The effect of doxycycline on PMA-induced MUC5B expression via MMP-9 and p38 in NCI-H292 cells

Chang Hoon Bae, Seung Min Chen, Heung Man Lee, Si Youn Song, Yong Dae Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives. Doxycycline is commonly used in medicine for its bacteriostatic antimicrobial properties. Recent studies have reported that doxycycline also has anti-inflammatory effects. Matrix metalloproteinase (MMP)-9 has been found to be involved in the physiological and pathological process of inflammatory airway disease. Phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, is known to stimulate the expression of MMP and mucin genes in the airway and intestinal epithelial cells. Therefore, the effects and signal pathways of doxycycline on PMA-induced MUC5B expression dependent MMP-9 in human airway epithelial cells were investigated. Methods. In human NCI-H292 airway epithelial cells, MUC5B and MMP-9 mRNA expression, MUC5B protein expression, and MMP-9 protein activity after the treatment with PMA, MMP-9 or doxycycline were determined by reverse transcriptase-polymerase chain reaction, enzyme immunoassay, gelatin zymography, and Western blot analysis. Results. PMA increased MMP-9 and MUC5B expression. MMP-9 increased MUC5B expression. Doxycycline inhibited PMA-induced MUC5B expression, and PMA-induced MMP-9 mRNA expression and protein activity. Doxycycline inhibited phosphorylation of p38 induced by PMA and MMP-9. Conclusion. The results of this study suggest that doxycycline inhibited PMA-induced MUC5B mRNA expression and protein production through the MMP-9 and p38 pathways in human NCI-H292 airway epithelial cells.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalClinical and Experimental Otorhinolaryngology
Volume4
Issue number4
DOIs
Publication statusPublished - 2011 Dec

Keywords

  • Doxycycline
  • Epithelial cells
  • Inflammation
  • MUC5B
  • Matrix metalloproteinase-9
  • Mucins
  • NCI-H292 cell
  • P38
  • Phorbol myristate acetate

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Fingerprint Dive into the research topics of 'The effect of doxycycline on PMA-induced MUC5B expression via MMP-9 and p38 in NCI-H292 cells'. Together they form a unique fingerprint.

  • Cite this