The effect of gelatin incorporation into electrospun poly(l-lactide-co-ε-caprolactone) fibers on mechanical properties and cytocompatibility

Jongman Lee, Giyoong Tae, Young Ha Kim, In Su Park, Sang Heon Kim, Soo Hyun Kim

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Very elastic poly(l-lactide-co-ε-caprolactone) (PLCL) (50:50) copolymer blended with gelatin was electrospun into microfibers from a hexafluoroisopropanol solution. PLCL fiber sheet exhibited the unique soft and flexible behavior while gelatin fiber was hard and brittle. As the gelatin content of PLCL/gelatin fibers increased, Young's modulus was increased, but the elongation was decreased compared to those of PLCL. However, fibers containing 10-30 wt% gelatin demonstrated an enhanced tensile strength with still high elongation to be beneficial for tissue engineering scaffolds. The cytocompatibility of electrospun fiber sheets was evaluated by fibroblasts (NIH-3T3) cell culture. The initial cell adhesion on various fibers after 5 h was somewhat similar, but in the order of PLCL > PLCL70/gelatin30 ≈ PLCL50/gelatin50 > PLCL90/gelatin10 ≈ gelatin > PLCL30/gelatin70. However, the cell proliferation exhibited a completely different and strong dependence on the fiber composition: a very high proliferation rate on PLCL90/gelatin10, followed by PLCL > gelatin > PLCL70/gelatin30. Such an enhanced effect of gelatin, especially at 10 wt% content, on strength and cytocompatibility of PLCL/gelatin fibers would be very preferable for tissue engineering scaffolds.

Original languageEnglish
Pages (from-to)1872-1879
Number of pages8
JournalBiomaterials
Volume29
Issue number12
DOIs
Publication statusPublished - 2008 Apr
Externally publishedYes

Keywords

  • Cytocompatibility
  • Electrospinning
  • Fibroblasts
  • PLCL-gelatin fibers

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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