The effect of gelatin incorporation into electrospun poly(l-lactide-co-ε-caprolactone) fibers on mechanical properties and cytocompatibility

Jongman Lee, Giyoong Tae, Young Ha Kim, In Su Park, Sang Heon Kim, Soo Hyun Kim

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Very elastic poly(l-lactide-co-ε-caprolactone) (PLCL) (50:50) copolymer blended with gelatin was electrospun into microfibers from a hexafluoroisopropanol solution. PLCL fiber sheet exhibited the unique soft and flexible behavior while gelatin fiber was hard and brittle. As the gelatin content of PLCL/gelatin fibers increased, Young's modulus was increased, but the elongation was decreased compared to those of PLCL. However, fibers containing 10-30 wt% gelatin demonstrated an enhanced tensile strength with still high elongation to be beneficial for tissue engineering scaffolds. The cytocompatibility of electrospun fiber sheets was evaluated by fibroblasts (NIH-3T3) cell culture. The initial cell adhesion on various fibers after 5 h was somewhat similar, but in the order of PLCL > PLCL70/gelatin30 ≈ PLCL50/gelatin50 > PLCL90/gelatin10 ≈ gelatin > PLCL30/gelatin70. However, the cell proliferation exhibited a completely different and strong dependence on the fiber composition: a very high proliferation rate on PLCL90/gelatin10, followed by PLCL > gelatin > PLCL70/gelatin30. Such an enhanced effect of gelatin, especially at 10 wt% content, on strength and cytocompatibility of PLCL/gelatin fibers would be very preferable for tissue engineering scaffolds.

Original languageEnglish
Pages (from-to)1872-1879
Number of pages8
JournalBiomaterials
Volume29
Issue number12
DOIs
Publication statusPublished - 2008 Apr 1
Externally publishedYes

Fingerprint

Gelatin
Mechanical properties
Fibers
Tissue Scaffolds
Scaffolds (biology)
Tissue engineering
Tissue Engineering
Elongation
dilactide
caprolactone
poly(lactide)
NIH 3T3 Cells
Cell adhesion
Cell proliferation
Fibroblasts
Tensile Strength
Elastic Modulus
Cell culture
Cell Adhesion
Tensile strength

Keywords

  • Cytocompatibility
  • Electrospinning
  • Fibroblasts
  • PLCL-gelatin fibers

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The effect of gelatin incorporation into electrospun poly(l-lactide-co-ε-caprolactone) fibers on mechanical properties and cytocompatibility. / Lee, Jongman; Tae, Giyoong; Kim, Young Ha; Park, In Su; Kim, Sang Heon; Kim, Soo Hyun.

In: Biomaterials, Vol. 29, No. 12, 01.04.2008, p. 1872-1879.

Research output: Contribution to journalArticle

Lee, Jongman ; Tae, Giyoong ; Kim, Young Ha ; Park, In Su ; Kim, Sang Heon ; Kim, Soo Hyun. / The effect of gelatin incorporation into electrospun poly(l-lactide-co-ε-caprolactone) fibers on mechanical properties and cytocompatibility. In: Biomaterials. 2008 ; Vol. 29, No. 12. pp. 1872-1879.
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