The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs)

Sung Eun Kim, Su Young Lee, Young Pil Yun, Jae Yong Lee, Kyeongsoon Park, Deok Won Lee, Hae Ryong Song

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The purpose of this study was to develop alendronate (Aln)-eluting Ti substrates to induce osteogenic differentiation of human buccal fat cells (HBFCs). The surface of pristine Ti was modified by dopamine (DOPA) and then heparin was grafted onto the aminated Ti surfaces to achieve the Aln-eluting Ti system. Aln was subsequently immobilized on the surface of heparinized Ti (Hep-Ti). Pristine Ti and surface-modified-Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. Osteogenic differentiation of HBFCs on the surface of pristine-Ti, Hep-Ti, Aln (1 mg)/Hep-Ti, and Aln (5 mg)/Hep-Ti was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Successful immobilization of Aln on Hep-Ti was confirmed by XPS and contact angle. Aln/Hep-Ti showed the sustained release for up to 28 days. Additionally, HBFCs cultured on Aln/Hep-Ti substrates showed significantly induced ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. These results suggest that Aln-eluting Ti substrates have a potential effect on osteogenic differentiation of HBFCs and will be a promising material for bone regeneration.

Original languageEnglish
Title of host publicationNanosystems in Engineering and Medicine
DOIs
Publication statusPublished - 2012
EventNanosystems in Engineering and Medicine - Incheon, Korea, Republic of
Duration: 2012 Sep 102012 Sep 12

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8548
ISSN (Print)1605-7422

Other

OtherNanosystems in Engineering and Medicine
CountryKorea, Republic of
CityIncheon
Period12/9/1012/9/12

Keywords

  • Alendronate
  • Human buccal fat cells (HBFCs)
  • Osteogenic differentiation
  • Pristine Ti
  • Surface modification

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Kim, S. E., Lee, S. Y., Yun, Y. P., Lee, J. Y., Park, K., Lee, D. W., & Song, H. R. (2012). The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs). In Nanosystems in Engineering and Medicine [85486B] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8548). https://doi.org/10.1117/12.979889