The effect of extracellular matrix on activation of atrocyte in 3D co-culture chip for nerve injury model

Young Hun Kim, Young Eun Kim, Seok Chung, Tae Song Kim, Ji Yoon Kang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper investigated the effect of extracellular matrix (ECM) on the morphology and activation status of astrocyte in microfluidic cell culture chip. The device provides a 3D co-culture system mimicking in vivo environment of central nervous system as well as axon isolation from neuron soma. Astrocyteswere seeded in collagen gel through small fluidic channels and neuron cells were aggregated at openings of small channel to large channels. This method not only separated neuronal soma from axon but also segregatedneuronalbody from astrocyte. The interaction of axons and astrocyte in the chip showed that the properties of extracellular matrix determines the activation status of astrocytes.The morphology of astrocytes was compared in two extracellular matrix such as collagen, a mixture of collagen andlaminin. The shape of astrocytes in laminin mixture was more star-like that was almost same as in-vivo astrocyte than that in collagen only.Also the effect of TGF-β1 was higher in laminin mixture, which imply that the astrocytes in laminin mixture were deactivated and those in collagen appeared quiescent. Theexperimental results showed thatthe activation status of astrocytes can be controlled in 3D cell culture chip by the control of extracellular matrix material and the treatment of TGF-β1. Hence the suggested co-culture chip is expected to provide a model of axon growth after the damageof nervous system like spinal cord injury.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1717-1719
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

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Keywords

  • 3D cell culture
  • Astrocyte
  • Co-culture
  • Extracellular matrix
  • Neuron

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kim, Y. H., Kim, Y. E., Chung, S., Kim, T. S., & Kang, J. Y. (2012). The effect of extracellular matrix on activation of atrocyte in 3D co-culture chip for nerve injury model. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1717-1719). Chemical and Biological Microsystems Society.