The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs)

Sung Eun Kim, Su Young Lee, Young Pil Yun, Jae Yong Lee, Kyeongsoon Park, Deok Won Lee, Hae Ryong Song

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The purpose of this study was to develop alendronate (Aln)-eluting Ti substrates to induce osteogenic differentiation of human buccal fat cells (HBFCs). The surface of pristine Ti was modified by dopamine (DOPA) and then heparin was grafted onto the aminated Ti surfaces to achieve the Aln-eluting Ti system. Aln was subsequently immobilized on the surface of heparinized Ti (Hep-Ti). Pristine Ti and surface-modified-Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. Osteogenic differentiation of HBFCs on the surface of pristine-Ti, Hep-Ti, Aln (1 mg)/Hep-Ti, and Aln (5 mg)/Hep-Ti was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Successful immobilization of Aln on Hep-Ti was confirmed by XPS and contact angle. Aln/Hep-Ti showed the sustained release for up to 28 days. Additionally, HBFCs cultured on Aln/Hep-Ti substrates showed significantly induced ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. These results suggest that Aln-eluting Ti substrates have a potential effect on osteogenic differentiation of HBFCs and will be a promising material for bone regeneration.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8548
DOIs
Publication statusPublished - 2012 Dec 1
EventNanosystems in Engineering and Medicine - Incheon, Korea, Republic of
Duration: 2012 Sep 102012 Sep 12

Other

OtherNanosystems in Engineering and Medicine
CountryKorea, Republic of
CityIncheon
Period12/9/1012/9/12

Fingerprint

Alendronate
Cheek
fats
Titanium
Oils and fats
Adipocytes
titanium
Fats
osteocalcin
cells
phosphatases
Phosphatases
Contact angle
calcium
Calcium
Substrates
X ray photoelectron spectroscopy
photoelectron spectroscopy
Photoelectron Spectroscopy
heparins

Keywords

  • Alendronate
  • Human buccal fat cells (HBFCs)
  • Osteogenic differentiation
  • Pristine Ti
  • Surface modification

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, S. E., Lee, S. Y., Yun, Y. P., Lee, J. Y., Park, K., Lee, D. W., & Song, H. R. (2012). The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs). In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8548). [85486B] https://doi.org/10.1117/12.979889

The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs). / Kim, Sung Eun; Lee, Su Young; Yun, Young Pil; Lee, Jae Yong; Park, Kyeongsoon; Lee, Deok Won; Song, Hae Ryong.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8548 2012. 85486B.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kim, SE, Lee, SY, Yun, YP, Lee, JY, Park, K, Lee, DW & Song, HR 2012, The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs). in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8548, 85486B, Nanosystems in Engineering and Medicine, Incheon, Korea, Republic of, 12/9/10. https://doi.org/10.1117/12.979889
Kim SE, Lee SY, Yun YP, Lee JY, Park K, Lee DW et al. The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs). In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8548. 2012. 85486B https://doi.org/10.1117/12.979889
Kim, Sung Eun ; Lee, Su Young ; Yun, Young Pil ; Lee, Jae Yong ; Park, Kyeongsoon ; Lee, Deok Won ; Song, Hae Ryong. / The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs). Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8548 2012.
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abstract = "The purpose of this study was to develop alendronate (Aln)-eluting Ti substrates to induce osteogenic differentiation of human buccal fat cells (HBFCs). The surface of pristine Ti was modified by dopamine (DOPA) and then heparin was grafted onto the aminated Ti surfaces to achieve the Aln-eluting Ti system. Aln was subsequently immobilized on the surface of heparinized Ti (Hep-Ti). Pristine Ti and surface-modified-Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. Osteogenic differentiation of HBFCs on the surface of pristine-Ti, Hep-Ti, Aln (1 mg)/Hep-Ti, and Aln (5 mg)/Hep-Ti was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Successful immobilization of Aln on Hep-Ti was confirmed by XPS and contact angle. Aln/Hep-Ti showed the sustained release for up to 28 days. Additionally, HBFCs cultured on Aln/Hep-Ti substrates showed significantly induced ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. These results suggest that Aln-eluting Ti substrates have a potential effect on osteogenic differentiation of HBFCs and will be a promising material for bone regeneration.",
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