The effect of immobilization of heparin and bone morphogenic protein-2 to bovine bone substitute on osteoblast-like cell's function

Jung Bo Huh, Sung Eun Kim, Se Kyung Song, Mi Jung Yun, Ji Suk Shim, Jeong Yol Lee, Sang-Wan Shin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: This study was performed to investigate the ability of recombinant human-bone morphogenic protein-2 immobilized on a heparin-grafted bone substrate to enhance the osteoblastic functions. Materials and Methods: The Bio-Oss®, not coated with any material, was used as a control group. In rhBMP-2-Bio-Oss® group, rhBMP-2 was coated with Bio-Oss® using only deep and dry methods (50 ng/mL, 24 h). In heparinized rhBMP-2-Bio-Oss® group, dopamine was anchored to the surface of Bio-Oss®, and coated with heparin. rhBMP-2 was immobilized onto the heparinized- Bio-Oss® surface. The release kinetics of the rhBMP-2-Bio-Oss® and heparinized rhBMP-2-Bio-Oss® were analyzed using an enzyme-linked immunosorbent assay. The biological activities of the MG63 cells on the three groups were investigated via cytotoxicity assay, cell proliferation assay, alkaline phosphatase (ALP) measurement, and calcium deposition determination. Statistical comparisons were carried out by one-way ANOVA test. Differences were considered statistically significant at *P<.05 and **P<.001. Results. The heparinized rhBMP-2-Bio-Oss® showed more sustained release compared to the rhBMP-2-Bio-Oss® over an extended time. In the measurement of the ALP activity, the heparinized group showed a significantly higher ALP activity when compared with the non-heparinized groups (P<.05). The MG63 cells cultivated in the group with rhBMP-2 showed increased calcium deposition, and the MG63 cells from the heparinized group increased more than those that were cultivated in the non-heparinized groups. Conclusion. Heparin increased the rhBMP-2 release amount and made sustained release possible, and heparinized Bio-Oss® with rhBMP-2 successfully improved the osteoblastic functions.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalJournal of Advanced Prosthodontics
Volume3
Issue number3
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Bone Substitutes
Osteoblasts
Immobilization
Heparin
Bone and Bones
Proteins
Alkaline Phosphatase
Bio-Oss
Immobilized Proteins
Calcium
Dopamine
Analysis of Variance
Enzyme-Linked Immunosorbent Assay
Cell Proliferation

Keywords

  • Bovine bone
  • Heparin
  • Osteoblast-like cell
  • RhBMP-2

ASJC Scopus subject areas

  • Dentistry (miscellaneous)
  • Oral Surgery

Cite this

The effect of immobilization of heparin and bone morphogenic protein-2 to bovine bone substitute on osteoblast-like cell's function. / Huh, Jung Bo; Kim, Sung Eun; Song, Se Kyung; Yun, Mi Jung; Shim, Ji Suk; Lee, Jeong Yol; Shin, Sang-Wan.

In: Journal of Advanced Prosthodontics, Vol. 3, No. 3, 01.12.2011, p. 145-151.

Research output: Contribution to journalArticle

Huh, Jung Bo ; Kim, Sung Eun ; Song, Se Kyung ; Yun, Mi Jung ; Shim, Ji Suk ; Lee, Jeong Yol ; Shin, Sang-Wan. / The effect of immobilization of heparin and bone morphogenic protein-2 to bovine bone substitute on osteoblast-like cell's function. In: Journal of Advanced Prosthodontics. 2011 ; Vol. 3, No. 3. pp. 145-151.
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AU - Kim, Sung Eun

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AU - Yun, Mi Jung

AU - Shim, Ji Suk

AU - Lee, Jeong Yol

AU - Shin, Sang-Wan

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N2 - Purpose: This study was performed to investigate the ability of recombinant human-bone morphogenic protein-2 immobilized on a heparin-grafted bone substrate to enhance the osteoblastic functions. Materials and Methods: The Bio-Oss®, not coated with any material, was used as a control group. In rhBMP-2-Bio-Oss® group, rhBMP-2 was coated with Bio-Oss® using only deep and dry methods (50 ng/mL, 24 h). In heparinized rhBMP-2-Bio-Oss® group, dopamine was anchored to the surface of Bio-Oss®, and coated with heparin. rhBMP-2 was immobilized onto the heparinized- Bio-Oss® surface. The release kinetics of the rhBMP-2-Bio-Oss® and heparinized rhBMP-2-Bio-Oss® were analyzed using an enzyme-linked immunosorbent assay. The biological activities of the MG63 cells on the three groups were investigated via cytotoxicity assay, cell proliferation assay, alkaline phosphatase (ALP) measurement, and calcium deposition determination. Statistical comparisons were carried out by one-way ANOVA test. Differences were considered statistically significant at *P<.05 and **P<.001. Results. The heparinized rhBMP-2-Bio-Oss® showed more sustained release compared to the rhBMP-2-Bio-Oss® over an extended time. In the measurement of the ALP activity, the heparinized group showed a significantly higher ALP activity when compared with the non-heparinized groups (P<.05). The MG63 cells cultivated in the group with rhBMP-2 showed increased calcium deposition, and the MG63 cells from the heparinized group increased more than those that were cultivated in the non-heparinized groups. Conclusion. Heparin increased the rhBMP-2 release amount and made sustained release possible, and heparinized Bio-Oss® with rhBMP-2 successfully improved the osteoblastic functions.

AB - Purpose: This study was performed to investigate the ability of recombinant human-bone morphogenic protein-2 immobilized on a heparin-grafted bone substrate to enhance the osteoblastic functions. Materials and Methods: The Bio-Oss®, not coated with any material, was used as a control group. In rhBMP-2-Bio-Oss® group, rhBMP-2 was coated with Bio-Oss® using only deep and dry methods (50 ng/mL, 24 h). In heparinized rhBMP-2-Bio-Oss® group, dopamine was anchored to the surface of Bio-Oss®, and coated with heparin. rhBMP-2 was immobilized onto the heparinized- Bio-Oss® surface. The release kinetics of the rhBMP-2-Bio-Oss® and heparinized rhBMP-2-Bio-Oss® were analyzed using an enzyme-linked immunosorbent assay. The biological activities of the MG63 cells on the three groups were investigated via cytotoxicity assay, cell proliferation assay, alkaline phosphatase (ALP) measurement, and calcium deposition determination. Statistical comparisons were carried out by one-way ANOVA test. Differences were considered statistically significant at *P<.05 and **P<.001. Results. The heparinized rhBMP-2-Bio-Oss® showed more sustained release compared to the rhBMP-2-Bio-Oss® over an extended time. In the measurement of the ALP activity, the heparinized group showed a significantly higher ALP activity when compared with the non-heparinized groups (P<.05). The MG63 cells cultivated in the group with rhBMP-2 showed increased calcium deposition, and the MG63 cells from the heparinized group increased more than those that were cultivated in the non-heparinized groups. Conclusion. Heparin increased the rhBMP-2 release amount and made sustained release possible, and heparinized Bio-Oss® with rhBMP-2 successfully improved the osteoblastic functions.

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KW - Heparin

KW - Osteoblast-like cell

KW - RhBMP-2

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