1% or 5% Aln/PCL nanofibrous scaffolds. The capacity of Aln/PCL nanofibrous scaffolds to regenerate new bone was studied in a rat calvarial defect model. New bone formation in vivo was evaluated by radiography, micro-computed tomography, and histological analysis. At 8 weeks after implantation, Aln/PCL scaffolds had a positive effect on bone regeneration and matrix formation. These results suggested that Aln/PCL nanofibrous scaffolds enhanced the osteogenic differentiation of ADSCs in vitro and bone formation in vivo.

Original languageEnglish
Pages (from-to)1080-1090
Number of pages11
JournalJournal of Biomedical Nanotechnology
Volume10
Issue number6
DOIs
Publication statusPublished - 2014 Jan 1

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Keywords

  • Adipose-Derived stem cells
  • Alendronate
  • Bone regeneration
  • Nanofiber
  • Polycarprolactone

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

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